Determination of icariin and other 20 functional components in food by liquid chromatography tandem mass spectrometry

Authors

FAN Xiao-long, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430000, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430000, China
CHEN Ran, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430000, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430000, China
WU Wan-qin, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430000, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430000, China
LIU Guo-jiao, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430000, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430000, China
HUANG Kun, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430000, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430000, China
JIANG Feng, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430000, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430000, China

Abstract

A method for the quantitative determination of icariin, hypericin and Psoralen in mix liquor, beverage sample and tea substitutes were established by ultra-high-performance liquid chromatography tandem mass spectrometry. Samples were extracted by methanol water solution, diluted, purified, extracted, centrifuged, filtered, and determined by Thermo Acclain RSLC C₁₈ (2.6 μm, 2.1 mm×100 mm) chromatographic column. The determination was carried out under the mode of electrospray positive and negative ion multi-reaction monitoring scanning (MRM) with acetonitrile and water. The maximum linear detection concentration of 20 compounds was 500 ng/mL, the correlation coefficient (R²) was greater than 0.99, and the quantitative limit was 0.5～5.0 mg/kg. The recovery test of standard addition was carried out at three levels of 1, 2 and 10 times limit of quantitation. The average recovery rate of 20 compounds was between 72.80%～107.75%, and the relative deviation was less than 10%. Conclusion: The method is easy to operate, simple, fast, clean, high sensitivity and good repeatability.

Publication Date

1-28-2021

First Page

86

Last Page

93

DOI

10.13652/j.issn.1003-5788.2021.01.013

Recommended Citation

Xiao-long, FAN; Ran, CHEN; Wan-qin, WU; Guo-jiao, LIU; Kun, HUANG; and Feng, JIANG (2021) "Determination of icariin and other 20 functional components in food by liquid chromatography tandem mass spectrometry," Food and Machinery: Vol. 37: Iss. 1, Article 13.
DOI: 10.13652/j.issn.1003-5788.2021.01.013
Available at: https://www.ifoodmm.cn/journal/vol37/iss1/13

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