Abstract
［Objective］ To establish a rapid determination method for vitamin A, D3, and E content in cereal-based complementary foods for infants and young children by online solid phase extraction -two-dimensional liquid chromatography simultaneously.［Methods］ Sample saponification solution is purified by online solid -phase extraction. Then, vitamin A and E are separated and detected by the first -dimension chromatography, while vitamin D3 is preliminarily separated in the first dimension and then transferred to the second -dimension chromatography for further separation and detection, with all quantification achieved by the external standard method.［Results］］ Vitamin A,D3,and E exhibit a good linearity in the concentration ranges, with correlation coefficients (R2) being more than 0.999.The limits of quantification (LOQ ) are 1.62,0.308,and 13.9 μg/100 g for vitamins A,D3,and E,respectively.The spike recoveries range from 92.4% to 109.2%,with relative standard deviations (RSD ) between 0.36% and 3.08%. Additionally,the expanded uncertainties (k=2) for vitamin A, D3, and E are 7.04%,17.0%,and 5.30%,respectively.［Conclusion］ With high simplicity, efficiency, accuracy, and reliability, this method enhances the automation degree in sample pretreatment, meeting the needs of batch detection of fat-soluble vitamins in cereal-based |complementary foods for infants and young children.
Publication Date
6-17-2026
First Page
68
Last Page
76
DOI
10.13652/j.spjx.1003.5788.2025.80612
Recommended Citation
Qiang, LONG; Tao, ZHAO; Mei, XIAO; Haizhi, WU; Fan, ZHOU; and Zhiyong, DAI
(2026)
"Rapid determination and uncertainty evaluation of vitamins A , D 3, and E content in cereal-based complementary foods for infants and young children by online solid phase extraction-two-dimensional liquid chromatography,"
Food and Machinery: Vol. 42:
Iss.
5, Article 8.
DOI: 10.13652/j.spjx.1003.5788.2025.80612
Available at:
https://www.ifoodmm.cn/journal/vol42/iss5/8
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