•  
  •  
 

Corresponding Author(s)

石萌(1990—),女,湖南农业大学副教授,博士。E-mail:shimeng@hunau.edu.cn

Abstract

[Objective] To construct theaflavins (TFs)-bovine serum albumin (BSA ) complex-based Pickering emulsions to enhance the multiple stability of Pickering emulsions.[Methods] TFs at different mass concentrations (0~20 mg/mL ) were used to prepare complexes with BSA, which were then employed to prepare Pickering emulsions with rapeseed oil. The microstructure, particle size, Zeta potential, and rheological properties of the emulsions were characterized. The effects of TFs mass concentration on the stability of the emulsions under centrifugation, storage, and sodium ion stress were further analyzed.[Results] TFs formed stable complexes with BSA, and the resulting Pickering emulsions exhibited spherical droplets. As the TFs mass concentration increased, the particle size of the emulsions first decreased and then increased. At a TFs mass concentration of 10 mg/mL, the emulsion showed the smallest particle size (D4,3=34.4 μm) and the highest absolute Zeta potential (34.96 mV ), and formed the strongest three-dimensional gel network. Stability analysis revealed that the emulsion at a TFs mass concentration of 10 mg/mL exhibited good water-holding capacity (59.44%), the highest sodium ion stability, and good low -temperature storage stability (4 ℃,20 d).[Conclusion] At a TFs mass concentration of 10 mg/mL, the complexes formed through interactions between TFs and BSA can effectively improve the multiple stability of Pickering emulsions.

