Abstract
[Objective] Microbial strains with tannin-degrading capabilities were screened from natural samples, and their probiotic properties were systematically evaluated. [Methods] Three Lactobacillus plantarum strains (Y1, Y20, Y22) and Meyerozyma caribbica Y3 exhibiting high tanninase activity are selected. Probiotic characteristics including growth characteristics, antioxidant capacity, bile salt tolerance, and cholesterol degradation efficiency are investigated. Safety profiles were determined through hemolytic activity assays and standard antibiotic susceptibility testing. [Results] The experimental data reveal that M.caribbica Y3 demonstrates remarkable antioxidant activities, with a superoxide anion scavenging rate of 95.25 % in cell supernatants and a hydroxyl radical scavenging rate of 63.43 % in extracts after cell wall disruption. Furthermore, its DPPH radical scavenging rate significantly surpasses the co-evaluated L.plantarum Strains. Within the environment of bile salts, this strain maintains a maximal survival rate of 158.82 % and degrades 46.52 % of the cholesterol, and it does not exhibit hemolytic activity. The antibiotic susceptibility test indicates that it has inherent resistance to conventional bacterial antibiotics. [Conclusion] These findings suggest that M.caribbica Y3 holds promise as a candidate for functional fermented products with probiotic effects due to its exceptional antioxidative capacity, gastrointestinal adaptability, and biosafety.
Publication Date
5-15-2026
First Page
33
Last Page
42
DOI
10.13652/j.spjx.1003.5788.2025.80522
Recommended Citation
Wenjing, DUAN; Chong, YU; Yuan, TIAN; Shumei, ZHANG; and Ting, SU
(2026)
"Screening and identification of tannin-degrading strains and analysis of their probiotic functional characteristics,"
Food and Machinery: Vol. 42:
Iss.
4, Article 5.
DOI: 10.13652/j.spjx.1003.5788.2025.80522
Available at:
https://www.ifoodmm.cn/journal/vol42/iss4/5
References
[1] ZENG X Q,JIANG W B,DU Z J,et al.Encapsulation of tannins and tannin-rich plant extracts by complex coacervation to improve their physicochemical properties and biological activities:a review [J].Critical Reviews in Food Science and Nutrition,2023,63(18):3 005-3 018.
[2] ZHANG L L,GUAN Q H,JIANG J C,et al.Tannin complexation with metal ions and its implication on human health,environment and industry:an overview [J].International Journal of Biological Macromolecules,2023,253:127485.
[3] AHMED A I,ABOU-TALEB K A A,ABD-ELHALIM B T.Characterization and application of tannase and gallic acid produced by co-fungi of Aspergillus niger and Trichoderma viride utilizing agro-residues substrates [J].Scientific Reports,2023,13(1):16755.
[4] BORAH A,SELVARAJ S,MURTY V R.Production of gallic acid from Swietenia macrophylla using tannase from Bacillus gottheilii M2S2 in semi-solid state fermentation [J].Waste and Biomass Valorization,2023,14(8):2 569-2 587.
[5] LIANG S,WANG F,CHEN J X,et al.Optimization of a tannase-assisted process for obtaining teas rich in theaflavins from Camellia sinensis leaves [J].Food Chemistry:X,2022,13:100203.
[6] CHAIKAEW S,BAIPONG S,SONE T,et al.Diversity of lactic acid bacteria from Miang,a traditional fermented tea leaf in northern Thailand and their tannin-tolerant ability in tea extract[J].Journal of Microbiology,2017,55(9):720-729.
[7] UNBAN K,KOCHASEE P,SHETTY K,et al.Tannin-tolerant and extracellular tannase producing Bacillus isolated from traditional fermented tea leaves and their probiotic functional properties [J].Foods,2020,9(4):490.
[8] 谭川川,何劲,魏雨萌,等.一株耐单宁酵母菌的筛选、鉴定及发酵特性 [J].食品与发酵工业,2023,49(15):47-53.TAN C C,HE J,WEI Y M,et al.Screening and identification of a tannin-tolerant yeast strain and its fermentation characteristics[J].Food and Fermentation Industries,2023,49(15):47-53.
[9] 阿热爱·巴合提,谭春明,李平兰.益生菌的分类及其多领域应用研究现状 [J].生物加工过程,2022,20(1):88-94.AREAI B,TAN C M,LI P L.Classification and applications of probiotics [J].Chinese Journal of Bioprocess Engineering,2022,20(1):88-94.
[10] 高浩,陈志豪,陆致成,等.植物乳植杆菌 CCFM 8661 和动物双歧杆菌乳亚种 XLTG 11复合益生菌对免疫系统的增强作用[J].中国乳品工业,2024,52(11):13-19.GAO H,CHEN Z H,LU Z C,et al.Lactiplantibacillus plantarum CCFM 8661 and Bifidobacterium animalis subsp.lactis XLTG 11 combined probiotics enhance immune system function [J].China Dairy Industry,2024,52(11):13-19.
