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Corresponding Author(s)

艾民珉(1993—), 男, 华南农业大学首聘副教授, 博士。E-mail: scauaiminmin@scau.edu.cn

Abstract

Egg white, valued for its high protein content and superior food functional properties, is widely utilized in food processing. The amphiphilic structure and surface activity of egg white proteins enable effective adsorption at oil-water interfaces, reducing interfacial tension and forming stable interfacial films. Therefore, egg white proteins exhibit a robust interfacial regulation capacity in emulsion systems. This review systematically outlines the characteristics and formation mechanisms of multi-scale emulsion systems of egg white proteins (nano-emulsions, Pickering emulsions, and high internal phase Pickering emulsions). Additionally, a summary is presented of the research progress on the chemical/physical modification mechanisms of egg white emulsification performance, as well as their role in modifying egg white emulsification properties.

Publication Date

4-3-2026

First Page

206

Last Page

215

DOI

10.13652/j.spjx.1003.5788.2025.80392

References

[1] 徐 桂 云.鸡 蛋 品 质 及 营 养 价 值 的 新 认 识 [J].中 国 家 禽,2012,34(13):36-38.XU G Y.New understanding of egg quality and nutritional value[J].China Poultry,2012,34(13):36-38.
[2] 王晓翠,武书庚,张海军,等.鸡蛋蛋清品质营养调控的研究进展 [J].动物营养学报,2019,31(4):1 491-1 498.WANG X C,WU S G,ZHANG H J,et al.Nutritional modulation of egg albumen quality of laying hens:a review [J].Chinese Journal of Animal Nutrition,2019,31(4):1 491-1 498.
[3] 望运滔,王营娟,白艳红.预处理技术改善蛋白质乳化性研究进展 [J].食品与机械,2020,36(5):211-215.WANG Y T,WANG Y J,BAI Y H.Recent advances in pretreatment techniques to improve the emulsifying property of proteins [J].Food & Machinery,2020,36(5):211-215.
[4] 周玲,谢爱英,汪学荣.酶解对蛋清蛋白液起泡性能和乳化性能的影响 [J].食品工业科技,2012,33(24):103-105.ZHOU L,XIE A Y,WANG X R.Effects of hydrolysis on the foaming and emulsifying properties of egg white protein [J].Science and Technology of Food Industry,2012,33(24):103-105.
[5] 杨建荣,吴越,焦涵,等.物理、化学、生物法处理蛋黄颗粒对其乳化特性影响的研究进展 [J].现代食品科技,2022,38(12):129-138.YANG J R,WU Y,JIAO H,et al.Research progress in the emulsifying properties of egg yolk granules after physical,chemical,and biological treatments [J].Modern Food Science and Technology,2022,38(12):129-138.
[6] 孙乐常,曾添,林端权,等.干燥方式对蛋清蛋白理化性质和功能特性的影响 [J].食品工业科技,2022,43(24):102-111.SUN L C,ZENG T,LIN D Q,et al.Effects of different drying methods on physicochemical and functional properties of egg white protein [J].Science and Technology of Food Industry,2022,43(24):102-111.
[7] 李晶晶,蔡为荣,朱樱,等.酶解絮凝耦联对咸蛋清脱盐及理化性质的影响 [J].中国食品学报,2023,23(9):202-212.LI J J,CAI W R,ZHU Y,et al.Effect of enzymatic flocculation coupling on the desalination and physicochemical properties of salted egg whites [J].Journal of Chinese Institute of Food Science and Technology,2023,23(9):202-212.
[8] 张川,李倩文,王晓楠,等.蛋清蛋白 /黄油复合凝胶的特性研究[J].食品与发酵工业,2024,50(7):151-156.ZHANG C,LI Q W,WANG X N,et al.Study on the properties of egg white protein-butter compound gel [J].Food and Fermentation Industries,2024,50(7):151-156.
