•  
  •  
 

Abstract

The effects of methanol, ethanol and acetone was investigated, on the extraction of phenolic contents and antioxidant activity from oil-tea meal under hot reflux and ultrasonic assistances. Under hot reflux and ultrasonic assistances, phenolic contents of acetone extracts were 1 444 ± 2 and 1 623 ± 1 mg GAE / 100 g (DW), respectively. that were significantly higher (P<0.05) than the corresponding methanol and ethanol extracts. Phenolic compounds, including catechin, epicatechin, rutin and quercetin were determinate by HPLC in these extracts. Under hot reflux and ultrasonic assistances, the ORAC values of acetone extracts were 358.4 ± 16.9 and 370.6 ± 42.1 μmol TE/ g (DW), respectively. That were significantly higher (P<0.05) than the corresponding methanol and ethanol extracts. These results show that the extraction efficiency of acetone coupled with ultrasonic is better than other methods.

Publication Date

4-28-2016

First Page

172

Last Page

175,186

DOI

10.13652/j.issn.1003-5788.2016.04.040

References

[1] 朱勇. 茶油的脂肪酸变化及其生物酚的抗氧作用[D]. 长沙: 中南林业科技大学, 2013: 1.
[2] 龚吉军, 钟海雁, 黄卫文, 等. 油茶粕多肽降血脂活性研究[J]. 食品研究与开发, 2012(10): 24-28.
[3] 方飞, 王力生. 油茶粕资源综合开发利用研究进展[J]. 饲料博览, 2011(6): 30-32.
[4] 刘尧刚, 胡健华, 周易枚. 油茶粕中茶皂素提取工艺优化的研究[J]. 粮油加工, 2008(6): 80-83.
[5] 刘小如, 张丽美, 胡蒋宁, 等. 油茶粕多糖的分级纯化及结构研究[J]. 食品科学, 2013(23): 96-102.
[6] 龚吉军, 黄卫文, 钟海雁, 等. 油茶粕多肽对小鼠免疫调节功能的影响[J]. 中国食品学报, 2013(12): 21-27.
[7] 康海权, 陈亚琪, 陈秋平, 等. 油茶果壳多糖的抗氧化作用及单糖组成[J]. 食品与发酵工业, 2010(4): 36-39.
[8] 张宽朝, 马皖燕, 文汉. 油茶籽多糖降血糖作用的初步研究[J]. 食品工业科技, 2014(2): 337-339.
[9] 柳荣祥, 朱全芬, 夏春华. 茶皂素生物活性应用研究进展及发展趋势[J]. 茶叶科学, 1996(2): 3-8.
[10] 郝卫宁, 曾勇, 胡美英, 等. 茶皂素在农药领域的应用研究进展[J]. 农药, 2010, 49(2): 90-93, 96.
[11] 袁英姿, 曹清明, 钟海雁, 等. 油茶籽多酚对茶油的抗氧化性的研究[J]. 食品工业科技, 2009(6): 103-105.
[12] 侍银宝, 钟海雁. 油茶果皮多酚粗提物对DNA氧化损伤的影响[J]. 食品与机械, 2013, 29(6): 143-146.
[13] 王徐卿, 沈建福, 王敏, 等. 油茶籽饼多酚的乙醇提取工艺优化研究[J]. 浙江林业科技, 2007, 27(3): 41-44.
[14] 曹耀强, 谢新华, 陈卫军, 等. 油茶饼中酚类化合物的抗氧化性研究[J]. 食品科技, 2012(3): 201-204.
[15] 宋立霞, 王向社, 李明芳, 等. 氧自由基吸收能力测定方法的研究进展[J]. 食品研究与开发, 2008, 29(12): 166-170.
[16] 王玉春. 茶多酚的提取方法及应用研究进展[J]. 甘肃联合大学学报: 自然科学版, 2008, 22(3): 51-55.
[17] Zhao Hai-feng, Dong Jian-jun, Lu Jian, et al. Effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley (Hordeumvulgare L.) [J]. Journal of Agricultural and Food Chemistry, 2006, 54(19): 7 277-7 286.
[18] 李志西, 李元瑞, 葛蕾, 等. 苹果渣多酚超声波提取工艺及其抗氧化性研究[J]. 西北农林科技大学学报: 自然科学版, 2005, 33(8): 131-134.
[19] 蒋丽, 王雪梅, 全学军, 等. 不同提取方法对茶多酚理化性质的影响[J]. 食品科学, 2010(14): 136-139.
[20] 谢娇阳, 何志鹏, 谢东养, 等. 超声辅助乙醇提取油茶籽饼多酚工艺的优化[J]. 南方农业学报, 2012, 43(4): 515-519.
[21] Singleton V L, Rossi J A. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents[J]. American journal of Enology and Viticulture, 1965, 16(3): 144-158.
[22] 廖义秀, 钟海雁. 茶油贮藏的稳定性及高效液相色谱分析[J]. 食品与机械, 2013, 29(1): 173-176.
[23] Huang De-jian, Ou Bo-xin, Hampsch-Woodill M, et al. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format[J]. Journal of Agricultural and Food Chemistry, 2002, 50(16): 4 437-4 444.
[24] 白雪莲. 苹果渣多酚分离鉴定与体外抗氧化和抗炎活性初步研究[D]. 杨凌: 西北农林科技大学, 2011: 18-22.
[25] 陆健, 孔维宝, 赵海峰, 等. 大麦总多酚不同溶剂提取物对DPPH自由基清除能力的影响[J]. 食品与生物技术学报, 2008, 27(1): 58-61.
[26] 董建瑞, 黄阿根, 梁文娟. 茶树花多酚提取工艺的研究[J]. 食品与机械, 2007, 23(1): 83-86.
[27] 刘红, 李元元, 龚晓武, 等. 不同提取方法对沙枣多酚清除自由基能力的比较研究[J]. 食品科技, 2010, 35(11): 227-230.
[28] 邓义书, 包海蓉, 齐知耕. 桑葚果渣中总多酚的不同提取方法比较研究[J]. 湖南农业科技, 2010(15): 106-108.
[29] Prior R L, Hoang H, Gu Li-wei, et al. Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity(ORAC)) of plasma and other biological and food samples [J]. Journal of Agricultural and Food Chemistry, 2003, 51(11): 3 273-3 279.
[30] 徐维盛, 李东, 刘静, 等. ORAC法对12种水果总抗氧化能力评价研究[J]. 食品工业, 2014, 35(1): 247-250.
[31] 赵建, 宋亮楠, 刘薇, 等. ORAC法测定保健食品抗氧化能力的体内外实验对比分析[J]. 食品科学, 2011, 32(15): 103-108.
[32] 徐维盛, 张桂雨, 刘静, 等. ORAC法评价16种茶叶的抗氧化能力[J]. 食品安全质量检测学报, 2014, 5 (1): 241-246.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.