Abstract
[Objective] To selectively screen lactic acid bacteria with high diacetyl yield and explore the effect of low -frequency ultrasound treatment on their diacetyl synthesis capacity. [Methods] Using diacetyl yield as the indicator, high -yielding strains were selectively isolated from strains preserved in the laboratory. The strains were identified by 16S rDNA gene sequencing and Gram staining. The target strain was evaluated for safety, including antibiotic susceptibility, hemolytic activity, and biogenic amine production, and its probiotic potential was characterized based on free radical scavenging capacity and tolerance to simulated gastrointestinal conditions. The diacetyl synthesis capacity of the strain was improved by optimizing the ultrasonic pretreatment conditions. [Results] Enterococcus faecalis L402 was obtained, with an initial diacetyl yield of 13.89 μg/mL. The strain showed no resistance to penicillin or vancomycin and exhibited no hemolytic activity;No biogenic amines, such as histamine or cadaverine, were detected. The scavenging rates of ABTS+ free radicals, superoxide anion free radicals, and DPPH free radicals by the intact cell suspension were 93.49%, 90.74%, and 78.97%, respectively. The survival rates in simulated gastric juice for 2 h and simulated intestinal juice for 4 h were both greater than 60%. After optimization of the low-frequency ultrasound treatment conditions, the optimal process parameters were an ultrasonic power of 150 W, an ultrasonic treatment time of 20 min, and an ultrasonic treatment temperature of 42 ℃. [Conclusion] E.faecalis L402 possesses both safety and probiotic potential, and low -frequency ultrasound treatment combined with commercial starter cultures can effectively enhance its diacetyl synthesis capacity. |
Publication Date
11-3-2026
First Page
28
Last Page
37
DOI
10.13652/j.spjx.1003.5788.2025.81016
Recommended Citation
Xinyi, Li; Hui, Zhou; Shunhong, Hu; Binrong, Sun; Xia, Tang; Jie, Luo; and Xiankang, Fan
(2026)
"Screening and identification of high-yield diacetyl lactic acid bacteria and optimization of the ultrasonic pretreatment process,"
Food and Machinery: Vol. 42:
Iss.
10, Article 4.
DOI: 10.13652/j.spjx.1003.5788.2025.81016
Available at:
https://www.ifoodmm.cn/journal/vol42/iss10/4
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