[9] 赵 笑 蕾,杨 锋,张 晓.水 力 空 化 对 蛋 清 蛋 白 功 能 特 性 的 影 响[J].食品科技,2019,44(10):105-109.ZHAO X L,YANG F,ZHANG X.Effects of hydrodynamic cavitation on functional characteristics of egg white protein [J].Food Science and Technology,2019,44(10):105-109.
[10] 关玉.pH-shifting 诱导蛋清蛋白结构和乳化特性变化及其在乳液中的应用研究 [D].长春:吉林大学,2023:1-2.GUAN Y.Structure and emulsifying properties of egg white protein induced by pH-shifting and its application in emulsion[D].Changchun:Jilin University,2023:1-2.
[11] 康钰钊,陶烁同,邓思惠,等.壳聚糖 /阿拉伯胶纳米乳液的制备、表征及稳定性研究 [J].食品与机械,2025,41(1):2-8.KANG Y L,TAO S T,DENG S H,et al.Preparation,characterization and stability of chitosan/gum Arabic nano-emulsion [J].Food & Machinery,2025,41(1):2-8.
[12] QADIR A,FAIYAZUDDIN M D,TALIB HUSSAIN M D,et al.Critical steps and energetics involved in a successful development of a stable nanoemulsion [J].Journal of Molecular Liquids,2016,214:7-18.
[13] RAO S Q,XU G W,ZENG H W,et al.Physicochemical and antibacterial properties of fabricated ovalbumin-carvacrol gel nanoparticles [J].Food & Function,2020,11(6):5 133-5 141.
[14] LONG Y Z,ZHANG M,YANG C H.Effects of flaxseed gum/egg white protein-based edible curcumin nanoemulsion coatings on starch retrogradation and quality of fried rice in freeze-thaw treatment [J].Food Bioscience,2024,59:103977.
[15] XIA T H,XUE C H,WEI Z H.Physicochemical characteristics,applications and research trends of edible Pickering emulsions [J].Trends in Food Science & Technology,2021,107:1-15.
[16] ZHAN Y L,QIN S Y,ZENG Y,et al.Pickering emulsions stabilized by egg white protein-alginate nanoparticles:freeze-thaw stability after heating [J].Food Hydrocolloids,2024,150:109675.
[17] 潘晴楣,张志鹏,冉乐童,等.蛋清蛋白 Pickering 乳液制备及其负载β-胡萝卜素的稳定性 [J].中国食品学报,2023,23(3):271-277.PAN Q M,ZHANG Z P,RAN L T,et al.Preparation of Pickering emulsion stabilized by egg white protein particles and the stability of β-carotene loaded [J].Journal of Chinese Institute of Food Science and Technology,2023,23(3):271-277.
[18] 王春颖.蛋清蛋白高内相皮克林乳液的制备及其包埋姜黄素的性能研究 [D].长春:吉林大学,2020:28-31.WANG C Y.Preparation of high internal phase Pickering emulsion stabilized by egg white protein and its properties of embedding curcumin [D].Changchun:Jilin University,2020:28-31.
[19] 郝梦,毛书灿,汪兰,等.食品中动物蛋白形成皮克林乳液的研究进展 [J].中国食品学报,2023,23(6):420-430.HAO M,MAO S C,WANG L,et al.Research progress on formation of Pickering emulsion from animal protein in food[J].Journal of Chinese Institute of Food Science and Technology,2023,23(6):420-430.
[20] 朱雨晴,刘伟,陈兴,等.食品级皮克林乳液的稳定机制及稳定性研究进展 [J].食品工业科技,2018,39(7):315-322.ZHU Y Q,LIU W,CHEN X,et al.Advances in stabilization mechanism and stability control of food grade Pickering emulsion [J].Science and Technology of Food Industry,2018,39(7):315-322.
[21] 田怀香,卢卓彦,胡静.食品级皮克林乳液制备及应用研究进展 [J].中国食品学报,2018,18(1):225-232.TIAN H X,LU Z Y,HU J.Preparation and application of food-grade pickering emulsion [J].Journal of Chinese Institute of Food Science and Technology,2018,18(1):225-232.
[22] 郭健.加工方式对酪蛋白 /蛋清蛋白基高内相乳液的稳定与应用特征研究 [D].长春:吉林大学,2023:4,29-30.GUO J.Study on the stability and application characteristics of casein/egg white protein-based high internal phase Pickering emulsions with various processing methods [D].Changchun:Jilin University,2023:4,29-30.
