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Corresponding Author(s)

王恩鹏（1983—），男，长春中医药大学教授，博士。E-mail：wangep@ccucm.edu.cn

Abstract

[Objective] To comprehensively compare and screen glycoprotein preparation processes for six representative medicinal materials from the genus Panax. [Methods] Glycoprotein yield, DPPH radical scavenging capacity, hydroxyl radical scavenging capacity, and Fe2+ chelation inhibition rate were used as evaluation indicators. Glycoprotein extraction rates and antioxidant activities of six medicinal materials, including P. ginseng, P. notoginseng, P. quinquefolius, P. japonicus (T. Nees) C. A. Mey., P. stipuleanatus H. T. Tsai & K. M. Feng, and P. bipinnatifidus Seem, were compared using traditional thermal extraction and ultra-high pressure processing (UHPP). The Box–Behnken response surface method was employed to optimize UHPP parameters for P. quinquefolius glycoproteins with the highest antioxidant activity. [Results] UHPP produced higher glycoprotein extraction rates for all six Panax species compared with thermal reflux extraction. P. quinquefolius glycoproteins exhibited significantly higher DPPH radical scavenging capacity, hydroxyl radical scavenging capacity, and Fe2+ chelation inhibition rate than the other species, demonstrating the strongest antioxidant activity among UHPP-extracted glycoproteins. The optimal UHPP conditions for P. quinquefolius glycoprotein extraction were as follows: extraction time of 12 min, extraction pressure of 400 MPa, and solid-to-liquid ratio of 1:18 (g/mL). Under these conditions, the glycoprotein content reached 25.27%, deviating only 0.14% from the predicted value, representing a significant improvement over traditional reflux extraction. [Conclusion] UHPP technology can be used to prepare glycoproteins from Panax species with high antioxidant activity.

Publication Date

7-13-2026

First Page

142

Last Page

149

DOI

10.13652/j.spjx.1003.5788.2025.80516

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