Abstract
The recombinant protein was induced by transforming recombinant plasmid pET-32a (+)-Ara h 2.02 into Rosetta (DE3)，and identified by SDS—PAGE and Western Blotting. The results showed that the molecule weight of His-Ara h 2.02 fusion protein was about 38 kDa. At the same time, effect of IPTG concentration, shaking speed, induced temperature and time on the production of soluble recombinant protein were determined. The best conditions were as followed, the IPTG concentration was 0.3 mmol/L, the shaking speed was 220 r/min, the induction temperature was 37 ℃and the induction time was two hours, respectively.
Publication Date
4-28-2015
First Page
43
Last Page
46,156
DOI
10.13652/j.issn.1003-5788.2015.02.009
Recommended Citation
Qin, CAI; Wenju, ZHANG; and Qin, CHEN
(2015)
"Studies on prokaryotic expression conditions of peanut allergen Ara h 2.02,"
Food and Machinery: Vol. 31:
Iss.
2, Article 9.
DOI: 10.13652/j.issn.1003-5788.2015.02.009
Available at:
https://www.ifoodmm.cn/journal/vol31/iss2/9
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