[23] ZHANG J N,ZHAO S Q,LI L T,et al.High internal phase emulsions stabilized by pea protein isolate modified by ultrasound combined with pH-shifting:micromorphology,rheology,and physical stability [J].Foods,2023,12(7):1 433.|[24] YANG J,KIM H,SUNG M,et al.High internal phase emulsion stabilization through restricted interdrop fusion across water drainage channels [J].Langmuir,2023,39(16):5 670-5 678.
[25] 徐丽兰.蛋黄—变性淀粉复合高内相乳液的构建、形成机制及应用研究 [D].无锡:江南大学,2022:3-4.XU L L.Study on construction,formation mechanism and applications of high internal phase emulsions stabilized by egg yolk-modified starch [D].Wuxi:Jiangnan University,2022:3-4.
[26] 米思杰.蛋黄颗粒高内相乳液体系构建及其对食品基质的稳态调控的研究 [D].武汉:华中农业大学,2022:9-11.MI S J.Construction of egg yolk granules-stabilized high internal phase emulsion system and research on homeostatic regulation of food matrices [D].Wuhan:Huazhong Agricultural University,2022:9-11.
[27] YANG Y Y,LI J H,SU Y J,et al.Composite emulsifying behavior of egg white protein and rhamnolipid:properties of the constructed high internal phase emulsions [J].Food Hydrocolloids,2022,123:106913.
[28] ZHANG T,LI S L,YANG M,et al.The influence of unique interfacial networks based on egg white proteins for the stabilization of high internal phase Pickering emulsions:physical stability and free fatty acid release kinetics [J].Food Chemistry,2024,442:138448.
[29] 张根生,苏文文,徐桂杨,等.低聚糖改性对蛋清蛋白凝胶性影响研究进展 [J].食品与机械,2023,39(10):228-232,240.ZHANG G S,SU W W,XU G Y,et al.Progress in the effect of oligosaccharide modification on gelability of egg white protein[J].Food & Machinery,2023,39(10):228-232,240.
[30] 曾冰蕙,郝梦真,刘桂蓉,等.酪蛋白糖基化改性的研究进展[J].食品工业科技,2023,44(6):477-484.DENG B H,HAO M Z,LIU G R,et al.Research progress of casein glycosylation and changes in functional properties [J].Science and Technology of Food Industry,2023,44(6):477-484.
[31] 万俊,张孝芹,邓博文,等.糖基化结合单宁酸改性对熔球态蛋 清 蛋 白 乳 化 特 性 的 影 响 [J].食 品 与 机 械,2024,40(11):12-18.WAN J,ZHANG X Q,DENG B W,et al.Effects of glycosylation combined with tannic acid modification on the emulsification properties of molten globule egg white protein[J].Food & Machinery,2024,40(11):12-18.
[32] AL-HAKKAK J,AL-HAKKAK F.Functional egg white-pectin conjugates prepared by controlled Maillard reaction [J].Journal of Food Engineering,2010,100(1):152-159.
[33] KATO Y,AOKI T,KATO N,et al.Modification of ovalbumin with glucose 6-phosphate by amino-carbonyl reaction improvement of protein heat stability and emulsifying activity[J].Journal of Agricultural and Food Chemistry,1995,43(2):301-305.[34] 胥伟,黄迪,王海滨,等.糖基化反应过程中蛋清蛋白粉的功能性研究 [J].食品科技,2016,41(1):33-36.XU W,HUANG D,WANG H B,et al.Functional properties of egg white powder during glycation [J].Food Science and Technology,2016,41(1):33-36.
[35] 王晨莹.糖基化蛋清蛋白的制备、性质及其在纳米颗粒中的应用 [D].无锡:江南大学,2019:32-35.WANG C Y.The preparation,properties of glycated egg white protein and its application in nanoparticles [D].Wuxi:Jiangnan University,2019:32-35.
[36] BILBROUGH T,PIEMONTESE E,SEITZ O.Dissecting the role of protein phosphorylation:a chemical biology toolbox [J].Chemical Society Reviews,2022,51(13):5 691-5 730.
