Abstract
[Objective] To prepare Flammulina velutipes crude polysaccharide (FVP) and evaluate its structural characteristics and immunomodulatory activity. [Methods] FVP was prepared from Flammulina velutipes using hot-water extraction and ethanol precipitation, and its structural characteristics were characterized by combining Fourier transform infrared spectroscopy (FTIR) and high-performance size-exclusion chromatography–multi-angle laser light scattering–refractive index detection (HPSEC–MALLS–RI). The immunomodulatory activity of FVP was evaluated using RAW 264.7 macrophages and a cyclophosphamide-induced immunosuppressed mouse model. [Results] The total sugar content of FVP is (51.39 ± 1.36) g/100 g. Structural characterization results show that FVP has characteristic features of a β-type pyranose ring and exhibits a multi-component relative molecular weight distribution, with an overall weight-average relative molecular weight of 291.40. Further in vitro and in vivo bioactivity evaluations indicate that FVP has no obvious toxicity toward RAW 264.7 macrophages in the range of 3.125–600.0 μg/mL and can promote nitric oxide production and the mRNA expression levels of tumor necrosis factor-α, interleukin-1β, and interleukin-6, and significantly improve the suppression of body weight gain, the decrease of lymphocyte-related indices, and the abnormalities of immune organ indices in immunosuppressed mice. [Conclusion] The prepared FVP has favorable immunomodulatory activity and can be applied in the field of functional foods.
Publication Date
7-13-2026
First Page
124
Last Page
133
DOI
10.13652/j.spjx.1003.5788.2026.80333
Recommended Citation
Xinnuo, WANG; Chujun, PENG; Yumeng, JIN; Molan, ZHANG; and Lijun, YOU
(2026)
"Study on structural characteristics and immunomodulatory activity of Flammulina velutipes crude polysaccharide,"
Food and Machinery: Vol. 42:
Iss.
6, Article 16.
DOI: 10.13652/j.spjx.1003.5788.2026.80333
Available at:
https://www.ifoodmm.cn/journal/vol42/iss6/16
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