•  
  •  
 

Corresponding Author(s)

邢广旭(1979—), 男, 河南省农业科学院研究员, 博士。E-mail: xingguangxu@163.com

Abstract

As a broad-spectrum antibiotic, florfenicol is widely used in livestock, poultry and aquaculture. Due to the unregulated use by some enterprises, a large amount of florfenicol residue exists in animal-derived foods. When florfenicol enters the human body through the food chain, it can cause gastrointestinal discomfort, intoxication, and even aplastic anemia. To minimize the effects of florfenicol residues and derivatives in food on human beings, many countries have set strict limits for maximum florfenicol residues in food. Therefore, this paper summarizes the detection methods for florfenicol established in recent years and gives an outlook on their development trends.

Publication Date

5-13-2026

First Page

189

Last Page

196

DOI

10.13652/j.spjx.1003.5788.2025.80317

References

[1] 龙伟,肖娜,袁丽娟,等.氟苯尼考在泰和乌鸡可食性组织中残留消除规律研究 [J].中国家禽,2024,46(4):80-87.LONG W,XIAO N,YUAN L J,et al.Residue depletion of florfenicol in various edible tissues of taihe black-bone silky fowl [J].China Poultry,2024,46(4):80-87.
[2] 张钰.鸡蛋中氟苯尼考的代谢变化规律研究 [D].烟台:烟台大学,2023:1.ZHANG Y.Study on the metabolic changes of florfenicol in eggs [D].Yantai:Yantai University,2023:1.
[3] TRIF E,CERBU C,OLAH D,et al.Old antibiotics can learn new ways:a systematic review of florfenicol use in veterinary medicine and future perspectives using nanotechnology [J].Animals:an Open Access Journal from MDPI,2023,13(10):1 695.
[4] 管凡荀.鸡蛋、禽肉中氟苯尼考及其代谢物和三种氟喹诺酮类药物残留同时检测的 HPLC-FLD 方法研究 [D].扬州:扬州大学,2023:1.GUAN F X.Simultaneous determination of florfenicol,its metabolite and three fluoroquinolones residues in chicken eggs and poultry muscles by high performance liquid chromatography with fluorescence detection [D].Yangzhou:Yangzhou University,2023:1.
[5] HAN C,WEI Y Y,CUI Y Q,et al.Florfenicol induces oxidative stress and hepatocyte apoptosis in broilers via nrf2 pathway [J].Ecotoxicology and Environmental Safety,2020,191:110239.
[6] 卢春雨,张璐,刘维,等.氟苯尼考通过影响肉鸡药物代谢和介 导 炎 症 反 应 诱 导 肾 损 伤 [J].中 国 兽 医 学 报,2022,42(10):2 051-2 058.LU C Y,ZHANG L,LIU W,et al.Florfenicol induces renal injury by affecting drug metabolism and mediating inflammatory responses in broiler chickens [J].Chinese Journal of Veterinary Science,2022,42(10):2 051-2 058.
[7] 边文文,范芳芳,魏宁果.动物源性食品中氯霉素类药物残留量风险分析 [J].农产品加工,2019 (11):59-61.BIAN W W,FAN F F,WEI N G.Risk analysis of chloramphenicol thiamphenicol and forfenicol residues in animal-origin food [J].Agricultural Products Processing,2019(11):59-61.
[8] 王悦,隋颖.动物源性食品中兽药残留的危害及控制策略 [J].食品界,2021 (7):112.WANG Y,SUI Y.Harm and control strategy of veterinary drug residues in animal-derived foods [J].Food Industry,2021(7):112.
[9] 张彩云,赵兴鑫,东贤,等.浅谈兽药残留对人体的危害 [J].今日畜牧兽医,2021,37(11):1,9.ZHANG C Y,ZHAO X X,DONG X,et al.On the harm of veterinary drug residues to human body [J].Today's Animal Husbandry and Veterinary Medicine,2021,37(11):1,9.
[10] EMA/MRL/ 822/02-FINAL January 2002 [DB/OL ].[2024 -10-11].https://www.ema.europa.eu/en/documents/mrl-report/florfenicol-extension-all-food-producing-species-summary-report-6-committee-veterinary-medicinal-en.pdf.
[11] Code of Federal Regulations [DB/OL ].[2024 -10-11].https://www.ecfr.gov/current/title- 21/chapter-I/subchapter-E/part- 556/subpart-B/section- 556.283.
