Abstract
[Objective] To systematically evaluate the comprehensive effects of high-pressure processing (HPP) on microbial inactivation, physicochemical properties, and volatile aroma compounds of golden tea beverage, and to optimize a non-thermal processing technique, in response to the negative impact of conventional thermal sterilization on its quality. [Methods] Golden tea beverage was treated under different pressure-time combinations using HPP. Microbial indicators were determined, and microbial inactivation kinetic models were established. Changes in physicochemical properties (tea polyphenols, free amino acids, total soluble sugars, pH, and color) were analyzed, and sensory evaluation was conducted to determine the optimal processing parameters. Meanwhile, volatile aroma compounds before and after HPP were characterized using headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). [Results] The Weibull model more accurately described the non-log-linear inactivation behavior of microorganisms. Under the optimal condition (400 MPa for 5 min), the total viable count decreased to (8±2) CFU/mL, fully meeting national beverage hygiene standards, while achieving the highest overall sensory score and maximizing the retention of color and taste. Aroma analysis showed that HPP reduced certain floral and fresh compounds, such as nonanal (decreased by 86.2%) and linalool (decreased by 65.3%), while increasing ripe-sweet |compounds such as β-myrcene. The aroma profile shifted from a "fresh floral" type to a "ripe-sweet" type, without generating typical heat-induced off-flavors. [Conclusion] HPP (400 MPa for 5 min) effectively inactivated microorganisms in golden tea beverage, better maintained aroma harmony, and avoided flavor distortion, demonstrating its feasibility as a non-thermal processing technology.
Publication Date
5-13-2026
First Page
158
Last Page
166
DOI
10.13652/j.spjx.1003.5788.2026.80015
Recommended Citation
Han, WANG; Maojun, YAO; Yingzi, CAO; Kaikun, LUO; and Yongsheng, TAN
(2026)
"Effects of high-pressure processing on microbial indicators, physicochemical indicators, and aroma components of golden tea beverage,"
Food and Machinery: Vol. 42:
Iss.
3, Article 20.
DOI: 10.13652/j.spjx.1003.5788.2026.80015
Available at:
https://www.ifoodmm.cn/journal/vol42/iss3/20
References
[1] ZHONG N,ZHAO X,YU P H,et al.Characterization of the sensory properties and quality components of Huangjin green tea based on molecular sensory-omics [J].Foods,2023,12(17):3 234.
[2] 欧阳建,黄纯勇,李适,等.黄金茶绿茶风味轮及滋味分类模型的构建 [J].食品与发酵工业,2022,48(24):139-146.OUYANG J,HUANG C Y,LI S,et al.Construction of flavor wheel and taste classification model for Huangjincha green tea[J].Food and Fermentation Industries,2022,48(24):139-146.
[3] SONI A,OEY I,SILCOCK P,et al.Bacillus spores in the food industry:a review on resistance and response to novel inactivation technologies [J].Comprehensive Reviews in Food Science and Food Safety,2016,15(6):1 139-1 148.
[4] ROOBAB U,AADIL R M,MADNI G M,et al.The impact of nonthermal technologies on the microbiological quality of juices:a review [J].Comprehensive Reviews in Food Science and Food Safety,2018,17(2):437-457.
[5] CHEN D,PAN S X,CHEN J,et al.Comparing the effects of high hydrostatic pressure and ultrahigh temperature on quality and shelf life of cloudy ginger juice [J].Food and Bioprocess Technology,2016,9(10):1 779-1 793.
[6] 张园园,刘振民,郑远荣.超高压处理对奶油奶酪质构、风味及货架期的影响 [J].食品与机械,2023,39(8):110-115,174.ZHANG Y Y,LIU Z M,ZHENG Y R.Effect of ultra - high pressure treatment on the texture,flavor and shelf life of cream cheese [J].Food & Machinery,2023,39(8):110-115,174.
[7] PEI L Y,LI J,XU Z L,et al.Effect of high hydrostatic pressure on aroma components,amino acids,and fatty acids of Hami melon (Cucumis melo L.var.reticulatus naud.) juice [J].Food Science & Nutrition,2020,8(3):1 394-1 405.
[8] 邓建,唐政,万睿智,等.藤茶制备关键工艺优化及成品风味特征研究 [J].食品与机械,2025,41(8):155-163.DENG J,TANG Z,WAN R Z,et al.Key process optimization of vine tea and flavor characteristics of finished products [J].Food & Machinery,2025,41(8):155-163.
