Abstract
Brewer’s grains peptide SS-5(sequence: Ser-Pro-Asp-Arg-Ser) was synthesized by solid-phase synthesis with Fmoc protect group. Then the crude synthetic peptide was purified and desalted by HPLC, and as a result the purity was over 95%. The inhibitory activity of synthetic peptide SS-5 to α-glucosidase was detected, and the results showed that it was influenced by salt ionic concentration and the lower concentration presented higher inhibility. Furthermore, the interaction between synthetic peptide SS-5 and α-glucosidase was confirmed by circular dichroism spectrum(CD) and fluorescence quenching assays.. Therefore, a fast method of obtaining brewer’s grains peptide SS-5 with α-glucosidase inhibitory activity in quantity was proposed in this paper.
Publication Date
8-28-2016
First Page
1
Last Page
5
DOI
10.13652/j.issn.1003-5788.2016.08.001
Recommended Citation
Yuwen, FANG; Jinhong, WU; Zhengwu, WANG; Li, GAN; Jiajun, HE; Shanshan, CHEN; Hongcheng, MA; and Haiming, SHI
(2016)
"Synthesis and characterization of brewer’s grains peptide with α-glucosidase inhibitory activity,"
Food and Machinery: Vol. 32:
Iss.
8, Article 1.
DOI: 10.13652/j.issn.1003-5788.2016.08.001
Available at:
https://www.ifoodmm.cn/journal/vol32/iss8/1
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