•  
  •  
 

Abstract

Extracted conditions for citrinin were optimized in sufu. Citrinin was extracted completely by toluene-ethyl acetate-formic acid(7∶3∶1, V/V) at 50 ℃, with 10 min ultrasonic processing. A method for determining citrinin in sufu was established by high performance liquid chromatography (HPLC) with fluorescence detection. Citrinin was separated by mobile phase of water(pH 2.5)- acetonitrile (70∶30) on Hypersil ODS 2 column. The results were showed that there was good linear relationship between the fluorescence detection values and citrinin concentration in the range of 0.01~100.00 μg/mL (R2=0.999 99). The limit of detection was 0.005 mg/kg and the recovery of citrinin were between 90.4% and 107.9% and relative standard deviation(RSD, n=6) value were less than 6%.

Publication Date

4-28-2015

First Page

102

Last Page

105

DOI

10.13652/j.issn.1003-5788.2015.02.021

References

[1] 张国艳, 石彦国. 腐乳生产回顾[J]. 食品与发酵工业, 1993(6): 72~74.
[2] Wong H C, Koehler P E. production and isolation of an antibiotic from monascus purpureus and its relationship to pigment production [J]. Journal of Food Science,1981, 46(2):589~592.
[3] Blanc P J, Laussac J P, Bars J, et al. Characterization of monascidin A from Monascus as citrinin[J]. International Journal of Food Microbiology, 1995(27): 201~213.
[4] Chiba H, Yoshikawa M. Opioid antagonist peptides derived from k-casein[J]. Jourmal of Dairy Research, 1989, 3(56): 363~366.
[5] Sabater-Vilar M, Maas R F M, Fink-Gremmels J. Mutagenicity of commercial Monascus fermentation products and the role of citrinin contamination[J]. Mutation Research, 1999(444): 7~16.
[6] 许赣荣, 卢晨, 穆晓青, 等. 部分红曲霉菌株产桔霉素的研究[J]. 无锡轻工大学学报, 2000, 19(1):58.
[7] 李凤琴, 许赣荣, 李玉伟, 等. 食品工业用红曲菌株产桔霉素能力的研究[J]. 卫生研究, 2003(6):16~20.
[8] 李凤琴, 许赣荣, 李玉伟, 等. 国产红曲制品中桔霉素污染水平的研究[J]. 卫生研究, 2005(4):451~454.
[9] Li Xue-Mei, Shen Xing-Hai, Xue Lan, et al. A validated RP—HPLC method for the determination of citrinin in Xuezhikang capsule and other monascus-fermented products[J]. E-Journal of Chemistey, 2012, 9(1):260~266.
[10] 李燕, 仇峰, 杨美华, 等. 高效液相色谱-串联质谱法测定中药中桔青霉素[J]. 药物分析杂志, 2011, 31(9):1 726~1 730.
[11] 文镜, 徐东. 红曲中桔霉素的HPLC苯基柱分离分析[J]. 中国酿造, 2010(6):148~151.
[12] 郭雪梅, 许杨, 刘仁荣. 免疫亲和柱-高效液相色谱法检测红曲米中的桔霉素[J]. 食品与发酵工业, 2007, 33(10): 148~151.
[13] 柴秋儿, 田亚琼, 钟其顶, 等. 食品添加剂红曲红中桔霉素检测方法的研究[J]. 食品与发酵工业, 2008, 34(11): 144~148.
[14] 孙伟伟, 王静, 吴彩梅. 腐乳中存在的安全隐患[J]. 食品研究与开发, 2009, 30(4): 156~160.
[15] 国家认证认可监督管理委员会. SN/T 2916—2011 出口食品中桔霉素的测定方法 免疫亲和柱净化—高效液相色谱法[S]. 北京: 中国标准出版社, 2011.
[16] 国家认证认可监督管理委员会. SN/T 2426—2010 进出口粮谷中桔霉素含量检测方法 液相色谱法[S]. 北京:中国标准出版社, 2010.
[17] 文镜, 贾继春. 高效液相色谱法测定红曲醋中桔霉素含量[J]. 中国酿造, 2008(9): 76~79.
[18] 黄志兵, 李燕萍, 王延华, 等. HPLC法测定橙色红曲菌As3.4384及其诱变体发酵产物中的桔霉素[J]. 食品科学, 2006, 27(12): 614~617.
[19] 陈蕴, 许赣荣, 虞慧玲. 红曲桔霉素高效液相色谱测定条件的优化[J]. 食品与发酵工业, 2004, 30(1): 118~123.
[20] 王彩霞, 肖杰, 孙爱东, 等. 高效液相色谱法检测红曲米中的桔霉素[J]. 中国粮油学报, 2010, 25(7): 125~128.
[21] 靳赛, 尤晓颜, 王玉芬, 等. 红曲米中桔霉素的高效液相色谱法检测[J]. 河南科学, 2005, 23(2): 204~206.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.