Abstract
[Objective] To optimize the formula of a chitosan (CS)-carboxymethyl cellulose (CMC) composite film, and to enhance its antimicrobial activity and preservation effect on cherry tomatoes through the incorporation of olive pomace polyphenols (OPP) and curcumin (Cur). [Methods] CS and CMC are used as the matrix, into which OPP and Cur are incorporated. Then, the formula is optimized using response surface methodology, with the inhibition zone diameter against E.coli as the indicator. [Results] The optimal formula is 1.5% CS, 3% CMC, 8% OPP, 3% Cur, 0.9% glycerol, and 1.2% acetic acid, all based on the weight of water. The resulting CS-CMC-OPP-Cur (COPC) film exhibits significantly improved properties. The density is 1.773 g/cm3. The tensile strength is 8.78 MPa, 63.50% higher than that of the chitosan-carboxymethyl cellulose (CM) film. The elongation at break is 29.79%, representing a 26.60% increase over the CM film. The water vapor permeability is 2.38 mg·mm/ (cm2·kPa·d), 42.93% lower than that of the CM film, while the inhibition zone diameter reaches 16.39 mm. In cherry tomato preservation tests over 15 days, the COPC coated group exhibits a decay rate of 46.08%, a weight loss of 2.94%, and a soluble solid rate of 27.18%, significantly outperforming the blank and control groups. The loss rates of titratable acid and vitamin C are 0.32% and 0.318%, respectively, extending the shelf life beyond 15 days. [Conclusion] Through component synergy, the physical properties, barrier performance, and antimicrobial activity of the COPC film are significantly improved, effectively delaying the quality deterioration of cherry tomatoes.
Publication Date
5-13-2026
First Page
111
Last Page
120
DOI
10.13652/j.spjx.1003.5788.2025.80793
Recommended Citation
Longying, NIE; Lijun, JIAO; Jiamin, YANG; Wenbin, YE; and Xiujuan, ZHU
(2026)
"Construction of a quaternary composite antimicrobial film and its preservation-enhancing effect on cherry tomatoes,"
Food and Machinery: Vol. 42:
Iss.
3, Article 15.
DOI: 10.13652/j.spjx.1003.5788.2025.80793
Available at:
https://www.ifoodmm.cn/journal/vol42/iss3/15
References
[1] 刘畅,周舟,陈秋怡,等.茶多酚 /壳聚糖 /百里香精油复合涂膜对圣女果保鲜效果的影响 [J].包装工程,2025,46(3):104-112.LIU C,ZHOU Z,CHEN Q Y,et al.Effect of tea polyphenols/chitosan/thyme oil composite coating on preservation of cherry tomatoes [J].Packaging Engineering,2025,46(3):104-112.
[2] 吴烨.脉冲电场强化大豆分离蛋白—壳聚糖相互作用制备高阻隔性保鲜膜研究 [D].广州:华南理工大学,2024:47.WU Y.Preparation of high-barrier cling film by pulsed electric field-enhanced soybean isolate protein-chitosan interactions [D].Guangzhou:South China University of Technology,2024:47.
[3] 卢嘉敏.绿色可降解食品保鲜材料的研究进展 [J].包装工程,2023,44(S2):77-81.LU J M.Research progress of green degradable food preservation materials [J].Packaging Engineering,2023,44(S2):77-81.
[4] 胡预龙,张如强,李文俊,等.生物可降解食品包装材料的研究进展 [J].精细化工,2025,42(6):1 211-1 220.HU Y L,ZHANG R Q,LI W J,et al.Research progress on biodegradable food packaging materials [J].Fine Chemicals,2025,42(6):1 211-1 220.
[5] 连欢,孙占新,杨相政.壳聚糖抑菌复合膜对蓝莓贮藏保鲜效果的影响 [J].中国果菜,2023,43(9):29-34.LIAN H,SUN Z X,YANG X Z.Effect of antibacterial chitosan composite film on storage and preservation of blueberry [J].China Fruit & Vegetable,2023,43(9):29-34.
[6] FONSECA-GARCÍA A,JIMÉNEZ-REGALADO E J,AGUIRRE-LOREDO R Y.Preparation of a novel biodegradable packaging film based on corn starch-chitosan and poloxamers[J].Carbohydrate Polymers,2021,251:117009.