[11] STANISZEWSKI A,KORDOWSKA-WIATER M.Probiotic and potentially probiotic yeasts-characteristics and food application [J].Foods,2021,10(6):1 306.
[12] 马子琳.产单宁酶乳酸菌在豆类酸面团馒头中的应用研究[D].无锡:江南大学,2021:13-14.MA Z L.Study on the application of tannase-producing LAB in legumes sourdough steamed bread [D].Wuxi:Jiangnan University,2021:13-14.
[13] 保玉心,邱树毅,李秧针,等.一种胞外单宁酶的活力检测方法[J].精细化工,2008,25(6):621-624.BAO Y X,QIU S Y,LI Y Z,et al.A method for detecting activity of extracellular tannase [J].Fine Chemicals,2008,25(6):621-624.
[14] 张艳芳,吕欣然,武文玉,等.水产品肠道中富硒乳酸菌的筛选 及 其 体 外 生 物 活 性 [J].食 品 研 究 与 开 发,2024,45(18):106-113.ZHANG Y F,LÜ X R,WU W Y,et al.Screening of selenium-rich lactic acid bacteria from intestinal tract of aquatic products and evaluation of its bioactivities in vitro [J].Food Research and Development,2024,45(18):106-113.
[15] ZHANG H,XU J H,CHEN Q,et al.Physiological,morphological and antioxidant responses of Pediococcus pentosaceus R1 and Lactobacillus fermentum R6 isolated from Harbin dry sausages to oxidative stress [J].Foods,2021,10(6):1 203.
[16] 许凤,陈金玲,张梦雪,等.一株发酵条斑紫菜植物乳杆菌的筛选、发酵特性及其发酵多糖抗氧化活性 [J].江苏海洋大学学报,2025,34(1):16-23.XU F,CHEN J L,ZHANG M X,et al.Screening and fermentation characteristics of a strain of fermented Porphyra yezoensis lactobacillus and the antioxidant activity of its fermented polysaccharides [J].Journal of Jiangsu Ocean University,2025,34(1):16-23.
[17] 徐冬美,刘永乐,李向红,等.红曲鲊鱼中原生乳酸菌菌株筛选及发酵工艺优化 [J].食品与机械,2023,39(8):175-185.XU D M,LIU Y L,LI X H,et al.Screening of autochthonouslactic acid bacteria and optimization of fermentation process in low-salt Zhayu of red rice [J].Food & Machinery,2023,39(8):175-185.
[18] 余萍,矫艳平,张春宇,等.具有降胆固醇功能的罗伊氏乳杆菌 的 筛 选 及 其 益 生 作 用 [J].现 代 食 品 科 技,2021,37(11):106-113.YU P,JIAO Y P,ZHANG C Y,et al.Screening for cholesterol-lowering Lactobacillus reuteri strains and analyzing their probiotic effectiveness [J].Modern Food Science and Technology,2021,37(11):106-113.
[19] 王睿敏,郑冬艳,但霞,等.具有潜在降压功能益生乳酸菌的筛选及其特性的研究 [J].食品科技,2025,50(1):1-8.WANG R M,ZHENG D Y,DAN X,et al.Screening and characterization of probiotic lactic acid bacteria with potential antihypertensive function [J].Food Science and Technology,2025,50(1):1-8.
[20] 陈靖,周佳豪,毛琪琪,等.产胞外多糖乳酸菌的筛选及鉴定[J].食品与机械,2023,39(4):26-31,169.CHEN J,ZHOU J H,MAO Q Q,et al.Screening and identification of extracellular polysaccharide-producing lactic acid bacteria [J].Food & Machinery,2023,39(4):26-31,169.
[21] CHEN Y,LI F R,NIELSEN J.Genome-scale modeling of yeast metabolism:retrospectives and perspectives [J].FEMS Yeast Research,2022,22(1):foac 003.
[22] TANG Z H,SHI L Y,LIANG S,et al.Recent advances of tannase:production,characterization,purification,and application in the tea industry [J].Foods,2024,14(1):79.
[23] KHAN N,SINGH A K,SANEJA A.Preparation,characterization,and antioxidant activity of L-ascorbic acid/HP-β-cyclodextrin inclusion complex-incorporated electrospun nanofibers [J].Foods,2023,12(7):1 363.
[24] KANPIENGJAI A,KODCHASEE P,UNBAN K,et al.Three new yeast species from flowers of Camellia sinensis var.assamica collected in Northern Thailand and their tannin tolerance characterization [J].Frontiers in Microbiology,2023,14:1043430.
