•  
  •  
 

Abstract

The bacterial community structure of three types Fen-Daqus (Qingcha Daqu, Houhuo Daqu and Hongxin Daqu) were studied through construction of bacteria 16S rRNA gene clone library, sequencing and phylogenetic analyses. The results showed that the bacteria communities of three types Fen-Daqus were both distributed in Bacilli, Actinobacteridae, Gammaproteobacteria. But there are some specific differences in detail composition of the bacterial species among the three types Fen-Daqus. Lactobacillus and Staphylococcus were the dominant bacteria in Qingcha Daqu (the ratio to total clones were 37.78% and 22.22% separately); Lactobacillus, Staphylococcus, Bacillus and Weissella were the dominant bacteria in Houhuo Daqu (the ratio to total clones were 35%, 16.67%, 16.67%, 13.33% separately); Lactobacillus, Bacillus and Thermoactinomyces were the dominant bacteria in Hongxin Daqu (the ratio to total clones were 26.67%, 28.89%, 17.78% separately).

Publication Date

6-28-2015

First Page

11

Last Page

15,73

DOI

10.13652/j.issn.1003-5788.2015.03.003

References

[1] 赵景龙. 浅谈清香型酒三种大曲的差异性[J]. 酿酒, 2000(5): 43~44.
[2] 沈怡方. 白酒生产技术全书[M]. 北京:中国轻工业出版社,2013.
[3] 都立辉, 刘芳. 16S rRNA基因在细菌菌种鉴定中的应用[J]. 乳业科学与技术, 2006(5): 207~209.
[4] 张守印, 郭学青, 李振军, 等. 16S rRNA基因克隆文库用于菌群分析的效能研究和评论[J]. 第三军医大学学报, 2008, 30(16): 1 549~1 552.
[5] 胡佳, 邓斌, 张学文. 浓香型白酒曲药中细菌组成及系统学分析[J].酿酒科技, 2007(5): 17~19.
[6] 陈希, 沈锡权, 翁佩芳, 等. 应用16S rDNA克隆文库法分析雪菜低盐腌制过程微生物群落的多样性[J]. 中国食品学报, 2012, 12(7): 205~210.
[7] 窦娜莎, 王琳. 16S rDNA克隆文库法分析Biostyr曝气生物滤池处理城市污水的细菌多样性研究[J]. 环境科学学报, 2011, 31(10):2 117~2 124.
[8] 黄晟, 陈旸, 潘雪莲, 等. 甘肃西峰黄土中细菌16S rDNA文库的构建与组成初步分析[J]. 高校地质学报, 2012, 18(4): 765~772.
[9] 吴谋成. 食品分析与感官评定[M]. 北京: 中国农业出版社, 2002.
[10] 叶光斌, 董瑞丽, 王彩虹, 等. 基于免培养法的中高温大曲细菌群落结构研究[J]. 江苏农业科学, 2013, 41(8): 333~336.
[11] Delong E F. Archaea in coastal marine environments[J]. Proc. Natl Acad Sci. USA, 1992, 89(12): 5 685~5 689.
[12] 叶光斌, 罗惠波, 杨晓东, 等. 基于免培养法研究泸州地区浓香型白酒窖泥原核微生物群落结构[J]. 食品科学, 2013, 34(17): 176~181.
[13] 叶光斌, 王风平, 肖湘. 东太平洋中国多金属结核区锰结核样品中微生物群落结构的研究[J]. 台湾海峡, 2010, 29(2): 218~227.
[14] 周恒刚. 制曲热能[J]. 酿酒科技, 2004(5): 35~37.
[15] 胡承, 邬捷锋, 沈才洪, 等. 浓香型(泸型)大曲的研究及其应用[J]. 酿酒科技, 2004(1): 33~36.
[16] 潘勤春, 孟镇, 钟其顶, 等. 汾酒大曲细菌群落结构的PCR—DGGE分析[J]. 酿酒科技, 2011(6): 95~99.
[17] 高亦豹. PCR—DGGE研究中国白酒大曲中微生物群落结构[D]. 无锡: 江南大学, 2010.
[18] Zhang X, Zhao J, Du X. Barcoded pyrosequencing analysis of the bacterial community of Daqu for light-flavour Chinese liquor[J]. Letters in Applied Microbiology, 2014, 58(6): 549~555.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.