Screening and identification of a Vibrio parahaemolyticus -antagonistic fusion Weissella confusa Y10-1 and evaluation of its bacteriostatic activity

Authors

• Yuan Baolong, School of Marine Sciences and Biotechnology , Guangxi Minzu University , Nanning , Guangxi 530006 , China;Guangxi Key Laboratory of Polysaccharide Materials and Modification , Nanning , Guangxi 530006 , China
• Yi Han, School of Marine Sciences and Biotechnology , Guangxi Minzu University , Nanning , Guangxi 530006 , China;Guangxi Key Laboratory of Polysaccharide Materials and Modification , Nanning , Guangxi 530006 , China
• Shen Naikun, School of Marine Sciences and Biotechnology , Guangxi Minzu University , Nanning , Guangxi 530006 , China;Guangxi Key Laboratory of Polysaccharide Materials and Modification , Nanning , Guangxi 530006 , ChinaFollow
• Liang Ying, School of Marine Sciences and Biotechnology , Guangxi Minzu University , Nanning , Guangxi 530006 , China;Guangxi Key Laboratory of Polysaccharide Materials and Modification , Nanning , Guangxi 530006 , China
• Tang Jiahao, School of Marine Sciences and Biotechnology , Guangxi Minzu University , Nanning , Guangxi 530006 , China;Guangxi Key Laboratory of Polysaccharide Materials and Modification , Nanning , Guangxi 530006 , China
• Zhang Hongyan, School of Marine Sciences and Biotechnology , Guangxi Minzu University , Nanning , Guangxi 530006 , China;Guangxi Key Laboratory of Polysaccharide Materials and Modification , Nanning , Guangxi 530006 , China
• Zhang Bin, Guangxi Academy of Fisheries Sciences , Nanning , Guangxi 530021 , China;Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture , Nanning , Guangxi 530021 , China

Corresponding Author(s)

申乃坤（1980—），男，广西民族大学教授，博士。E-mail：[email protected]

Abstract

[Objective] To isolate and screen lactic acid bacteria from the intestinal tract of marine fish with inhibitory activity against Vibrio parahaemolyticus and to investigate their antibacterial activity. [Methods] Using V.parahaemolyticus as the indicator bacterium, preliminary antibacterial screening was performed. The strain was identified through morphological, physiological, and biochemical characteristics as well as 16S rRNA gene sequence analysis. The antibacterial spectrum of the strain was determined, and the antibacterial stability, antibacterial mechanisms, and minimum inhibitory concentration (MIC ) of its cell -free supernatant (CFS ) were analyzed. [Results] A strain, Y10-1, showing strong antagonistic activity against V.parahaemolyticus was screened and identified as Weissella |confusa. The antibacterial substances produced by this strain demonstrated broad -spectrum antibacterial activity, were heat resistant (100 ℃ for 15 min ), and exhibits inhibitory activity against V.parahaemolyticus within a pH range of 2.0~9.0. The MIC against V.parahaemolyticus was 5%. Under the condition of 3×MIC, the antagonistic effect against V.parahaemolyticus was the most pronounced. [Conclusion] The strain Y 10-1 screened in this study exhibits good antagonistic activity against V.parahaemolyticus and has considerable potential for development and application in food preservation.

Publication Date

10-21-2026

First Page

10

Last Page

18

DOI

10.13652/j.spjx.1003.5788.2025.80863

Recommended Citation

Baolong, Yuan; Han, Yi; Naikun, Shen; Ying, Liang; Jiahao, Tang; Hongyan, Zhang; and Bin, Zhang (2026) "Screening and identification of a Vibrio parahaemolyticus -antagonistic fusion Weissella confusa Y10-1 and evaluation of its bacteriostatic activity," Food and Machinery: Vol. 42: Iss. 9, Article 2.
DOI: 10.13652/j.spjx.1003.5788.2025.80863
Available at: https://www.ifoodmm.cn/journal/vol42/iss9/2

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