[37] 张根生,李琪,黄昕钰,等.蛋清蛋白凝胶改性及其在肉制品加工中的应用 [J].食品与机械,2023,39(4):198-204.ZHANG G S,LI Q,HUANG X Y,et al.Egg white protein gel modification and its application in meat processing [J].Food & Machinery,2023,39(4):198-204.
[38] 杨燃,宋洪波,黄群,等.鸡蛋清磷酸化蛋白质组鉴定与分析[J].食品科学,2019,40(11):30-35.YANG R,SONG H B,HUANG Q,et al.Identification and analysis of phosphoproteome in chicken egg white [J].Food Science,2019,40(11):30-35.
[39] LV L,CHI Y J.Improvement of functional properties of ovalbumin phosphorylated by dry-heating in the presence of pyrophosphate [J].European Food Research and Technology,2012,235(5):981-987.
[40] HAYASHI Y,LI C P,ENOMOTO H,et al.Improvement of functional properties of ovotransferrin by phosphorylation through dry-heating in the presence of pyrophosphate [J].Asian-Australasian Journal of Animal Sciences,2008,21(4):596-602.
[41] JAFARI Z,GOLI M,TOGHYANI M.The effects of phosphorylation and microwave treatment on the functional characteristics of freeze-dried egg white powder [J].Foods,2022,11(17):2 711.
[42] 刘潇,吴进菊,高金燕,等.食物蛋白质的酶法改性研究进展[J].食品科学,2010,31(19):409-413.LIU X,WU J J,GAO J Y,et al.Research progresses in enzymatic modification of food proteins [J].Food Science,2010,31(19):409-413.
[43] 熊舟翼,马美湖,卢素芳,等.酶法与非酶法磷酸化改性食品蛋白质的研究进展 [J].食品工业科技,2018,39(21):310-319.XIONG Z Y,MA M H,LU S F,et al.Research progress of enzymatic and non-enzymatic phosphorylation of food proteins[J].Science and Technology of Food Industry,2018,39(21):310-319.
[44] 胡敢.琥珀酰化卵白蛋白凝胶结构及其伤口敷料应用研究[D].武汉:华中农业大学,2022:14.HU G.Study on the structure of succinylated ovalbumin gels and their application in wound dressings [D].Wuhan:Huazhong Agricultural University,2022:14.
[45] GANDHI S K,SCHULTZ J R,BOUGHEY F W,et al.Chemical modification of egg white with 3,3-dimethylglutaric anhydride [J].Journal of Food Science,1968,33(2):163-169.
[46] LAWAL O S.Functionality of native and succinylated Lablab bean (Lablab purpureus ) protein concentrate [J].Food Hydrocolloids,2005,19(1):63-72.
[47] WU Y Y,XIANG X L,LIU L,et al.Ultrasound-assisted succinylation comprehensively improved functional properties of egg white protein [J].LWT-Food Science and Technology,2022,171:114155.
[48] MA C Y,POSTE L M,HOLME J.Effects of chemical modifications on the physicochemical and cake-baking properties of egg white 1[J].Canadian Institute of Food Science and Technology Journal,1986,19(1):17-22.
[49] 姚璇,吕晓慧,金永国,等.动、植物蛋白的酰化改性及其应用研究进展 [J].食品科学,2024,45(16):337-346.YAO X,LÜ X H,JIN Y G,et al.Research progress on acylation modification and application of animal and vegetable proteins [J].Food Science,2024,45(16):337-346.
[50] 文镜,张春华,董雨,等.蛋白质羰基含量与蛋白质氧化损伤[J].食品科学,2003,24(10):153-157.WEN J,ZHANG C H,DONG Y,et al.Review on relationship between protein carbonyl content and protein oxidative injury[J].Food Science,2003,24(10):153-157.
[51] 牛思思.氧化对蛋清蛋白结构及功能特性的影响 [D].天津:天津科技大学,2017:9-11,33-34.NIU S S.Effect of oxidation structure and functional properties of egg white protein [D].Tianjin:Tianjin University of Science & Technology,2017:9-11,33-34.
