Abstract
[Objective] To establish a method for the detection of 12 synthetic pigments in wine by solid-phase extraction/ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). [Methods] After the wine is subjected to purification and enrichment via solid-phase extraction, 12 synthetic pigments are determined using ultra-high performance liquid chromatography for separation and mass spectrometry in multiple reaction monitoring (MRM) mode. The influence of matrix effect on the determination is corrected using a matrix-matched calibration curve. [Results] For the 12 synthetic pigments within the range of 0.2~20 000 μg/L, the peak area has a good linear relationship with their mass concentration, and the coefficient of determination R2≥0.982 0.The recovery rates for the analytes at different spiked levels range from 90.8% to 105. 1%, with relative standard deviations (RSDs) of 2. 1%~4.9%. The limits of detection (LODs) of the method are 0.006~1. 500 µg/kg, and the limits of quantitation (LOQs) are 0.02~5.00 µg/kg. The method is used to detect 10 batches of wine samples of different types, of which 5 samples are found to contain acid yellow 36 and indigo carmine. [Conclusion] It is simple, highly sensitive, and offers a wide linear range, making it suitable for the routine determination of the 12 synthetic pigments in wine.
Publication Date
5-13-2026
First Page
49
Last Page
56
DOI
10.13652/j.spjx.1003.5788.2024.80427
Recommended Citation
Xin, LI; Bangrui, LI; Dongyang, CHEN; Dong, ZENG; and Jiaxin, ZUO
(2026)
"Determination of 12 synthetic pigments in wine by solid phase extraction with ultra-high performance liquid chromatography-tandem mass spectrometry,"
Food and Machinery: Vol. 42:
Iss.
3, Article 7.
DOI: 10.13652/j.spjx.1003.5788.2024.80427
Available at:
https://www.ifoodmm.cn/journal/vol42/iss3/7
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