[25] WU C Y,ZHANG H X,YANG N N,et al.Antioxidant dipeptides enhance osmotic stress tolerance by regulating the yeast cell wall and membrane [J].Journal of Agricultural and Food Chemistry,2024,72(8):4 339-4 347.
[26] OLIVEIRA G S,FREIRE H P S,ROMANO C C,et al.Bioprotective potential of lactic acid bacteria and their metabolites against enterotoxigenic Escherichia coli [J].Microbiology,2022,168(7):001216.
[27] LI W,CHEN H,TANG J G.Interplay between bile acids and intestinal microbiota:regulatory mechanisms and therapeutic potential for infections [J].Pathogens,2024,13(8):702.
[28] MATEUS C,MAIA C J,DOMINGUES F,et al.Evaluation of bile salts on the survival and modulation of virulence of Aliarcobacter butzleri [J].Antibiotics,2023,12(9):1 387.
[29] 王祎然,韦明明,张涵,等.酸汤中乳酸菌的鉴定及其耐酸、耐 胆 盐 和 抗 氧 化 活 性 [J].食 品 工 业 科 技,2020,41(16):121-126,139.WANG Y R,WEI M M,ZHANG H,et al.Identification,acid and bile salt tolerance,and antioxidant ability of lactic acid bacteria isolated from sour soup [J].Science and Technology of Food Industry,2020,41(16):121-126,139.
[30] 于晨,杨露,管峰,等.抑制副溶血性弧菌的乳酸菌筛选鉴定及其生物学特性研究 [J].食品与机械,2023,39(6):12-18.YU C,YANG L,GUAN F,et al.Screening,identification and biological characteristics of lactic acid bacteria inhibiting Vibrio parahaemolyticus [J].Food & Machinery,2023,39(6):12-18.
[31] SUI Y,LIU J T,LIU Y X,et al.In vitro probiotic characterization of Lactobacillus strains from fermented tangerine vinegar and their cholesterol degradation activity [J].Food Bioscience,2021,39:100843.
[32] FERNÁNDEZ-CALDERÓN M C,SÁNCHEZ-MORO M D H,RINCÓN E O.In vitro cholesterol assimilation by Bifidobacterium animalis subsp.lactis (BPL 1) probiotic bacteria under intestinal conditions [J].Endocrine,Metabolic & Immune Disorders Drug Targets,2022,22(4):433-439.
[33] 黄姚,么乃全,袁伟涛,等.鹿源藿香假丝酵母菌 DZ018的分离 鉴 定 及 其 体 外 益 生 特 性 研 究 [J].中 国 畜 牧 兽 医,2025,52(1):389-399.HUANG Y,MO N Q,YUAN W T,et al.Isolation and identification of Candida rugosa DZ 018 from deer and its probiotic properties in vitro [J].China Animal Husbandry & Veterinary Medicine,2025,52(1):389-399.
[34] LEW L C,CHOI S B,KHOO B Y,et al.Lactobacillus plantarum DR 7 reduces cholesterol via phosphorylation of AMPK that down-regulated the mRNA expression of HMG-CoA reductase [J].Korean Journal for Food Science of Animal Resources,2018,38(2):350-361.
[35] LIU Y,ZHENG S J,CUI J L,et al.Effect of bile salt hydrolase-active Lactobacillus plantarum Y15 on high cholesterol diet induced hypercholesterolemic mice [J].CyTA - Journal of Food,2021,19(1):408-417.
[36] 瞿桂花,朱秋劲,万婧.抑制火腿中黑曲霉生长及其合成赭曲霉毒素 A菌株的筛选、鉴定及生长特性 [J].食品工业科技,2024,45(22):107-115.QU G H,ZHU Q J,WAN J.Screening,identification and growth characterization of strains inhibiting the growth and synthesis of ochratoxin A by Aspergillus niger in hams [J].Science and Technology of Food Industry,2024,45(22):107-115.
[37] 冯东华,郑钟立,林勇平,等.益生菌对 16种抗生素的耐药性分析 [J].中国微生态学杂志,2021,33(10):1 176-1 180.FENG D H,ZHENG Z L,LIN Y P,et al.The drug resistance of probiotics to 16 antibiotics [J].Chinese Journal of Microecology,2021,33(10):1 176-1 180.
[38] SIRICHOAT A,FLÓREZ A B,VÁZQUEZ L,et al.Antibiotic susceptibility profiles of lactic acid bacteria from the human vagina and genetic basis of acquired resistances [J].International Journal of Molecular Sciences,2020,21(7):2 594.
[39] STIRKE A,CELIESIUTE-GERMANIENE R,ZIMKUS A,et al.The link between yeast cell wall porosity and plasma membrane permeability after PEF treatment [J].Scientific Reports,2019,9(1):14731.