[9] 安勤,鲍肃都,陈宏宇,等.基于 GC×GC-QTOF-MS 分析不同品种汝城白毛茶白茶的香气特征 [J].食品科学,2025,46(4):163-171.AN Q,BAO S D,CHEN H Y,et al.Analysis of key aroma compounds in three varieties of Rucheng white tea by comprehensive two-dimensional gas chromatography-quadrupole time-of-flight mass spectrometry [J].Food Science,2025,46(4):163-171.
[10] GEERAERD A H,VALDRAMIDIS V P,VAN IMPE J F.GInaFiT,a freeware tool to assess non-log-linear microbial survivor curves [J].International Journal of Food Microbiology,2005,102(1):95-105.
[11] HAYASHI R.High pressure in food processing and preservation:principle,application and development [J].High Pressure Research,1991,7(1/2/3/4/5/6):15-21.
[12] MCKAY A M,LINTON M,STIRLING J,et al.A comparative study of changes in the microbiota of apple juice treated by high hydrostatic pressure (HHP) or high pressure homogenisation (HPH) [J].Food Microbiology,2011,28(8):1 426-1 431.
[13] HU Y W,SUN H N,MU T H.Effects of different pH,temperature,and pressurisation time on the stability of chlorophylls treated with Zn2+ and high hydrostatic pressure [J].International Journal of Food Science & Technology,2023,58(9):4 715-4 725.
[14] CHEN X Q,ZHAO Y L,ZHAO Y,et al.Effect of ultra-high pressure treatment on the characteristics of a tea polysaccharide conjugate aqueous solution [J].Industrial Crops and Products,2021,171:113859.
[15] HUANG H W,LUNG H M,YANG B B,et al.Responses of microorganisms to high hydrostatic pressure processing [J].Food Control,2014,40:250-259.
[16] 侯思涵.超高压对哈密瓜汁中关键酶、氨基酸及理化指标影响的研究 [D].石河子:石河子大学,2018:22.HOU S H.Effects of ultra high pressure on essential enzymes,amino acids and physicochemical index in Hamimelon juice[D].Shihezi:Shihezi University,2018:22.
[17] MARTÍNEZ-MONTEAGUDO S I,BALASUBRAMANIAM V M.Fundamentals and applications of high-pressure processing technology [M]// BALASUBRAMANIAM V,BARBOSA-CÁNOVAS G,LELIEVELD H.High pressure processing of food.New York:Springer,2016:1-17.
[18] SHKOLNIKOV H,BELOCHVOSTOV V,OKUN Z,et al.The effect of pressure on the kinetics of polyphenolics degradation- implications to hyperbaric storage using Epigallocatechin-gallate as a model [J].Innovative Food Science & Emerging Technologies,2020,59:102273.
[19] 焦海珍,李乐,田双红,等.4个黄金茶品种 （系）绿茶和红茶香气品质特征及关键香气成分研究 [J].茶叶科学,2025,45(6):931-942.JIAO H Z,LI L,TIAN S H,et al.Aroma quality characteristics and key aroma components analysis of green and black tea from four 'Huangjincha'-related cultivars [J].Journal of Tea Science,2025,45(6):931-942.
[20] SONG Y C,BI X F,ZHOU M,et al.Effect of combined treatments of ultrasound and high hydrostatic pressure processing on the physicochemical properties,microbial quality and shelf-life of cold brew tea [J].International Journal of Food Science & Technology,2021,56(11):5 977-5 988.
[21] XING L J,ZHANG H,QI R L,et al.Recent advances in the understanding of the health benefits and molecular mechanisms associated with green tea polyphenols [J].Journal of Agricultural and Food Chemistry,2019,67(4):1 029-1 043.
[22] 汪洁琼.绿茶饮料熟闷气味物质鉴定及形成机制与调控技术研究 [D].重庆:西南大学,2024:50.WANG J Q.Identification of substances,formation mechanism and regulation technology of retort odor of green tea beverages [D].Chongqing:Southwest University,2024:50.
[23] TAO M,GUO W L,LIANG J,et al.Unraveling the key cooked off-flavor compounds in thermally sterilized green tea beverages,and masking effect of tea raw material baking [J].Food Chemistry,2025,464:141671.
[24] 王聪明.基于风味组学的四川典型绿茶和红茶香型关键组分筛选及呈香特征评价 [D].成都:四川农业大学,2024:100-101.WANG C M.Selection of key aroma components and evaluation of aroma characteristics of typical green and black teas in Sichuan based on flavor omics [D].Ya'an:Sichuan Agricultural University,2024:100-101.