[7] 项丹丹,项梦丹,孙龙.银负载槲皮素—壳聚糖复合膜的制备及其对草莓的保鲜效果 [J].粮食与油脂,2025,38(9):69-75.XIANG D D,XIANG M D,SUN L.Preparation of silver-loaded quercetin-chitosan composite membrane and its preservation effect on strawberries [J].Cereals & Oils,2025,38(9):69-75,88.
[8] 郭培锋,吴雨威,谢杏婵,等.壳聚糖—大豆分离蛋白复合膜制备工艺优化及对芒果的保鲜效果 [J].食品与机械,2025,41(1):149-157.GUO P F,WU Y W,XIE X C,et al.Optimization of chitosan-soy protein isolate composite film fabrication and its preservation effect on mangoes [J].Food & Machinery,2025,41(1):149-157.
[9] 闫运开,宋见喜,毛迪锐,等.植物多酚—壳聚糖抑菌保鲜膜的研究进展 [J].食品工业科技,2021,42(3):326-331.YAN Y K,SONG J X,MAO D R,et al.Research progress of plant polyphenol-chitosan antibacterial plastic wrap [J].Science and Technology of Food Industry,2021,42(3):326-331.
[10] 洪鹏,蓝凤霞,李剑,等.嘉宝果提取物 /壳聚糖复合膜的制备及应用研究 [J].塑料包装,2024,34(3):36-40,25.HONG P,LAN F X,LI J,et al.Study on the preparation and application of myrciaria cauliflora extract/chitosan composite films [J].Plastics Packaging,2024,34(3):36-40,25.
[11] 李霞,高恒芳,白皙,等.葡萄皮渣多酚—壳聚糖复合可食膜的性能及应用 [J].包装与食品机械,2024,42(2):26-34.LI X,GAO H F,BAI X,et al.Properties and application of grape pomace polyphenol-chitosan composite edible film [J].Packaging and Food Machinery,2024,42(2):26-34.
[12] XU P C,WANG T,HE J,et al.Antibacterial rice protein nanoparticles with a high curcumin loading for fruit preservation [J].Food Bioscience,2024,61:104935.
[13] 庄迪.姜黄素—虫胶纳米颗粒光敏抑菌明胶膜的制备及保鲜机制 [D].咸阳:西北农林科技大学,2024:36.ZHUANG D.Photodynamic-responsive and antibacterial gelatin-based film with curcumin-shellac nanoparticles:study on formation and preservation mechanism [D].Xianyang:Northwest A & F University,2024:36.
[14] GUO Q,SU J Q,SHU X,et al.Production and characterization of pea protein isolate-pectin complexes for delivery of curcumin:effect of esterified degree of pectin [J].Food Hydrocolloids,2020,105:105777.
[15] 李婷,刘蕾,黄敏,等.负载姜黄素的玉米醇溶蛋白—羧甲基壳 聚 糖 纳 米 复 合 物 的 制 备 表 征、体 外 消 化 及 其 抗 氧 化 活 性[J].现代食品科技,2022,38(12):110-117.LI T,LIU L,HUANG M,et al.Preparation,characterization,in vitro digestion,and antioxidant activity of curcumin-loaded zein-carboxymethyl chitosan nano complexes [J].Modern Food Science and Technology,2022,38(12):110-117.
[16] YAMADA N,MATSUSHIMA-NISHIWAKI R,MASUE A,et al.Olive oil polyphenols suppress the TGF- α-induced migration of hepatocellular carcinoma cells [J].Biomedical Reports,2019,1(1):1-5.
[17] 聂龙英,苏满春,焦利军,等.响应面法优化油橄榄果渣多酚提取工艺及其抑菌活性研究 [J].中国饲料,2025 (13):49-56.NIE L Y,SU M C,JIAO L J,et al.Optimization of extraction technology of polyphenols from olive pomace by response surface methodology and its antibacterial activity [J].China Feed,2025 (13):49-56.
[18] MIAO Y X,XU Y,GAO J,et al.Transcriptomic analysis reveals molecular mechanism by which Chinese olive fruit prolongs lifespan of Caenorhabditis elegans [J].NPJ Science of Food,2025,9(1):90.
[19] 尚方园.橄榄叶中多酚物质的提取、分离、纯化及其在核桃油中的应用 [D].郑州:河南工业大学,2022:38.SHANG F Y.Extraction,separation and purification of polyphenols from olive leaves and their application in walnut oil[D].Zhengzhou:Henan University of Technology,2022:38.
