Abstract
[Objective] To optimize the purification process of peanut skin polyphenols (PSPs), improve purification efficiency, and investigate their in vitro hypoglycemic activity. [Methods] Three types of PSPs (red, white, and purple) were extracted by ultrasound-assisted ethanol extraction. Seven macroporous resins were used for static adsorption and desorption of PSPs, and the resin with the best performance was selected for single-factor experiments. The components of the purified PSPs were analyzed by UPLC-ESI-QTOF-MS/MS. Using acarbose as a positive control, the inhibitory activity and inhibition types of PSPs against α-amylase and α-glucosidase were investigated. [Results] Purple peanut skins exhibited the highest contents of total phenols, total flavonoids, and anthocyanins. NKA-9 resin showed the best adsorption and desorption performance for PSPs. The optimized purification conditions were as follows: PSP concentration of 2.5 mg/mL, solution pH of 5, eluent concentration of 70%, sample loading flow rate of 2 BV/h, and elution flow rate of 1 BV/h. Under these conditions, the purity of PSPs reached 77.42%, representing a 2.27-fold increase. A total of 19 phenolic compounds, including procyanidins B1, B2, B4, gallic acid, and kaempferol, were identified in the purified PSPs. The half-inhibitory concentrations (IC50) of PSPs against α-amylase and α-glucosidase were 96.33 and 7.01 μg/mL, respectively, and both exhibited mixed-type inhibition. [Conclusion] Purified PSPs exhibit excellent in vitro hypoglycemic activity and have potential for development and utilization as functional ingredients in hypoglycemic foods.
Publication Date
9-20-2026
First Page
159
Last Page
168
DOI
10.13652/j.spjx.1003.5788.2025.80837
Recommended Citation
Wenda, Zhou; Yunhui, Yin; and Jingxuan, Fu
(2026)
"Purification , characterization , and hypoglycemic activity of peanut coat polyphenols,"
Food and Machinery: Vol. 42:
Iss.
8, Article 20.
DOI: 10.13652/j.spjx.1003.5788.2025.80837
Available at:
https://www.ifoodmm.cn/journal/vol42/iss8/20
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