•  
  •  
 

Corresponding Author(s)

郭庆彬(1983—),男,天津科技大学教授,博士生导师,博士。E-mail:[email protected]

Abstract

[Objective] To expand the application fields of distillers dried grains with solubles and improve its market value. [Methods] Arabinoxylan (AX) was extracted from distillers dried grains with solubles and modified to prepare AX with different molecular structures. AX was then reacted with soy protein isolate (SPI) via the Maillard reaction to prepare the AX-SPI covalent complex. The structure of the complex was characterized using circular dichroism spectroscopy, Fourier transform infrared spectroscopy, particle size, and zeta potential, and its emulsifying properties were studied. [Results] The AX-SPI covalent complex is formed by SPI and AX via the Maillard reaction, and differences in the polysaccharide molecular structure affect the extent of the Maillard reaction. As the relative molecular mass and chain length of the polysaccharide increase, the difficulty of its attachment to the protein peptide chain also increases. Compared with SPI, the protein solubility of the covalent complex increases by 16.29%, indicating that the solubility of the complex in the isoelectric point region is significantly improved. The average particle size (0.799 μm) of the emulsion stabilized by AX-SPI is small and evenly distributed, and the absolute value of the zeta potential is high (38.19 mV). [Conclusion] The AX-SPI covalent complex exhibits better emulsification and stability than SPI. Moreover, the emulsifying properties of the complex increase with the decrease in the relative molecular mass of the polysaccharide. When the relative molecular mass is similar, a shorter polysaccharide chain length results in better emulsifying properties.

Publication Date

9-20-2026

First Page

26

Last Page

32

DOI

10.13652/j.spjx.1003.5788.2024.80618

References

[1] Ray G W,Li X H,He S Q,et al.A review on the use of distillers dried grains with solubles (DDGS) in aquaculture feeds [J].Annals of Animal Science,2022,22(1):21-42.

[2] Mlynekova Z,Ceresnakova Z,Formelova Z,et al.Chemical and nutritional characteristics of different types of DDGS for ruminants [J].Journal of Animal and Feed Sciences,2012,21(3):425-435.

[3] Chen H,Liu Y,Yang T,et al.Interactive effects of molecular weight and degree of substitution on biological activities of arabinoxylan and its hydrolysates from triticale bran [J].International Journal of Biological Macromolecules,2021,166:1 409-1 418.

[4] Hattori M,Okada Y,Takahashi K.Functional changes in β-lactoglobulin upon conjugation with carboxymethyl cyclodextrin[J].Journal of Agricultural and Food Chemistry,2000,48(9):3 789-3 794.

[5] Sakamoto H,Kumazawa Y,Motoki M.Strength of protein gels prepared with microbial transglutaminase as related to reaction conditions [J].Journal of Food Science,1994,59(4):866-871.

[6] Dursun Capar T,Yalcin H.Protein/polysaccharide conjugation via Maillard reactions in an aqueous media:impact of protein type,reaction time and temperature [J].LWT-Food Science & Technology,2021,152:112252.

[7] Pirestani S,Nasirpour A,Keramat J,et al.Structural properties of canola protein isolate-gum Arabic Maillard conjugate in an aqueous model system [J].Food Hydrocolloids,2018,79:228-234.

[8] Liu J H,Ru Q M,Ding Y T.Glycation a promising method for food protein modification:physicochemical properties and structure,a review [J].Food Research International,2012,49(1):170-183.

[9] 陈澄,赵璟仪,杨姣,等.不同相对分子质量的糖对乳清分离蛋白糖基化反应产物功能特性的影响 [J].食品科技,2021,46(11):229-235.Chen C,Zhao J Y,Yang J,et al.Effect of different relative molecular mass sugars on the functional properties of whey protein isolate glycosylation reaction products [J].Food Science and Technology,2021,46(11):229-235.

[10] Kang J,Guo Q B,Shi Y C.Molecular and conformational properties of hemicellulose fiber gum from dried distillers grains with solubles [J].Food Hydrocolloids,2018,80:53-59.

