Abstract
In this study, the experimental condition for qualitative analysis of saccharides in beer was optimized by choosing different matrices, including. 2,5-dihydroxybenzoic acid (2,5-DHB), super DHB, 2,4,6-trihydroxyacetophenone, and setting different beer concentrations from undiluted to 1 000-fold dilution. Results indicated that 2,4,6-trihydroxyacetophenone was better than 2,5-dihydroxybenzoic acid and super DHB in the application of characterizing saccharides in beer by MALDI TOF MS. When the beer sample is diluted 10 times with 2,4,6-trihydroxyacetophenone as matrix, MALDI TOF MS performed well in qualitative analysis of saccharides in beer.
Publication Date
4-28-2020
First Page
86
Last Page
90
DOI
10.13652/j.issn.1003-5788.2020.04.016
Recommended Citation
Xian-ren, MENG; Ming-yong, XIE; Shao-ping, NIE; and jun-qiao, WANG
(2020)
"Optimization on qualitative analysis of saccharides in beer by MALDI-TOF MS,"
Food and Machinery: Vol. 36:
Iss.
4, Article 16.
DOI: 10.13652/j.issn.1003-5788.2020.04.016
Available at:
https://www.ifoodmm.cn/journal/vol36/iss4/16
References
[1] ARAJO A S,DA ROCHA L L,TOMAZELA D M,et al.Electrospray ionization mass spectrometry fingerprinting of beer[J].Analyst,2005,130(6):884-889.
[2] MAURI P,MINOGGIO M,SIMONETTI P,et al.Analysis of saccharides in beer samples by flow injection with electrospray mass spectrometry[J].Rapid Communications in Mass Spectrometry,2002,16(8):743-748.
[3] DELL A,MORRIS H R.Glycoprotein structure determination by mass spectrometry[J].Science,2001,291(5 512):2 351-2 356.
[4] SAMYN B,DEBYSER G,SERGEANT K,et al.A case study of de novo sequence analysis of N-sulfonated peptides by MALDI TOF/TOF mass spectrometry[J].Journal of the American Society for Mass Spectrometry,2004,15(12):1 838-1 852.
[5] GIRIBALDI M,PERUGINI I,SAUVAGE F X,et al.Analysis of protein changes during grape berry ripening by 2-DE and MALDI-TOF[J].Proteomics,2007,7(17):3 154-3 170.
[6] MURPHY R C,HANKIN J A,BARKLEY R M.Imaging of lipid species by MALDI mass spectrometry[J].Journal of Lipid Research,2009,50(Suppl):S317-S322.
[7] VENKATARAMAN G,SHRIVER Z,RAMAN R,et al.Sequencing complex polysaccharides[J].Science,1999,286(5 439):537-542.
[8] YANG Ying,CHIEN Ming-jien.Characterization of grape procyanidins using high-performance liquid chromatography/mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry[J].Journal of Agricultural and Food Chemistry,2000,48(9):3 990-3 996.
[9] BONATTO C C,SILVA L P.Cocoa content influences chocolate molecular profile investigated by MALDI-TOF mass spectrometry[J].Journal of the Science of Food and Agriculture,2015,95(8):1 753-1 756.
[10] QU Liang-liang,JIANG Yu-ming,HUANG Xue-yong,et al.High-throughput monitoring of multiclass syrup adulterants in honey based on the oligosaccharide and polysaccharide profiles by MALDI mass spectrometry[J].Journal of Agricultural and Food Chemistry,2019,67(40):11 256-11 261.
[11] PARK E,YANG H,KIM Y,et al.Analysis of oligosaccharides in beer using MALDI-TOF-MS[J].Food Chemistry,2012,134(3):1 658-1 664.
[12] KARAS M,EHRING H,NORDHOFF E,et al.Matrix-assisted laser desorption/ionization mass spectrometry with additives to 2,5-dihydroxybenzoic acid[J].Organic Mass Spectrometry,1993,28(12):1 476-1 481.
[13] HSU Nien-yeen,YANG Wen-bin,WONG Chi-huey,et al.Matrix-assisted laser desorption/ionization mass spectrometry of polysaccharides with 2′,4′,6′-trihydroxyacetophenone as matrix[J].Rapid Communications in Mass Spectrometry:An International Journal Devoted to the Rapid Dissemination of Up-to-the-Minute Research in Mass Spectrometry,2007,21(13):2 137-2 146.
[14] THOMAS B R,BRANDLEY B K,RODRIGUEZ R L.Rapid analysis of saccharides in beer via fluorescence-assisted carbohydrate electrophoresis[J].Journal of the American Society of Brewing Chemists,2000,58(3):124-127.
[15] 潘媛媛,梁立娜,蔡亚岐,等.高效阴离子交换色谱—脉冲安培检测法分析啤酒和麦汁中的糖[J].色谱,2008(5):626-630.
[16] 申德超,李宏军,孟阳.挤压膨化大米辅料麦汁和啤酒的糖类组分分析[J].农业机械学报,2004,35(6):134-137.