Corn protein hydrolysates of high concentration were prepared by optimizing the hydrolyzing conditions of alcalase and flavourzyme respectively. Both of which were applied synergistically to hydrolyze corn gluten to obtain antioxidative corn protein hydrolysates. The results showed that the optimal hydrolysis by Alcalase was obtained at 50 ℃, pH 7.7 and E/S of 2% (V/m) for reaction time of 75 minutes, and under the optimized conditions, DPPH radical scavenging activity and reducing power of the hydrolysate were 74.34% and 0.984 respectively; the optimal hydrolysis by Flavourzyme was reacted at 53 ℃, pH 6.4 and E/S of 5% (m/m) for reaction time of 50 minutes, and under the optimized condition, DPPH radical scavenging activity and reducing power of the hydrolysate were 70.55% and 0.715 respectively. During the process of dual-enzyme hydrolyzing corn gluten, antioxidant activities of corn protein hydrolysate that catalyzed by Alcalase+Flavourzyme were higher than that by Flavourzyme+Alcalase. At 110 min, DPPH radical scavenging value and reducing power of corn protein hydrolysate that catalyzed by Alcalase+Flavourzyme reached the highest in the whole reaction process, which were 91.32% and 1.341 respectively.

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[1] 杨双, 郑喜群, 刘晓兰, 等. 水解时间对玉米蛋白粉酶解物抗氧化活性的影响[J]. 食品与机械, 2014, 30(3): 154~158.
[2] 王层飞, 李忠海, 袭吉军, 等. 生物活性肽的保健功能及其在食品工业中的应用研究[J]. 食品与机械,2008, 24(3): 128~131.
[3] 王俊彤, 郑喜群, 刘晓兰. 酶解玉米黄粉制备降血压肽的研究[J]. 食品与机械, 2013, 29(3): 212~215.
[4] 郭庆启, 张乃珣, 王群, 等. 玉米蛋白酶解工艺的优化及酶解液对乙醇脱氢酶活性的影响[J]. 食品科学, 2013, 34(13): 170~174.
[5] Zhang Gui-xiang, Sun Na-xin, Zheng Ming-yang, et al. Preparation of high F-value oligopeptides from corn gluten meal and its anti-fatigue functions[J]. Biomedical Engineering and Biotechnology, 2012(1): 429~432.
[6] 郑喜群. 玉米黄粉的酶解工艺与抗氧化活性肽的制备[D]. 北京: 中国农业大学, 2006.
[7] 陈星, 李晓磊, 吴琼. 不同蛋白酶酶解产物活性大豆肽分子量分布状态的研究[J]. 西南大学学报(自然科学版), 2010, 32(7): 1~7.
[8] Lee J, Koo N, Min D B.Reactive oxygen species aging and antioxidative nutraceuticals[J]. Comprehensive Reviews in Food Science and Food Safety, 2004, 3(1): 21~33.
[9] 吴建中. 大豆蛋白的酶法水解及产物抗氧化活性的研究[D]. 广州: 华南理工大学, 2003.
[10] Hide Yukil, Kaharat. Superoxide and 1,1-dipheny1-2-pieryhydrazyl radical-scavenging activities of soyasaponin related to gallic acid[J]. Biosci. Biotechnol Biochem., 2001, 65(10): 2 162~2 165.
[11] Zhou Ke-quan, Sun Shi, Canning C. Production and functional characterization of antioxidative hydrolysates from corn protein via enzymatic hydrolysis and ultrafiltration[J]. Food Chemistry, 2012, 135(3): 1 192~1 197.
[12] Zheng Xi-qun, Li Li-te, Liu Xiao-lan, et al. Production of hydrolysate with antioxidative activity by enzymatic hydrolysis of extruded corn gluten[J]. Appl Micro Biol Biotechnol, 2006(73): 763~770.
[13] 宋占兰, 郑喜群, 刘晓兰. Alcalase酶解高底物浓度玉米蛋白工艺优化[J]. 食品与机械, 2011, 27(6): 226~231.
[14] 陈毓荃, 马静芳, 文建雷, 等. 生物化学实验方法和技术[M]. 北京: 科学出版社, 2002.
[15] Mina Memarpoor-Yazdi, Hanie Mahaki, Hadi Zare-Zardini. Antioxidant activity of protein hydrolysates and purified peptides from Zizyphus jujube fruits[J]. Journal of Functional Foods, 2013, 5(1): 62~70.
[16] 荣建华, 李小定, 谢笔钧. 大豆肽体外抗氧化效果的研究[J]. 食品科学, 2002, 23(11): 118~120.
[17] Zhang Yu-feng, Duan Xiu, Zhuang Yong-liang. Purification and characterization of novel antioxidant peptides from enzymatic hydrolysates of tilapia (Oreochromis niloticus) skin gelatin[J]. Peptides, 2012(38): 13~21.
[18] Li Xiu-xia, Han Lu-jia, Chen Long-jian. In vitro antioxidant activity of protein hydrolysates prepared from corn gluten meal[J]. Journal of the Science of Food and Agriculture, 2008, 88(9): 1 660~1 666.
[19] 陈星, 李晓磊, 吴琼. 不同蛋白酶酶解产物活性大豆肽分子量分布状态的研究[J]. 西南大学学报(自然科学版), 2010, 32(7): 1~7.
[20] 王晓杰, 郑喜群, 胡立玉. 双酶分布水解时间对玉米肽功能性质的影响[J]. 食品工业科技, 2013, 34(8): 223~227.

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