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Authors

MI Hongbo, College of Food Science and Technology, Bohai University, Jinzhou, Liaoning 121013, China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning 121013, China
FENG Mengmeng, College of Food Science and Technology, Bohai University, Jinzhou, Liaoning 121013, China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning 121013, China
CHEN Jingxin, College of Food Science and Technology, Bohai University, Jinzhou, Liaoning 121013, China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning 121013, China
LIU Chao, College of Food Science and Technology, Bohai University, Jinzhou, Liaoning 121013, China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning 121013, China
LI Jianrong, College of Food Science and Technology, Bohai University, Jinzhou, Liaoning 121013, China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning 121013, China
LI Xuepeng, College of Food Science and Technology, Bohai University, Jinzhou, Liaoning 121013, China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning 121013, ChinaFollow

Corresponding Author(s)

李学鹏(1982—), 男, 渤海大学教授, 博士。E-mail: xuepengli8234@163.com

Abstract

[Objective] To investigate the optimal concentration of myrcene and prolong the time of keeping sea cucumbers alive with low-temperature and water. [Methods] The effect of of myrcene concentrations (0, 10, 20, 30, 40, 50 µL/L) on the pH, hardness and soluble protein content of sea cucumbers during keeping alive with water is analyzed. The changes in sensory scores, body wall moisture status and distribution, and microstructure of sea cucumbers before and after keeping alive are compared. The effect of myrcene with the optimal concentration on the immune and antioxidant enzyme activities of sea cucumbers during keeping alive [Results] As the keeping alive time prolongs, the hardness and soluble protein content of the sea cucumber body wall gradually decrease. With the same preservation duration, the pH, hardness, and soluble protein content of the sea cucumber body wall in the 30 µL/L myrcene treatment group are significantly higher than those in the control group. With the longer keeping alive time, the activities of alkaline phosphatase, superoxide dismutase, catalase, and total antioxidant capacity in the sea cucumber intestine initially increase and then decrease, reaching their peaks at 6 or 8 days of preservation, while the enzyme activity changes in the 30 µL/L myrcene treatment group are significantly lower than those in the control group. Compared with the control group, the sea cucumbers after keeping alive for 12 d in 30 µL/L myrcene water solution are significantly higher in sensory scores and body wall-bound water contents. The myofibrillar tissues remain intact and autolysis is inhibited. [Conclusion] 30 µL/L myrcene solution could effectively alleviate the stress response of sea cucumbers during keeping alive with water and improve the quality of sea cucumbers.