[12] 中华人民共和国农业农村部,中华人民共和国国家健康委员会,国家市场监督管理总局.食品安全国家标准 食品中兽药最大残留限量:GB 31650 —2019 [S].北京：中国标准出版社,2019.Ministry of Agriculture and Rural Affairs of the People's Republic of China,National Health Commission of the People's Republic of China,State Administration for Market Regulation.National food safety standard-Maximum residue limits for veterinary drugs in foods:GB 31650 —2019 [S].Beijing:China Standards Press,2019.
[13] WANG B,XIE X,ZHAO X,et al.Development of an accelerated solvent extraction-ultra-performance liquid chromatography-fluorescence detection method for quantitative analysis of thiamphenicol,florfenicol and florfenicol amine in poultry eggs [J].Molecules (Basel,Switzerland),2019,24(9):1 830.
[14] WANG B,PANG M D,ZHAO X,et al.Development and comparison of liquid-liquid extraction and accelerated solvent extraction methods for quantitative analysis of chloramphenicol,thiamphenicol,florfenicol,and florfenicol amine in poultry eggs [J].Journal of Mass Spectrometry:JMS,2019,54(6):488-494.
[15] HONG L,LIU Y,GUO Y W,et al.Simultaneous determination of florfenicol,its metabolite and three fluoroquinolone residues in poultry muscles via high-performance liquid chromatography with fluorescence detection [J].Microchemical Journal,2024,207:111754.
[16] XIE K Z,JIA L F,YAO Y L,et al.Simultaneous determination of thiamphenicol,florfenicol and florfenicol amine in eggs by reversed-phase high-performance liquid chromatography with fluorescence detection [J].Journal of Chromatography B,Analytical Technologies in the Biomedical and Life Sciences,2011,879(23):2 351-2 354.
[17] LI X,ZHANG Y,SUN Q F,et al.Determination of extractable and non-extractable florfenicol residues as florfenicol amine in eggs by UPLC-MS/MS [J].Food Additives & Contaminants Part A,Chemistry,Analysis,Control,Exposure & Risk Assessment,2022,39(9):1 512-1 520.
[18] VEACH B T,ANGLIN R,MUDALIGE T K,et al.Quantitation and confirmation of chloramphenicol,florfenicol,and nitrofuran metabolites in honey using LC-MS/MS [J].Journal of AOAC International,2018,101(3):897-904.
[19] 许峰,刘倩.Discovery®DSC- 18净化填料—超高效液相色谱串 联 质 谱 法 测 定 蜂 蜜 中 氟 苯 尼 考 和 氟 苯 尼 考 胺 [J].粮 食 与油脂,2021,34(7):157-159,162.XU F,LIU Q.Determination of florfenicol and florfenicol amine in honey by ultra performance liquid chromatography tandem mass spectrometry with Discovery®DSC- 18 purification packing [J].Cereals & Oils,2021,34(7):157-159,162.
[20] WU X Y,SHEN X X,CAO X Y,et al.Simultaneous determination of amphenicols and metabolites in animal-derived foods using ultrahigh-performance liquid chromatography-tandem mass spectrometry [J].International Journal of Analytical Chemistry,2021,2 021:3613670.
[21] 郑陆红,张慧琼,陈茹,等.超高效液相色谱—串联质谱法同时测定动物组织中氯霉素、甲砜霉素、氟苯尼考和其代谢产|物氟苯尼考胺残留量 [J].现代食品,2022,28(20):198-203.ZHENG L H,ZHANG H Q,CHEN R,et al.Determination of chloramphenicol,thiamphenicol,florfenicol and its metabolite florfenicol amine residues in animal tissues by UHPLC-MS/MS[J].Modern Food,2022,28(20):198-203.
[22] 杨秋红,艾晓辉,李荣,等.固相萃取—气相色谱法同时检测水 产 品 中 的 氯 霉 素、甲 砜 霉 素、氟 苯 尼 考 和 氟 苯 尼 考 胺 [J].分析试验室,2015,34(5):533-537.YANG Q H,AI X H,LI R,et al.Simultaneous determination of chloramphenicol,thiamphnicol,florfenicol and forfenicol-amine in aquatic products by Gas chromatographic method with solid phase extraction [J].Chinese Journal of Analysis Laboratory,2015,34(5):533-537.
[23] 郑炜玲.气相色谱法测定草鱼中氯霉素、甲砜霉素、氟苯尼考与氟苯尼考胺残留量 [J].食品安全导刊,2024 (36):28-30.ZHENG W L.Determination of chloramphenicol,thiamphenicol,florfenicol,and florfenicol amine residues in grass carps by gas chromatography [J].China Food Safety Magazine,2024 (36):28-30.
