Abstract
[Objective] To shorten the extraction cycle of Lentinus edodes polysaccharides and increase their content. [Methods] L.edodes mycelia are used as the raw material for liquid -state fermentation. With mycelial biomass and mycelial polysaccharide content as evaluation indicators, the liquid -state fermentation process is optimized by response surface methodology. The in vitro physiological activities of the obtained polysac charides are then evaluated. [Results] The optimal process conditions for liquid -state fermentation of L.edodes mycelial polysaccharides are corn starch of 43 g/L, yeast extract of 20 g/L, FeSO4 of 2 g/L, and fermentation with shaking at 150 r/min for 7 days. Under these conditions, the mycelial polysaccharide content and mycelial biomass of L.edodes are 3.81% and 7.33 g/L, respectively. In addition, the polysaccharides at 2.5 g/L show the DPPH radical scavenging rate of 90.46% and the inhibition zone diameters of 11.57 mm and 10.71 mm against Escherichia coli and Saccharomyces cerevisiae, respectively. [Conclusion] This liquid -state fermentation process enables the efficient production of L.edodes mycelial polysaccharides, which have strong DPPH radical scavenging ability and antibacterial effects.
Publication Date
11-3-2026
First Page
178
Last Page
186
DOI
10.13652/j.spjx.1003.5788.2025.80993
Recommended Citation
Duan, Yang and Zhi, Li
(2026)
"Liquid-state fermentation process optimization and in vitro physiological activity evaluation of Lentinus edodes polysaccharides,"
Food and Machinery: Vol. 42:
Iss.
10, Article 21.
DOI: 10.13652/j.spjx.1003.5788.2025.80993
Available at:
https://www.ifoodmm.cn/journal/vol42/iss10/21
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