Optimizeed the optimal conditions of Folin-Ciocalteucolorimetry, which was used to determine the total polyphenols content in sea buckthorn wine, and the sea buckthorn wine were investigated by orthogonal experiments combined with single factor experiments by using gallic acid as the standard. The results indicated that the total phenol contents of sea buckthorn wine could be well calculated according to their colorimetric absorption at 735 nm by applying Folin-Ciocalteu reagent 1.0 mL and 5 mL distilled water, 7.5%Na2CO3 3 mL, at 30 ℃ for colorimetric reaction, and there was a good linearity between content of phenol and absorbance in the range of 0~20 μg/mL. With this method, the stability, precision and average recovey rate were 1.46%, 0.22% and 2.63% by RSD (relative standard deviation), respectively. And the average recovery rate was 100.64%.This method is convenient and fast to determine the total polyphenols of sea buckthorn wine with the advantages of high precision, stability and good repetition.

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[1] 李淑珍, 武飞, 陈月林, 等. 沙棘活性成分及功效研究进展[J]. 中国民族民间医药, 2015, 23(1): 51-53.
[2] 鲁长征, 山永凯, 杨莉华, 等. 沙棘的研究及其开发应用[J]. 中国食物与营养, 2010, 20(3): 26-28.
[3] 李琼, 陈恺, 陈燕勤, 等. 大孔吸附树脂分离纯化核桃青皮总多酚[J]. 食品与机械, 2015, 31(1): 175-180.
[4] 赵二劳, 韩永花. 沙棘的营养保健功能与开发利用[J]. 食品研究与开发, 2005, 26(4): 182-184.
[5] 李峰, 刘浩, 钟媛, 等. 沙棘叶中总多酚和总黄酮的提取工艺[J]. 食品与机械, 2012, 28(4): 128-130.
[6] 牛广财, 朱丹, 王军, 等. 沙棘酒清除自由基作用的研究[J]. 中国食品学报, 2010, 10(1): 36-41.
[7] 李春阳, 许时婴, 王璋. 低浓度香草醛—盐酸法测定葡萄籽中原花青素含量的研究[J]. 食品工业科技, 2004, 25(6): 128-130.
[8] 肖纯, 张凯农, 阳长敏, 等. 铁氰化钾分光光度法测定单宁的适用性[J]. 华北农学报, 1996, 11(2): 127-130.
[9] Silvina B L, Balz F. Relevance of apple polyphenols as antioxidants in human plasma: Contrasting in vitro and in vivo effects [J]. Free Radical Biology & Medicine, 2004, 36(2): 201-211.
[10] Neuza Paixao, Rosa Perestrelo, Jose C Marques, et al. Relationship between antioxidant capacity and total phenolic content of red, rose and white wines[J]. Food Chemistry, 2007, 101(1): 204-214.
[11] 徐佳, 辛立方, 张瑞廷, 等. 改良的Folin-Ciocalteu比色法测定核桃外果皮中总多酚含量[J]. 食品工业科技, 2012, 33(6): 60-63.
[12] 李静, 聂继云, 王孝娣, 等. Folin-Ciocalteus法测定葡萄和葡萄酒中的总多酚[J]. 中国南方果树, 2007, 36(6): 86-87.
[13] 王皎, 高颖, 赵艳敏, 等. 测定不同品种苹果果皮和果肉中总多酚的含量[J]. 食品研究与开发, 2013, 34(20): 87-89.
[14] 朱霞, 李焕荣, 罗游. Folin-Ciocalteu比色法测定核桃青皮中多酚含量条件的优化[J]. 食品与机械, 2014, 30(4): 122-125.
[15] 田桂芝, 李绍明, 王大超, 等. Folin-Ciocalteu比色法测定板栗总苞提取液中总多酚的含量[J]. 安徽农业科学, 2015, 43(6): 289-291, 370.
[16] 李颖畅, 吕艳芳, 励建荣. Folin-Ciocalteu法测定不同品种蓝莓叶中多酚含量[J]. 中国食品学报, 2014, 14(1): 273-278.
[17] 范琼, 周聪, 赵敏, 等. Folin-Ciocalteu比色法测定树仔菜总多酚[J]. 南方农业学报, 2014, 45(12): 2 230-2 235.



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