[24] 胡红美,郭远明,雷科,等.分散固相萃取净化—气相色谱法测定水产品中氯霉素和氟苯尼考 [J].食品科学,2014,35(8):231-235.HU H M,GUO Y M,LEI K,et al.Determination of chloramphenicol and florfenicol in fishery products by using dispersive solid phase extraction and gas chromatography [J].Food Science,2014,35(8):231-235.
[25] 张 丽 萍,孟 蕾,张 盼 盼,等.鸡、猪 组 织 中 甲 砜 霉 素、氟 苯 尼考、氟苯尼考胺残留量测定的 GC-MS 法建立 [J].中国兽药杂志,2020,54(9):33-40.ZHANG L P,MENG L,ZHANG P P,et al.Determination of thiamphenicol,florfenicol and florfenicol amine residues in chicken and swine tissues by gas chromatography-mass spectrometry [J].Chinese Journal of Veterinary Drug,2020,54(9):33-40.
[26] 杜春艳,吴发旺,赖淼菊,等.QuEChERS- 超高效液相色谱—串联三重四极杆质谱联用法快速测定羊肉中氯霉素、甲砜霉素、氟苯尼考的残留量 [J].食品与药品,2024,26(3):199-205.DU C Y,WU F W,LAI M J,et al.Rapid determination of chloramphenicol,thiamphenicol and florfenicol residues in mutton by QuEChERS-UPLC-MS/MS [J].Food and Drug,2024,26(3):199-205.
[27] 胡 骁 飞,邢 云 瑞,孙 亚 宁,等.腐 霉 利 人 工 抗 原 制 备 研 究 [J].中国农学通报,2021,37(33):142-147.HU X F,XING Y R,SUN Y N,et al.Research on preparation of artificial antigen of procymidone [J].Chinese Agricultural Science Bulletin,2021,37(33):142-147.
[28] 曹金博,王耀,胡骁飞,等.免疫分析技术在四环素类抗生素残留检测中的应用 [J].饲料工业,2019,40(12):53-59.CAO J B,WANG Y,HU X F,et al.The application of immunoassay technique in the detection of tetracycline antibiotics residue [J].Feed Industry,2019,40(12):53-59.
[29] 孙法良,刁有祥,孙宁,等.鸡肉中氟苯尼考 ELISA 检测方法的建立及应用 [J].中国农业科学,2009,42(5):1 813-1 819.SUN F L,DIAO Y X,SUN N,et al.Establishment and application of detecting the residues of florfenicol in chicken muscle by indirect competitive enzyme-linked immunosorbent assay [J].Scientia Agricultura Sinica,2009,42(5):1 813-1 819.
[30] 孙法良.氟苯尼考、安普霉素药物残留的 ELISA 检测方法研究[D].泰安:山东农业大学,2010:44-59.SUN F L.The study on the establishment of ci-ELISA for the detection of florfenicol and apramycin residue [D].Taian:Shandong Agricultural University,2009:44-59.
[31] 杨典原.氟苯尼考残留酶免疫法的研究 [D].太原:山西大学,2017:23-34.YANG D Y.Study of enzyme immunoassay for florfenicol residues [D].Taiyuan:Shanxi University,2017:23-34.
[32] 谢美婵,杨金易,李然,等.畜禽肉及饲料中氟苯尼考残留免疫分析方法研究 [J].分析测试学报,2021,40(9):1 380-1 385.XIE M C,YANG J Y,LI R,et al.Study on a monoclonal antibody-based immunoassay method for florfenicol in animal meat and feed [J].Journal of Instrumental Analysis,2021,40(9):1 380-1 385.
[33] 廖 文 彬.鸡 肉 中 氟 苯 尼 考 残 留 的 ELISA 检 测 方 法 研 究 [D].广州:华南农业大学,2020:18-38.LIAO W B.The study on the establishment of ELISA for the detection of florfenicol residue in chicken [D].Guangzhou:South China Agricultural University,2020:18-38.
[34] WANG Z F,LUO J Q,ZHAO Y F,et al.Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and florfenicol amine in eggs [J].Food and Agricultural Immunology,2020,31(1):864-875.
[35] GE M,XING Y,SUN Y,et al.Establishment of a high-sensitivity indirect competitive ELISA for florfenicol in chicken meat and egg [J].Acta Alimentaria,2025,54(2):205-215.
[36] GUO L L,SONG S S,LIU L Q,et al.Comparsion of an immunochromatographic strip with elisa for simultaneous detection of thiamphenicol,florfenicol and chloramphenicol in food samples [J].Biomedical Chromatography:BMC,2015,29(9):1 432-1 439.
[37] XIN C,ZHOU J M,CHEN Y M,et al.A novel fluorescence immunoassay for the quantitative detection of florfenicol in animal-derived foods based on ZnCdSe/ZnS quantum dot labelled antibody [J].Food Chemistry,2024,457:139648.