Publication Date

6-17-2026

First Page

32

Last Page

41

DOI

10.13652/j.spjx.1003.5788.2026.80153

References

[1] 李丽华,周玉璠.世界红茶发展史略初探 [J].福建茶叶,2019,41(4):215-217.LI L H,ZHOU Y F.A preliminary exploration of the development history of black tea in the world [J].Tea in Fujian,2019,41(4):215-217.
[2] BAJPAI S K,BAJPAI M,SHAH F F.Alginate dialdehyde (AD)-crosslinked casein films:synthesis,characterization and water absorption behavior [J].Designed Monomers and Polymers,2016,19(5):406-419.
[3] BAJPAI S K,SHAH F F,BAJPAI M.Dynamic release of gentamicin sulfate (GS) from alginate dialdehyde (AD)-crosslinked casein (CAS ) films for antimicrobial applications[J].Designed Monomers and Polymers,2017,20(1):18-32.
[4] 刘逸佳,杨涛,傅志轩,等.表没食子儿茶素没食子酸酯—牛血清白蛋白—高酯果胶 Pickering 乳液构建和稳定性差异研究[J].食品与发酵工业,2025,51(14):265-272.LIU Y J,YANG T,FU Z X,et al.Stability of epigallocatechin gallate-bovine serum albumin-high-esterified pectin Pickering emulsion [J].Food and Fermentation Industries,2025,51(14):265-272.
[5] 余振宇.OSA改性芋头淀粉基 Pickering 乳液运载体系稳定机制及其特性研究 [D].合肥:合肥工业大学,2020:43-44.YU Z Y.Stabilization mechanism and characteristics of Pickering emulsion delivery system based on OSA modified taro starch [D].Hefei:Hefei University of Technology,2020:43-44.
[6] 王浩宇,张舒,冯玉超,等.双蛋白纳米颗粒 Pickering 乳液的构建及稳定性研究 [J].中国粮油学报,2024,39(3):98-109.WANG H Y,ZHANG S,FENG Y C,et al.Construction and stability study of Pickering emulsion with dual protein nanoparticles [J].Journal of the Chinese Cereals and Oils Association,2024,39(3):98-109.
[7] 胡杏梅.基于牛血清白蛋白与席夫碱的组合而构建多输出分子逻辑电路 [D].漳州:闽南师范大学,2023:27.HU X M.Construction of multi-output molecular logic circuit based on the combination of bovine serum albumin and schiff base [D].Zhangzhou:Minnan Normal University,2023:27.
[8] 蔡鸿辉.荧光探针的合成及其对金属离子和牛血清白蛋白的检测研究 [D].南宁:广西大学,2020:19.CAI H H.Research on the synthesis of fluorescent probe and its detection of metal ions and bovine serum albumin [D].Nanning:Guangxi University,2020:19.
[9] TABASSUM S,AL-ASBAHY W M,AFZAL M,et al.Synthesis,characterization and interaction studies of copper based drug with human serum albumin (HSA ):spectroscopic and molecular docking investigations [J].Journal of Photochemistry and Photobiology B:Biology,2012,114:132-139.
[10] 朱国飞,滕腾,邓斌.分子对接模拟与光谱法研究没食子酸丙酯和牛血清白蛋白相互作用 [J].食品工业科技,2016,37(18):158-164,191.ZHU G F,TENG T,DENG B.Study on the interaction between propyl gallate and bovine serum albumin using fluorescence spectroscopy and molecular docking [J].Science and Technology of Food Industry,2016,37(18):158-164,191.
[11] SHI M,LU Y T,WU J L,et al.Beneficial effects of theaflavins on metabolic syndrome:from molecular evidence to gut microbiome [J].International Journal of Molecular Sciences,2022,23(14):7 595.
[12] HAN S,CUI F Z,MCCLEMENTS D J,et al.Structural characterization and evaluation of interfacial properties of pea protein isolate-EGCG molecular complexes [J].Foods,2022,11(18):2 895.
[13] ABDULLAH S M S,FATMA S,RABBANI G,et al.A spectroscopic and molecular docking approach on the binding of tinzaparin sodium with human serum albumin [J].Journal of Molecular Structure,2017,1 127:283-288.
[14] LIU Y J,WU Y J,ZHANG J Y,et al.Bovine serum albumin-EGCG particles increase high internal phase Pickering emulsions stability:the leading role of EGCG concentrations[J].LWT-Food Science and Technology,2025,218:117496.
[15] 崔朋.《红茶中茶黄素类化合物的提取与鉴定 》实验综述报告[J].科技风,2022 (34):145-147.CUI P.Overview of a comprehensive experiment of extraction and identification of theaflavins from black tea [J].Technology Wind,2022 (34):145-147.
[16] VU H T,SONG F V,TIAN K,et al.Systematic characterisation of the structure and radical scavenging potency of Pu'Er tea polyphenol theaflavin [J].Organic & Biomolecular Chemistry,2019,17(46):9 942-9 950.[17] PHAN H T T,YODA T,CHAHAL B,et al.Structure-dependent interactions of polyphenols with a biomimetic membrane system [J].Biochimica et Biophysica Acta,2014,1 838(10):2 670-2 677.
[18] 张倩洁,王平礼,张冬梅,等.天然固体颗粒稳定 Pickering 乳液的研究进展及其在化妆品中的应用 [J].精细化工,2022,39(12):2 377-2 386,2 397.ZHANG Q J,WANG P L,ZHANG D M,et al.Research progress and cosmetic applications of Pickering emulsion stabilized with natural solid particles [J].Fine Chemicals,2022,39(12):2 377-2 386,2 397.
[19] 崔凤展.乳清分离蛋白纤维 Pickering 乳液体系的构建及其递送功能因子的应用 [D].咸阳:西北农林科技大学,2022:32-33.CUI F Z.Construction of whey protein isolate fibrils Pickering emulsion and its application for delivery of functional ingredients [D].Xianyang:Northwest A & F University,2022:32-33.
[20] 万俊,张孝芹,邓博文,等.糖基化结合单宁酸改性对熔球态蛋清蛋白乳化特性的影响 [J].食品与机械,2024,40(11):12-18.WAN J,ZHANG X Q,DENG B W,et al.Effects of glycosylation combined with tannic acid modification on the emulsification properties of molten globule egg white protein[J].Food & Machinery,2024,40(11):12-18.