[52] 牛思思,汪建明,贺雅欣,等.羟基自由基氧化对蛋清蛋白质结构的影响 [J].食品工业科技,2017,38(8):113-117.NIU S S,WANG J M,HE Y X,et al.Effect of hydroxyl radical oxidation system on structure of egg white protein [J].Science and Technology of Food Industry,2017,38(8):113-117.
[53] 李泽坤,肖宇,焦阳,等.脂质和蛋白质氧化对肉品质的影响[J].中国食品学报,2024,24(7):438-449.LI Z K,XIAO Y,JIAO Y,et al.Effects of lipid and protein oxidation on meat quality [J].Journal of Chinese Institute of Food Science and Technology,2024,24(7):438-449.
[54] 龚萍.自由基诱导的氧化对蛋清蛋白结构与功能特性的调节机制探究 [D].长春:吉林大学,2022:9-11.GONG P.Investigation of the regulation mechanism of free radicals-induced oxidation on the structural and functional properties of egg white protein [D].Changchun:Jilin University,2022:9-11.
[55] LI J Y,WANG Z Y,XIAO N,et al.Endogenous reactive oxygen species (ROS )-driven protein oxidation regulates emulsifying and foaming properties of liquid egg white [J].International Journal of Biological Macromolecules,2024,268:131843.
[56] LI S G,HUANG Y,AN F P,et al.Hydroxyl radical-induced early stage oxidation improves the foaming and emulsifying properties of ovalbumin [J].Poultry Science,2019,98(2):1 047-1 054.
[57] 李鹤林,李芳,吴晓娟,等.蛋白质氧化影响乳液稳定性研究进展 [J].中国粮油学报,2021,36(6):190-196.LI H L,LI F,WU X J,et al.Research progress on effects of protein oxidation on emulsion stability [J].Journal of the Chinese Cereals and Oils Association,2021,36(6):190-196.
[58] 李弓中,赵英,王俊彤,等.超声处理对蛋清蛋白结构性质及蛋清液起泡性的影响 [J].食品科学,2019,40(9):68-75.LI G Z,ZHAO Y,WANG J T,et al.Effect of ultrasonic treatment on protein structure and physicochemical and foaming properties of liquid egg white [J].Food Science,2019,40(9):68-75.
[59] 马秋平,王辉,涂宗财,等.超声波辅助糖基化对 β-乳球蛋白消化过程中致敏性的影响 [J].食品与机械,2021,37(4):6-11.MA Q P,WANG H,TU Z C,et al.Effects of ultrasound-assisted glycation on the allergenicity of β-lactoglobulin during digestion [J].Food & Machinery,2021,37(4):6-11.
[60] CHEN W J,MA X B,WANG W J,et al.Preparation of modified whey protein isolate with gum acacia by ultrasound Maillard reaction [J].Food Hydrocolloids,2019,95:298-307.
[61] JIANG M M,CHEN P X,WANG X J,et al.Effect of ultrasonic vacuum drying on the structural characteristics of whole-egg protein powder [J].LWT-Food Science and Technology,2024,191:115490.
[62] ASAITHAMBI N,SINGHA P,SINGH S K.Comparison of the effect of hydrodynamic and acoustic cavitations on functional,rheological and structural properties of egg white proteins [J].Innovative Food Science & Emerging Technologies,2022,82:103166.
[63] ABKER A M,WANG S R,CHEN S H,et al.The effects of high-intensity ultrasound and oil types on the physicochemical properties of egg white protein emulsions [J].Journal of Food Engineering,2023,339:111276.
[64] 邹宇欣,谢静雯,王洪涛,等.超声对动物蛋白结构及性质影响研究进展 [J].食品工业科技,2024,45(9):399-409.ZHOU Y X,XIE J W,WANG H T,et al.Research progress on the effect of ultrasound on animal protein structure and properties [J].Science and Technology of Food Industry,2024,45(9):399-409.
[65] 闫雨洁.超声结合糖基化反应改性卵白蛋白及其理化性质与乳化性能研究 [D].南宁:广西大学,2021:36-45.YAN Y J.Study on the physicochemical and emulsifying properties of ovalbumin modified by ultrasonically assisted glycation [D].Nanning:Guangxi University,2021:36-45.|[66] 谢欢.不同时空条件下微波对蛋清蛋白糖基化反应的影响[D].南昌:南昌大学,2017:4.XIE H.Effect of microwave on glycation reaction of egg white with different space-time conditions [D].Nanchang:Nanchang University,2017:4.
