The hydrolysis processes of rice protein by had been studied 9 proteinases including Neutrase, Acalase 2.4 L, Papain, Pepsin, Trypsin, Flavourzyme, Acid protease, Protamex and Bromelain, and the immune activities of 9 protein hydrolysate had been considered. The results showed that Trypsin can be used as the optimum hydrolytic enzymes for the preparation of rice immune active peptide whose value-added index (stimulating index, SI) to mice macrophage (RAW264.7) is 1.597 (1 000 μg/mL); the enzymatic hydrolysis had the highest percentage of components with relative molecular mass <1 000, to 74.11%.

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[1] 刘笑然, 兰敦臣, 李越. 2014 年中国稻米产业研究[J]. 中国粮食经济, 2014 ,8(12):42~47.
[2] 冯慧阳. 基于第六次人普数据谈中国人口老龄化新变化[J]. 商业文化 (上半月), 2011(8): 112.
[3] Duarte J, Vinderola G, Ritz B, et al. Immunomodulating capacity of commercial fish protein hydrolysate for diet supplementation[J]. Immunobiology, 2006, 211(5): 341~350.
[4] Hou Hu, Fan Yan, Li Ba-fang, et al. Purification and identification of immunomodulating peptides from enzymatic hydrolysates of Alaska pollock frame[J]. Food Chemistry, 2012, 134(2): 821~828.
[5] Chen Jun-rong, Suetsuna K, Yamauchi F. Isolation and characterization of immunostimulative peptides from soybean[J]. The Journal of Nutritional Biochemistry, 1995, 6(6): 310~313.
[6] Kong Xiang-zhen, Guo Ming-ming, Hua Yu-fei, et al. Enzymatic preparation of immunomodulating hydrolysates from soy proteins[J]. Bioresource Technology, 2008, 99(18): 8 873~8 879.
[7] Kim E K, Kim Y S, Hwang J W, et al. Purification of a novel nitric oxide inhibitory peptide derived from enzymatic hydrolysates of Mytilus coruscus[J]. Fish & Shellfish Immunology, 2013, 34(6): 1 416~1 420.
[8] 黄文凯.膜分离方法制备免疫活性大豆肽的研究[D].无锡:江南大学, 2007.
[9] 大连轻工业学院.食品分析[M].北京:中国轻工业出版社,1998.
[10] Ting Feng-wu, Chiung Yuehhsu, Huei Sheng-huang, et al. Proteomic analysis of pycnogenol effects in RAW 264.7 macrophage reveals induction of cathepsin D expression and enhancement of phagocytosis[J]. Journal of Agricultural and Food Chemistry, 2007,55(24): 9 784~9 791.
[11] 赵亚华,高向阳.生物化学实验技术教程[M].广州:华南理工大学出版社,2000.
[12] 李慧静.超高静压协同酶法降低专用大豆分离蛋白致敏性的研究[D].无锡:江南大学, 2013.
[13] 方海红,方海红,胡好远,等.微生物碱性蛋白酶的研究进展[J].微生物学通报,2002, 29(2):57~59.
[14] 王彦荣,孟祥春,任连彬,等.酸性蛋白酶生产与应用的研究[J].酿酒, 2003, 30(3):16~18.
[15] 张亚飞.小麦蛋白Alcalase水解物中免疫活性肽的研究[D].无锡:江南大学,2006.
[16] Kong Xiang-zhen, Guo Ming-ming, Hua Yu-fei, et al. Enzymatic preparation of immunomodulating hydrolysates from soy proteins[J]. Bioresource Technology, 2008, 99(18): 8 873~8 879.
[17] Takahashi M, Moriguchi S, Yoshikawa M, et al. Isolation and characterization of oryzatensin: a novel bioactive peptide with ileum-contracting and immunomodulating activities derived from rice albumin[J]. Biochemistry and Molecular Biology International, 1994,33(6):1 151~1 158.
[18] Hata I, Higashiyama S, Otani H. Identification of a phosphopeptide in bovine αs1-casein digest as a factor influencing proliferation and immunoglobulin production in lymphocyte cultures[J]. Journal of Dairy Research, 1998, 65(4): 569~578.
[19] Meisel H. Biochemical properties of bioactive peptides derived from milk proteins: potential nutraceuticals for food and pharmaceutical applications[J]. Livestock Production Science, 1997, 50(1): 125~138.
[20] Adibi SA. Intestinal transport of dipeptides in man: relative importance of hydrolysis and intact absorption[J]. Journal of Clinical Investigation, 1971, 50(11):2 266~2 275.
[21] 夏树华.螺蛳中生物活性物质的研究[D].无锡:江南大学,2006.
[22] Leiros H K S, Brandsdal B O, Andersen O A, et al. Trypsin specificity as elucidated by LIE calculations, X‐ray structures, and association constant measurements[J]. Protein Science, 2004, 13(4): 1 056~1 070.
[23] 胡娟.酶解鱼鳞明胶制备生物活性肽的研究[D].广州:华南理工大学,2010.



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