[11] 朱雪芮.乳清分离蛋白—阿拉伯木聚糖复合物的制备与功能性质研究 [D].天津:天津科技大学,2021:2.Zhu X R.Preparation and functional properties of whey protein isolate-arabinoxylan conjugates [D].Tianjin:Tianjin University of Science and Technology,2021:2.

[12] Lowry O,Rosebrough N,Farr A L,et al.Protein measurement with the folin phenol reagent [J].Journal of Biological Chemistry,1951,193(1):265-275.

[13] Dubois M,Gilles K A,Hamilton J K,et al.Colorimetric method for determination of sugars and related substances [J].Analytical Chemistry,2002,28(3):350-356.

[14] Blumenkrantz N,Asboe-Hansen G.New method for quantitative determination of uronic acids [J].Analytical Biochemistry,1973,54(2):484-489.

[15] Liu H H,Fan Y L,Wang W H,et al.Polysaccharides from lycium barbarum leaves:isolation,characterization and splenocyte proliferation activity [J].International Journal of Biological Macromolecules,2012,51(4):417-422.

[16] Guo Q B,Cui S W,Wang Q,et al.Structural characterization of a low-molecular-weight heteropolysaccharide (glucomannan) isolated from Artemisia sphaerocephala Krasch[J].Carbohydrate Research,2012,350:31-39.

[17] Zhu X R,Meng L Y,Song S,et al.Insight into the mechanisms of the excellent emulsification properties of whey protein isolate-arabinoxylan conjugates [J].Bioactive Carbohydrates and Dietary Fibre,2022,27:100312.

[18] Allahdad Z,Varidi M,Zadmard R,et al.Binding of β-carotene to whey proteins:multi-spectroscopic techniques and docking studies [J].Food Chemistry,2019,277:96-106.

[19] Liu L,Li X D,Du L L,et al.Effect of ultrasound assisted heating on structure and antioxidant activity of whey protein peptide grafted with galactose [J].LWT-Food Science and Technology,2019,109:130-136.

[20] Liu Y,Zhao G L,Zhao M M,et al.Improvement of functional properties of peanut protein isolate by conjugation with dextran through Maillard reaction [J].Food Chemistry,2012,131(3):901-906.

[21] Xu Y,Yang N,Yang J X,et al.Protein/polysaccharide intramolecular electrostatic complex as superior food-grade foaming agent [J].Food Hydrocolloids,2020,101:105474.

[22] Hattori M,Nagasawa K,Ametani A,et al.Functional changes in β-lactoglobulin by conjugation with carboxymethyl dextran[J].Journal of Agricultural and Food Chemistry,1994,42(10):2 120-2 125.

[23] Mangavel C,Barbot J,Popineau Y,et al.Evolution of wheat gliadins conformation during film formation:a fourier transform infrared study [J].Journal of Agricultural and Food Chemistry,2001,49(2):867-872.

[24] Chen L,Chen J S,Ren J Y,et al.Effects of ultrasound pretreatment on the enzymatic hydrolysis of soy protein isolates and on the emulsifying properties of hydrolysates [J].Journal of Agricultural and Food Chemistry,2011,59(6):2 600-2 609.

[25] Mu L X,Zhao M M,Yang B,et al.Effect of ultrasonic treatment on the graft reaction between soy protein isolate and gum acacia and on the physicochemical properties of conjugates [J].Journal of Agricultural and Food Chemistry,2010,58(7):4 494-4 499.

[26] Martins S I F S,Jongen W M F,Van Boekel M A J S.A review of Maillard reaction in food and implications to kinetic modelling [J].Trends in Food Science & Technology,2000,11(9/10):364-373.

[27] 张朵朵,朱婷伟,陈复生,等.小麦麸皮阿拉伯木聚糖/豌豆分离蛋白复合物的乳化性能研究 [J].食品研究与开发,2022,43(4):1-8.Zhang D D,Zhu T W,Chen F S,et al.Emulsifying properties of wheat bran arabinoxylan/pea protein isolate complex [J].Food Research and Development,2022,43(4):1-8.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.