[20] 聂龙英,焦利军,卓平清,等.油橄榄果渣多酚基抗氧化膜的制备及其抑菌活性的测定 [J].包装与食品机械,2025,43(4):77-86.NIE L Y,JIAO L J,ZHUO P Q,et al.Preparation of olive pomace polyphenols-based antioxidant film and determination of its antibacterial activity [J].Packaging and Food Machinery,2025,43(4):77-86.
[21] 黄坤,黄世豪,莫璧良,等.枇杷叶提取物与壳聚糖复合膜的制备、表征及其在香蕉保鲜中的应用 [J].食品科技,2023,48(11):198-207.HUANG K,HUANG S H,MO B L,et al.Preparation and characterization of loquat leaf extract and chitosan composite film and its application in banana preservation [J].Food Science and Technology,2023,48(11):198-207.
[22] 刘哲.两种微藻多糖与壳聚糖复合膜的制备、表征及其防腐保鲜功效 [D].晋中:山西农业大学,2021:49.LIU Z.Preparation,characterization and preservative effect of two kinds of microalgae polysaccharide and chitosan composite membranes [D].Jinzhong:Shanxi Agricultural University,2021:49.
[23] 曹 建 康,姜 微 波,赵 玉 梅.果 蔬 采 后 生 理 生 化 实 验 指 导 [M].北京:中国轻工业出版社,2007:18-20.CAO J K,JIANG W B,ZHAO Y M.Guidance for postharvest physiological and biochemical experiments of fruits and vegetables [M].Beijing:China Light Industry Press,2007:18-20.
[24] 徐元柳,刘迪迪,王兆丹,等.植物提取物抑菌机理及联合现代新兴技术在肉类保鲜中的应用进展 [J].食品与机械,2024,40(11):238-244.XU Y L,LIU D D,WANG Z D,et al.Research progress on the antibacterial mechanism of natural plant extracts and its application in meat preservation by combining with modern emerging technologies [J].Food & Machinery,2024,40(11):238-244.
[25] 周阿容.基于姜黄素 /β-环糊精的光敏减菌膜的制备与应用[D].福州:福建农林大学,2020:28.ZHOU A R.Preparation and application of photodynamic antibacterial film based on curcumin/ β-cyclodextrin [D].Fuzhou:Fujian Agriculture and Forestry University,2020:28.
[26] 刘新宇.天然多糖基薄膜在食品保鲜及智能化检测中的应用[D].烟台:烟台大学,2025:33.LIU X Y.Applications of natural polysaccharide-based films in food preservation and smart monitoring [D].Yantai:Yantai University,2025:33.
[27] HAN J,CHEN F L,GAO C C,et al.Environmental stability and curcumin release properties of Pickering emulsion stabilized by chitosan/gum Arabic nanoparticles [J].International Journal of Biological Macromolecules,2020,157:202-211.
[28] LIU Y J,CAI Y X,JIANG X Y,et al.Molecular interactions,characterization and antimicrobial activity of curcumin-chitosan blend films [J].Food Hydrocolloids,2016,52:564-572.
[29] XIAO Y Q,LIU Y N,KANG S F,et al.Development of pH-responsive antioxidant soy protein isolate films incorporated with cellulose nanocrystals and curcumin nanocapsules to monitor shrimp freshness [J].Food Hydrocolloids,2021,120:106893.
[30] 刘端武,廖海周,杨敏,等.聚乙烯醇—果胶—乳清蛋白复合膜 性 能 及 其 在 圣 女 果 保 鲜 中 应 用 的 研 究 [J].食 品 与 发 酵 工业,2025,51(10):119-127.LIU D W,LIAO H Z,YANG M,et al.Properties of polyvinyl alcohol-pectin-whey protein composite film and application in cherry tomato preservation [J].Food and Fermentation Industries,2025,51(10):119-127.
[31] 曲梦锐,刘雨辰,曹恺洋,等.壳聚糖与芫荽精油复合涂膜在圣女果保鲜中的应用研究 [J].中国食品添加剂,2024,35(6):117-122.QU M R,LIU Y C,CAO K Y,et al.Application of composite coating film of chitosan and coriander essential oil in fresh-keeping of cherry tomatoes [J].China Food Additives,2024,35(6):117-122.