Publication Date

4-3-2026

First Page

109

Last Page

117

DOI

10.13652/j.spjx.1003.5788.2025.80315

References

[1] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产 学 会.中 国 渔 业 统 计 年 鉴 [M].北 京:中 国 农 业 出 版 社,2025:23.Fisheries Administration of Ministry of Agriculture and Rural Affairs of the People's Republic of China,National Fisheries Technology Extension Center,China Society of Fisheries.China fishery statistical yearbook [M].Beijing:China Agriculture Press,2025:23.
[2] GIANASI B L,HAMEL J F,MERCIER A.Experimental test of optimal holding conditions for live transport of temperate sea cucumbers [J].Fisheries Research,2016,174:298-308.
[3] 林雅文,李艾靑,谭卓弘,等.干燥条件对干海参复水动力学及品质的影响 [J].中国食品学报,2025,25(2):280-289.LIN Y W,LI A Q,TAN Z H,et al.Effects of drying conditions on rehydration kinetics and quality of dried sea cucumber [J].Journal of Chinese Institute of Food Science and Technology,2025,25(2):280-289.
[4] ZHOU Y,ZHENG J,ZHAO J,et al.Oxygenated storage alleviates autolysis of the sea cucumber Apostichopus japonicus during transport [J].Aquaculture International,2023,31(5):2 779-2 798.
[5] XING J,ZHANG M D,ZHENG J,et al.Effect of extra oxygen on Apostichopus japonicus life sustainability during commercial transport [J].Food Control,2021,125:108022.
[6] 胡炜,赵斌,李成林,等.慢性氨氮胁迫对刺参摄食与消化酶活性的影响 [J].中国水产科学,2018,25(1):137-146.HU W,ZHAO B,LI C L,et al.Effects of chronic ammonia nitrogen stress on the feeding and digestive enzyme activities of sea cucumber (Apostichopus japonicas Selenka) [J].Journal of Fishery Sciences of China,2018,25(1):137-146.
[7] EBRAHIMZADEH MOUSAVI H,TAHERI MIRGHAED A,HOSEINI S M,et al.Myrcene as water conditioner,stress-reducing and antioxidant agent in transportation of common carp,Cyprinus carpio,with plastic bags [J].Aquaculture Reports,2023,28:101458.
[8] HOSEINI S M,YOUSEFI M,HOSEINIFAR S H,et al.Antioxidant,enzymatic and hematological responses of common carp (Cyprinus carpio) fed with myrcene-or menthol-supplemented diets and exposed to ambient ammonia [J].Aquaculture,2019,506:246-255.
[9] HOSEINI S M,KHALILI M,RAJABIESTERABADI H,et al.Effects of dietary monoterpene,myrcene,administration on immune-and health-related genes expression in common carp gill following exposure to copper sulfate [J].Fish & Shellfish Immunology,2020,98:438-445.
[10] KHALILI M,ATTAR M,AMIRLATIFI R,et al.Effects of dietary myrcene administration on antioxidant gene responses in common carp (Cyprinus carpio),exposed to copper sulphate[J].Aquaculture Research,2020,51(4):1 653-1 659.
[11] 朱璐璐,农绍庄,李江阔,等.两种生物保鲜液对鲜活海参贮藏品质的影响 [J].食品工业,2015,36(6):128-132.ZHU L L,NONG S Z,LI J K,et al.Effect of two kinds of biological preservation solution on the storage quality of fresh sea cucumber [J].The Food Industry,2015,36(6):128-132.
[12] 高汉朴,苏兆新,刘婷,等.转谷氨酰胺酶添加量及暂存条件对传统鱼糕品质的影响 [J].食品与机械,2025,41(6):121-128.GAO H P,SU Z X,LIU T,et al.Effects of transglutaminase addition amount and temporary storage conditions on the quality of traditional fish cake [J].Food & Machinery,2025,41(6):121-128.
[13] JØRPELAND G,IMSLAND A,STIEN L H,et al.Effects of filleting method,stress,storage and season on the quality of farmed Atlantic cod (Gadus morhua L) [J].Aquaculture Research,2015,46(7):1 597-1 607.
[14] 米红波,张婷,鹿悦,等.不同氧气含量条件下无水保活珍珠龙 胆 石 斑 鱼 品 质 预 测 模 型 的 建 立 [J].食 品 科 学 技 术 学 报,2024,42(2):162-176.MI H B,ZHANG T,LU Y,et al.Establishment of quality prediction model of pearl gentian grouper during waterless keeping alive with different oxygen content [J].Journal of Food Science and Technology,2024,42(2):162-176.
[15] 张美迪.海参运输过程中最佳保活条件的研究 [D].大连:大连工业大学,2020:39.ZHANG M D.The optimal transportation condition study of alive sea cucumber [D].Dalian:Dalian Polytechnic University,2020:39.
[16] 肖水水,张鹏,李江阔,等.不同浓度食用级木醋液对鲜活海参保鲜效果的研究 [J].食品科技,2016,41(5):35-40.XIAO S S,ZHANG P,LI J K,et al.Effect on fresh-keeping of fresh sea cucumber with different concentration edible wood vinegar [J].Food Science and Technology,2016,41(5):35-40.
[17] 王艳杰.抗坏血酸在海参保鲜保活运输中的作用及机制研究[D].大连:大连工业大学,2022:18.WANG Y J.Study on the effect and mechanism of ascorbic acid on maintaining fresh and alive during sea cucumber transportation [D].Dalian:Dalian Polytechnic University,2022:18.
