A method was developed for simultaneous determination of 8 β-lactams in goat milk by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLC—MS/MS). The milk was extracted with acetonitrile, purified with C18 and PSA sorbent and separated by an ACQUITY UPLC BEH C18 column using water (containing 0.1% formic acid) and acetonitrile as mobile phase. The mass spectrometric acquisitions were carried out by means of multiple reaction monitoring (MRM) in positive ionization mode. The linear relation of eight β-lactams was good and the correlation coefficient was more than 0.986. The recovery rates of the target analyte were between 83.8%~95.4%. The relative standard deviations were less than 6.5%. The limit of detection (LOD) of 8 β-lactams was 0.25~1.00 μg/kg and the limit of quantification (LOQ) was 1.0~2.0 μg/kg. This method was reliable for the determination of β-lactams in goat milk and other dietary products.

Publication Date


First Page


Last Page





[1] 王逸斌,徐莎,侯艳梅,等. 山羊奶的营养成分研究进展[J].中国食物与营养, 2012, 18(10): 67~71.
[2] 任洪辉,张鹏,王永. 我国奶羊产业发展的前景问题和对策[J].江苏农业科学,2013, 41(11): 230~232.
[3] 赵善仓,李增梅,董燕婕,等. 我国奶羊产业发展与羊乳质量安全现状及对策研究[J].农产品质量与安全刊,2014(6): 12~14.
[4] Kantiani Lina, Farré Marinella, Barceló Damiá, et al. Analytical methodologies for the detection of β-lactam antibiotics in milk and feed samples [J]. Journal of TrAC Trends in Analytical Chemistry, 2009, 28(6): 729~744.
[5] Camara M, Gallego A, Garcinuno R M, et al. An HPLC—DAD method for the simultaneous determination of nine β-lactam antibiotics in ewe milk [J]. Journal of Food Chemistry,2013(141): 829~834.
[6] Kukusamude Chunyapuk, Burakham Rodjana, Chailapakul Orawon, et al. High performance liquid chromato-graphy for the simultaneous analysis of penicillin residues in beef and milk using ion-paired extraction and binary water-acetonitrile mixture [J]. Talanta, 2012, 92: 38~44.
[7] Li Na-si, Feng Feng, Yang Bing-cheng, et al. Simultaneous determination of beta-lactam antibiotics and beta-lactamase inhibitors in bovine milk by ultra performance liquid chromatography-tandem mass spectrometry [J].Journal of Chromatography B, 2014, 110(4): 945~946.
[8] 刘洪斌,文一,刘勇军,等. LC—MS/MS法检测牛奶中14种β-内酰胺类抗生素残留[J]. 分析实验室, 2014, 33(2): 221~224.
[9] 张秀尧, 蔡欣欣. 超高效液相色谱5三重四极杆质谱法快速同时测定牛奶中53种β-内酰胺类抗生素及其代谢产物的残留[J].色谱, 2014,32(7): 693~701.
[10] 陈瑞春, 贾海涛,艾连中,等. 高效液相色谱—串联质谱法测定牛奶和奶粉中9种青霉素类药物残留量[J].食品科学, 2011, 32(18) : 249~252.
[11] 黄百芬,吴丹青,蔡增轩,等. 超高效液相色谱—串联质谱法同时测定牛奶中19种β-内酰胺类抗生素[J]. 中国卫生检验杂志,2010,20(1): 1~6.
[12] Shiu-Hsieh Huan, His-Ya Huang, Szetsen Lee. Determination of eight penicillin antibiotics in pharmaceuticals, milk and porcine tissues by nano-liquid chromatography[J]. Journal of Chromatography A, 2009, 1 216(43): 7 186~7 194.
[13] 李欣楠,韩镌竹,苏葳艺. 超高效液相色谱—串联质谱法同时测定生鲜乳中7类β-内酰胺类抗生素残留[J].现代畜牧兽医,2013(11): 40~45.
[14] R Perez-Burgos, E M Grzelak, J Saurina, et al. Quechers methodologies as an alternative to solid phase extraction (SPE) for the determination and characterization of residues of cephalosporins in beef muscle using LC—MS/MS [J]. Journal of Chromatography B, 2012(899): 57~65.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.