[21] XUE F,LI C,ADHIKARI B.Physicochemical properties of soy protein isolates-cyanidin- 3-galactoside conjugates produced using free radicals induced by ultrasound [J].Ultrasonics Sonochemistry,2020,64:104990.
[22] SKRT M,BENEDIK E,PODLIPNIK Č,et al.Interactions of different polyphenols with bovine serum albumin using fluorescence quenching and molecular docking [J].Food Chemistry,2012,135(4):2 418-2 424.
[23] 孙冰玉,高远,刘琳琳,等.大豆亲脂蛋白—多酚互作及复合物性质研究进展 [J].中国食品学报,2022,22(6):382-389.SUN B Y,GAO Y,LIU L L,et al.Research progress in the interaction of soybean prolipoprotein-polyphenol and the properties of complex [J].Journal of Chinese Institute of Food Science and Technology,2022,22(6):382-389.
[24] LI Z K,WANG X H,ZHANG M,et al.The loading of epigallocatechin gallate on bovine serum albumin and pullulan-based nanoparticles as effective antioxidant [J].Foods,2022,11(24):4 074.
[25] LIU H H,WANG B,BARROW C J,et al.Relating the variation of secondary structure of gelatin at fish oil-water interface to adsorption kinetics,dynamic interfacial tension and emulsion stability [J].Food Chemistry,2014,143:484-491.
[26] XU J,HUANG Y,WEI Y,et al.Study on the interaction mechanism of theaflavin with whey protein:multi-spectroscopy analysis and molecular docking [J].Foods,2023,|12(8):1 637.
[27] LI X X,JIANG H T,PU Y J,et al.Inhibitory effect of condensed tannins from banana pulp on cholesterol esterase and mechanisms of interaction [J].Journal of Agricultural and Food Chemistry,2019,67(51):14 066-14 073.
[28] SHI J H,PAN D Q,JIANG M,et al.Binding interaction of ramipril with bovine serum albumin (BSA ):insights from multi-spectroscopy and molecular docking methods [J].Journal of Photochemistry and Photobiology B-Biology,2016,164:103-111.
[29] ZHANG R J,CHENG L R,LUO L,et al.Formation and characterisation of high-internal-phase emulsions stabilised by high-pressure homogenised quinoa protein isolate [J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2021,631:127688.
[30] CHEN Y,YAO M Y,PENG S,et al.Development of protein-polyphenol particles to stabilize high internal phase Pickering emulsions by polyphenols' structure [J].Food Chemistry,2023,428:136773.
[31] 龚穗菁.玉米醇溶蛋白纳米颗粒和纤维素纳米晶协同稳定的皮克林双重乳液 [D].无锡:江南大学,2022:2-3.GONG S Q.Pickering double emulsion synergistically stabilized by zein nanoparticles and cellulose nanocrystals [D].Wuxi:Jiangnan University,2022:2-3.
[32] WANG C Z,WU J H,WANG C H,et al.Advances in Pickering emulsions stabilized by protein particles:toward particle fabrication,interaction and arrangement [J].Food Research International,2022,157:111380.
[33] ZHAO Q L,ZAABOUL F,LIU Y F,et al.Recent advances on protein-based Pickering high internal phase emulsions (Pickering HIPEs ):fabrication,characterization,and applications [J].Comprehensive Reviews in Food Science and Food Safety,2020,19(4):1 934-1 968.
[34] KULSHRESHTHA A K,WALL O N S,WALL G M.Pharmaceutical suspensions [M].New York:Springer,2010:978.
[35] CHEN X W,FU S Y,HOU J J,et al.Zein based oil-in-glycerol emulgels enriched with β-carotene as margarine alternatives [J].Food Chemistry,2016,211:836-844.
[36] VAN VLIET T.Rheological properties of filled gels.Influence of filler matrix interaction [J].Colloid and Polymer Science,1988,266(6):518-524.
[37] 陶胜男.玉米醇溶蛋白纳米粒子和淀粉纳米晶协同稳定的Pickering 乳液 [D].无锡:江南大学,2021:61.TAO S N.Pickering emulsion symergistically stabilized by zein nanoparticles and starch nanocrystals [D].Wuxi:Jiangnan University,2021:61.
[38] BASYIGIT B,ALTUN G,YUCETEPE M,et al.Locust bean gum provides excellent mechanical and release attributes to soy protein-based natural hydrogels [J].International Journal of Biological Macromolecules,2023,231:123352.
[39] CAO M M,ZHANG X C,ZHU Y Q,et al.Enhancing the physicochemical performance of myofibrillar gels using Pickering emulsion fillers:rheology,microstructure and stability [J].Food Hydrocolloids,2022,128:107606.
[40] YUAN F,LI X D,DOU J Y,et al.Preparation of poly (butadiene-styrene-vinyl pyridine )/poly (acrylonitrile-butadiene ) core-shell nanoparticles by intermittent seeded emulsion polymerization and their catalytic latex hydrogenation [J].Catalysts,2024,14(4):277.
[41] CHENG J R,SHEN S W,YANG H G,et al.Improved physicochemical stability and bioaccessibility of astaxanthin-loaded oil-in-water emulsions by a casein-caffeic acid –glucose ternary conjugate [J].Food Research International,2023,163:112153.
[42] GAO W,WU T,CHENG Y M,et al.Highly water-resistant paper via infiltration with polymeric microspheres from nanocellulose-stabilized plant oil-derived monomer [J].International Journal of Biological Macromolecules,2024,267:131539.
[43] WANG L C,CHENG X Y,LAN H Y,et al.Development and characterization of novel ultra stable high internal phase Pickering emulsions gel:interface structure,stabilization mechanism,and applications [J].Food Hydrocolloids,2024,151:109817.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.