[18] CHENG S W,ZHAO J,WANG Y J,et al.The impact of oxygen and ascorbic acid treatments on the physicochemical attributes of live sea cucumber during extended transportation/storage [J].Journal of Aquatic Food Product Technology,2024,33(4):315-327.
[19] CAO J,WANG Q,MEI J,et al.Effect of 3-aminobenzoic acid ethyl ester methanesulfonate (MS- 222) on quality of marine cultured turbot (Scophthalmus maximus) during simulated transport in water [J].Fishes,2021,6(2):20.
[20] COTTIN D,SHILLITO B,CHERTEMPS T,et al.Identification of differentially expressed genes in the hydrothermal vent shrimp Rimicaris exoculata exposed to heat stress [J].Marine Genomics,2010,3(2):71-78.
[21] 李根瑞.仿刺参对环境低氧胁迫的生理生化响应研究 [D].上海:上海海洋大学,2016:4.LI G R.Study on the physiological and biochemical responses of Apostichopus japouicus to environmental hypoxia stress [D].|Shanghai:Shanghai Ocean University,2016:4.
[22] CAI H,ZHAO J,WANG L,et al.Ascorbic acid alleviates the autolysis of the sea cucumber Apostichopus japonicus via the activation of antioxidant enzymes to remove reactive oxygen species during live storage [J].Aquaculture International,2024,32(4):4 207-4 224.
[23] 米红波,鹿悦,陈敬鑫,等.牛磺酸和谷氨酰胺对石斑鱼的应激缓解作用 [J].水产科学,2024,43(6):865-874.MI H B,LU Y,CHEN J X,et al.Stress relieving effect of taurine and glutamine on grouper hybrid [J].Fisheries Science,2024,43(6):865-874.
[24] SHI G P,GAO T Q,LI X H,et al.Integrating transcriptomic and metabolomic analysis to understand muscle qualities of red swamp crayfish (Procambarus clarkii) under transport stress [J].Food Research International,2023,164:112361.
[25] 胡筱波,陈文飞,顾泽茂,等.抗冻剂对蒸煮小龙虾虾仁冻融后品质的影响 [J].食品与机械,2023,39(1):116-124,151.HU X B,CHEN W F,GU Z M,et al.Effect of antifreeze on the quality of cooked crawfish tail meat after freeze-thawed [J].Food & Machinery,2023,39(1):116-124,151.
[26] LIU Z L,XIE L,ZIELINSKA M,et al.Pulsed vacuum drying enhances drying of blueberry by altering micro-,ultrastructure and water status and distribution [J].LWT-Food Science and Technology,2021,142:111013.
[27] LI S,ZHOU Y,SUN L M,et al.Effects of storage method on the quality of processed sea cucumbers (Apostichopus japonicus) [J].Foods,2022,11(24):4 098.
[28] CHENG S S,WANG X H,LI R R,et al.Influence of multiple freeze-thaw cycles on quality characteristics of beef semimembranous muscle:with emphasis on water status and distribution by LF-NMR and MRI [J].Meat Science,2019,147:44-52.
[29] CHENG S S,WANG X H,YANG H M,et al.Characterization of moisture migration of beef during refrigeration storage by low-field NMR and its relationship to beef quality [J].Journal of the Science of Food and Agriculture,2020,100(5):1 940-1 948.
[30] 何秀.基于微波真空干燥过程的海参水分迁移及品质变化规律研究 [D].大连:大连工业大学,2021:22-25.HE X.Moisture migration and quality change of sea cucumber based on microwave vacuum drying process [D].Dalian:Dalian Polytechnic University,2021:22-25.
[31] 邢洁.海参加氧保鲜保活运输过程中组织结构和相关代谢酶变化的研究 [D].大连:大连工业大学,2021:50.XIE J.Study on the changes of tissue structure and related metabolic enzymes of sea cucumber during the process of oxygenated preservation and alive transportation [D].Dalian:Dalian Polytechnic University,2021:50.
[32] CHAN Q X,WANG F Q,HAN Y Z,et al.An investigation on dietary chromium picolinate supplementation in the juvenile sea cucumber Apostichopus japonicus:growth,digestive enzyme activity,growth-related genes expression,immune and antioxidant capacity [J].Aquaculture Reports,2022,24:101099.
[33] GUO J S,WU P H,CAO J L,et al.The PFOS disturbed immunomodulatory functions via nuclear Factor- κB signaling in liver of zebrafish (Danio rerio) [J].Fish & Shellfish Immunology,2019,91:87-98.
[34] HUO D,SUN L N,RU X S,et al.Impact of hypoxia stress on the physiological responses of sea cucumber Apostichopus japonicus:respiration,digestion,immunity and oxidative damage [J].PeerJ,2018,6:4 651.
[35] 王旖雪,覃思,周润泽,等.铁皮石斛典型黄酮抗氧化活性及分子机制研究 [J].食品与机械,2025,41(7):130-136.WANG Y X,QIN S,ZHOU R Z,et al.Antioxidant activity and molecular mechanisms of typical flavonoids in Dendrobium officinale [J].Food & Machinery,2025,41(7):130-136.
[36] 陈旭,左涛,周胜杰,等.饲喂甘草后低盐胁迫对尖吻鲈相关酶活性的影响 [J].水产科学,2023,42(3):404-412.CHEN X,ZUO T,ZHOU S J,et al.Effects of dietary radix glycyrrhizae powder on antioxidant capacity of Asian seabass Lates calcarifer under low salinity stress [J].Fisheries Science,2023,42(3):404-412.

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