A method was developed for simultaneous determination of 8 β-lactams in goat milk by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLC—MS/MS). The milk was extracted with acetonitrile, purified with C18 and PSA sorbent and separated by an ACQUITY UPLC BEH C18 column using water (containing 0.1% formic acid) and acetonitrile as mobile phase. The mass spectrometric acquisitions were carried out by means of multiple reaction monitoring (MRM) in positive ionization mode. The linear relation of eight β-lactams was good and the correlation coefficient was more than 0.986. The recovery rates of the target analyte were between 83.8%～95.4%. The relative standard deviations were less than 6.5%. The limit of detection (LOD) of 8 β-lactams was 0.25～1.00 μg/kg and the limit of quantification (LOQ) was 1.0～2.0 μg/kg. This method was reliable for the determination of β-lactams in goat milk and other dietary products.
Shuaishuai, WANG; Xiaoping, ZHENG; Jin, MENG; and Yabin, HE
"Determination of β-lactams by ultra performance liquid chromatography-tandem mass spectrometry with QuEChERS in goat milk,"
Food and Machinery: Vol. 31:
3, Article 16.
Available at: https://www.ifoodmm.cn/journal/vol31/iss3/16
 王逸斌,徐莎,侯艳梅,等. 山羊奶的营养成分研究进展［J］.中国食物与营养, 2012, 18(10): 67～71.
 任洪辉,张鹏,王永. 我国奶羊产业发展的前景问题和对策［J］.江苏农业科学,2013, 41(11): 230～232.
 赵善仓,李增梅,董燕婕,等. 我国奶羊产业发展与羊乳质量安全现状及对策研究［J］.农产品质量与安全刊,2014（6）: 12～14.
 Kantiani Lina, Farré Marinella, Barceló Damiá, et al. Analytical methodologies for the detection of β-lactam antibiotics in milk and feed samples ［J］. Journal of TrAC Trends in Analytical Chemistry, 2009, 28(6): 729～744.
 Camara M, Gallego A, Garcinuno R M, et al. An HPLC—DAD method for the simultaneous determination of nine β-lactam antibiotics in ewe milk ［J］. Journal of Food Chemistry,2013(141): 829～834.
 Kukusamude Chunyapuk, Burakham Rodjana, Chailapakul Orawon, et al. High performance liquid chromato-graphy for the simultaneous analysis of penicillin residues in beef and milk using ion-paired extraction and binary water-acetonitrile mixture ［J］. Talanta, 2012, 92: 38～44.
 Li Na-si, Feng Feng, Yang Bing-cheng, et al. Simultaneous determination of beta-lactam antibiotics and beta-lactamase inhibitors in bovine milk by ultra performance liquid chromatography-tandem mass spectrometry ［J］.Journal of Chromatography B, 2014, 110(4): 945～946.
 刘洪斌,文一,刘勇军,等. LC—MS/MS法检测牛奶中14种β-内酰胺类抗生素残留［J］. 分析实验室, 2014, 33(2): 221～224.
 张秀尧, 蔡欣欣. 超高效液相色谱5三重四极杆质谱法快速同时测定牛奶中53种β-内酰胺类抗生素及其代谢产物的残留［J］.色谱, 2014,32(7): 693～701.
 陈瑞春, 贾海涛,艾连中,等. 高效液相色谱—串联质谱法测定牛奶和奶粉中9种青霉素类药物残留量［J］.食品科学, 2011, 32(18) : 249～252.
 黄百芬,吴丹青,蔡增轩,等. 超高效液相色谱—串联质谱法同时测定牛奶中19种β-内酰胺类抗生素［J］. 中国卫生检验杂志,2010,20(1): 1～6.
 Shiu-Hsieh Huan, His-Ya Huang, Szetsen Lee. Determination of eight penicillin antibiotics in pharmaceuticals, milk and porcine tissues by nano-liquid chromatography［J］. Journal of Chromatography A, 2009, 1 216(43): 7 186～7 194.
 李欣楠,韩镌竹,苏葳艺. 超高效液相色谱—串联质谱法同时测定生鲜乳中7类β-内酰胺类抗生素残留［J］.现代畜牧兽医,2013(11): 40～45.
 R Perez-Burgos, E M Grzelak, J Saurina, et al. Quechers methodologies as an alternative to solid phase extraction (SPE) for the determination and characterization of residues of cephalosporins in beef muscle using LC—MS/MS ［J］. Journal of Chromatography B, 2012(899): 57～65.