[67] 冯建慧,曹爱玲,陈小强,等.微波对食品蛋白凝胶性和结构影响研究进展 [J].食品工业科技,2017,38(18):317-322.FENG J H,CAO A L,CHEN X Q,et al.Research progress of effect of microwave heating on gelation properties and structure of food protein [J].Science and Technology of Food Industry,2017,38(18):317-322.
[68] 薛 丁 萍,徐 斌,姜 辉,等.食 品 微 波 加 工 中 的 非 热 效 应 研 究[J].中国食品学报,2013,13(4):143-148.XUE D P,XU B,JIANG H,et al.The non-thermal effects of microwave food processing [J].Journal of Chinese Institute of Food Science and Technology,2013,13(4):143-148.
[69] 崔亚鹏,张国治,张康逸.微波处理对食品风味及营养成分影响的研究进展 [J].粮食加工,2020,45(6):62-65.CUI Y P,ZHANG G Z,ZHANG K Y.Research progress on effects of microwave treatment on food flavor and nutritional components [J].Grain Processing,2020,45(6):62-65.
[70] 刘丽莉,史胜娟,吴红梅,等.微波功率对蛋清蛋白粉乳化特性的影响 [J].西北农林科技大学学报 (自然科学版 ),2022,50(5):76-84.LIU L L,SHI S J,WU H M,et al.Effect of microwave power on emulsifying properties of egg white protein powder [J].Journal of Northwest A & F University (Natural Science Edition ),2022,50(5):76-84.
[71] 史胜娟.微波真空冷冻干燥对蛋清蛋白乳化性影响及差异蛋白质组分析 [D].洛阳:河南科技大学,2022:10-15.SHI S J.Effect of microwave vacuum freeze drying on emulsification of egg white protein and differential proteomic analysis [D].Luoyang:Henan University of Science and Technology,2022:10-15.
[72] 陈红兵,杨皞,高金燕,等.微波处理条件对蛋清结构和 IgG结合能力的影响 [J].农业机械学报,2019,50(7):355-361.CHEN H B,YANG H,GAO J Y,et al.Effects of microwave treatment on structure of egg white and IgG binding capacity[J].Transactions of the Chinese Society for Agricultural Machinery,2019,50(7):355-361.
[73] 何大博,仝其根.鸡蛋蛋白质改性研究进展 [J].农产品加工,2018 (3):47-51,54.HE D B,TONG Q G.Advances in modification methods of egg proteins [J].Farm Products Processing,2018 (3):47-51,54.
[74] 钟比真.微波场中卵清蛋白糖基化反应不均匀性的研究 [D].南昌:南昌大学,2021:139-140.ZHONG B Z.Inhomogeneity of glycation of ovalbumin in microwave field [D].Nanchang:Nanchang University,2021:139-140.
[75] JADHAV H B,ANNAPURE U S,DESHMUKH R R.Non-thermal technologies for food processing [J].Frontiers in Nutrition,2021,8:657090.
[76] FERNANDEZ-DIAZ M D,BARSOTTI L,DUMAY E,et al.Effects of pulsed electric fields on ovalbumin solutions and dialyzed egg white [J].Journal of Agricultural and Food Chemistry,2000,48(6):2 332-2 339.
[77] 张铁华,殷涌光,刘静波.高压脉冲电场 (PEF)对蛋清蛋白功能特性的影响 [J].食品科学,2007,28(9):98-102.ZHANG T H,YIN Y G,LIU J B.Effects of high intensity pulsed electric fields on functional properties of egg albumen[J].Food Science,2007,28(9):98-102.
[78] 吴永艳,王恰,段文珊,等.物理改性在蛋清蛋白功能特性改善中的应用 [J].食品与机械,2021,37(3):195-200.WU Y Y,WANG Q,DUAN W S,et al.Application of physical modification in the improvement of functional properties of egg white protein [J].Food & Machinery,2021,37(3):195-200.
[79] 杨宇帆,陈倩,王浩,等.高压电场技术在食品加工中的应用研究进展 [J].食品工业科技,2019,40(19):316-320,325.YANG Y F,CHEN Q,WANG H,et al.Research progress of high voltage electric field technology in food processing [J].Science and Technology of Food Industry,2019,40(19):316-320,325.

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