Abstract
[Objective] To evaluate the measurement uncertainty in the determination of total plate count and coliform bacteria in cooked foods, thereby improving the accuracy of test results. [Methods] The plate counting methods specified in GB 4789.2—2022 and GB 4789.3—2025 were employed to detect 18 cooked food samples under two conditions (A and B ). The combined standard uncertainty (uc(y)) and expanded uncertainty (U=2×uc(y)) were calculated in accordance with RB/T 228—2023. [Results] Based on intralaboratory reproducibility, the uc(y) for total plate count determination was 0.030 3 (U=0.060 6), and that for coliform bacteria determination was 0.179 7 (U=0.359 7). When all uncertainty components were considered, the uc(y) for total plate count determination of Sample 1 under Condition A increased to 0.109 4 (U=0.218 8), and that for coliform bacteria determination increased to 0.242 4 (U=0.484 8). [Conclusion] The primary factors affecting the measurement uncertainty of this method include technical uncertainty, matrix uncertainty, and distribution uncertainty. The application of RB/T 228—2023 for uncertainty assessment can improve the accuracy of test results.
Publication Date
10-21-2026
First Page
48
Last Page
52
DOI
10.13652/j.spjx.1003.5788.2025.80729
Recommended Citation
Weiping, Liu; Jianchun, Wan; Ying, Han; Pengfei, Jiang; and Shixiang, Li
(2026)
"Measurement uncertainty assessment for total aerobic bacterial count and coliforms in cooked foods according to RB/T 228,"
Food and Machinery: Vol. 42:
Iss.
9, Article 6.
DOI: 10.13652/j.spjx.1003.5788.2025.80729
Available at:
https://www.ifoodmm.cn/journal/vol42/iss9/6
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