[38] ZENG D P,ZHANG Y Y,YANG J Y,et al.Development of a monoclonal antibody-based time-resolved fluorescence immunochromatographic assay strip for sensitively detecting florfenicol residues in milk and eggs:theoretical chemical insights into unexpected high specificity [J].International Journal of Biological Macromolecules,2024,270:132381.
[39] PENG C,LIANG J F,JIANG L F,et al.Carboxylated fluorescent microsphere based immunochromatographic test strip enabled sensitive and quantitative on-site detection for florfenicol in eggs [J].Journal of Pharmaceutical and Biomedical Analysis,2022,219:114946.
[40] ZHANG E H,LIU B C,LU J H,et al.A helper antibody-based competitive fluorescence immunochromatographic assay for quantitative detection of florfenicol in poultry eggs [J].Journal of AOAC International,2023,106(4):837-845.
[41] TAO X Q,JIANG H Y,YU X Z,et al.Simultaneous determination of chloramphenicol,florfenicol and florfenicol amine in ham sausage with a hybrid chemiluminescent immunoassay [J].Food Additives & Contaminants:Part A,2013,30(5):804-812.
[42] TAO X Q,YU X Z,ZHANG D D,et al.Development of a rapid chemiluminescent ciELISA for simultaneous determination of florfenicol and its metabolite florfenicol amine in animal meat products [J].Journal of the Science of Food and Agriculture,2014,94(2):301-307.
[43] GE M J,XING Y R,SUN Y N,et al.Establishment of a chemiluminescent elisa method for florfenicol in eggs and chicken meat [J].Food Analytical Methods,2024,18:48-56.
[44] SADEGHI A S,MOHSENZADEH M,ABNOUS K,et al.Development and characterization of DNA aptamers against florfenicol:fabrication of a sensitive fluorescent aptasensor for specific detection of florfenicol in milk [J].Talanta,2018,182:193-201.
[45] SHI M H,LIU R B,ZHANG F Y,et al.Screening of single-stranded dna aptamer specific for florfenicol and application in detection of food safety [J].Biosensors,2022,12(9):701.
[46] THOMPSON C S,TRAYNOR I M,FODEY T L,et al.Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor [J].Food Additives & Contaminants Part A,Chemistry,Analysis,Control Exposure & Risk Assessment,2020,37(11):1 854-1 864.
[47] LI Y,ZHOU Y N,PENG Y P,et al.Rapid detection of multiple antibiotics in chicken samples via a fluorescence nanobiosensor coupled with a homemade fluorescence analyzer[J].Analytical Methods:Advancing Methods and Applications,2023,15(27):3 362-3 372.
[48] CARO N,BRUNA T,GUERREIRO A,et al.Florfenicol binding to molecularly imprinted polymer nanoparticles in model and real samples [J].Nanomaterials (Basel,Switzerland),2020,10(2):306.
[49] YUAN Y,ZHU C Z,HANG Q,et al.Hydrophilic molecularly imprinted membranes based on go-loading for simultaneously selective recognition and detection of three amphenicols drugs in pork and milk [J].Food Chemistry,2022,384:132542.
[50] ZHANG X Y,BAI Y C,TANG Q Q,et al.Development of epitopephore-based rational hapten design strategy:a combination of theoretical evidence and experimental validation [J].Journal of Hazardous Materials,2023,445:130615.
[51] YANG Z W,ZHU A F,ALI S,et al.Quantitative detection of chloramphenicol residue in fish based on SERS aptasensor coupled with magnetic separation [J].Microchemical Journal,2024,197:109840.
[52] CHEN H J,CHENG Y J,LIANG Y F,et al.Monoclonal antibody production and quantum dots lateral flow immunoassay for florfenicol detection [J].Journal of Food Composition and Analysis,2024,128:106059.

Download

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.