A method was developed screening, confirming and quantifying multiple additives and banned dyes in wine, juice and other beverages by liquid chromatography-ion trap-time of flight tandem mass spectrometry (LC-MS-IT-TOF). The accurate and multiple-stage mass spectrometry database of 41compounds was established. The analytes in samples were diluted with water-methanol (v/v). In the chromatographic analysis, target compounds were separated on a C18 column (150 mm × 4.6 mm, 5 μm) with the gradient elution using the mobile phases of methanol and 2 mM ammonium acetate. The results showed that the limits of detection were 0.005~1.0 mg/kg. The method validation was carried out at three spiking levels, and the recoveries were 41.2%~114.1%, with the relative standard deviations (RSDs) of 4.3%~16.4%. The screening of analytes was performed by precision mass matching and library searching. The retention time, isotopic abundance and multiple-stage ion mass spectral was employed in this confirmation. This method is simple, fast, credible and highly sensitive and can be applied to screening both multiple and illegal additives in foods without reference standards, reducing the analytical time and improving detection efficiency.

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[1] 景顺杰, 李建晴. 分光光度法直接测定饮料中的柠檬黄和胭脂红[J]. 山西大同大学学报: 自然科学版, 2007, 23(3): 22-24.
[2] 王冬妍, 杨晓丽, 李兴权, 等. 高效液相色谱法测定黄桃罐头中的 8 种合成着色剂[J]. 理化检验: 化学分册, 2010, 46(9): 1 034.
[3] 李帮锐, 冯家力, 潘振球, 等. 高效液相色谱—质谱/质谱联用法测定饮料中的人工合成色素[J]. 中国卫生检验杂志, 2007, 17(4): 579-581.
[4] 王丽芳, 丁晓静, 解娜, 等. 毛细管电泳法测定糖果及调制酒中10种人工合成色素[J]. 食品科学, 2014, 35(14): 145-150.
[5] XUE Yong, CHEN Na, LUO Chun-ying, et al. Simultaneous determination of seven preservatives in cosmetics by dispersive liquid-liquid microextraction coupled with high performance capillary eletrophoresis [J]. Anal Methods, 2013(5): 2 391-2397.
[6] 张继红, 周兴旺, 杨代明, 等. 油脂及油炸食品中四种抗氧化剂的同时测定[J]. 食品与机械, 2010, 26(3): 79-82.
[7] KAMANKESH M, MOHAMMADI A, TEHRANI Z M, et al. Dispersive liquid-liquid chromatography for determination of benzoate and sorbate in yogurt drinks and method optimization by central composite design [J]. Talanta, 2013, 109: 46-51.
[8] 刘红河, 陈卫, 康莉, 等. 气相色谱法同时测定食品中3种抗氧化剂[J]. 现代预防医学, 2006, 33(12): 2 307
[9] 陈志涛, 张睿, 郑香平, 等. 离子色谱法测定葡萄酒中的山梨酸和苯甲酸的含量[J]. 理化检验: 化学分册, 2015, 51(12): 1 658-1 660.
[10] 向俊, 徐建双, 毛丽秋. 液相微萃取/气相色谱—质谱法分析食品中的防腐剂和抗氧化剂[J]. 分析科学学报, 2010, 26(2): 187-190.
[11] 宋瑞霞, 谢翔燕. 毛细管气相色谱法测定食品中的甜蜜素[J]. 食品研究与开发, 2008, 29(1): 128-130.
[12] 杨柳桦, 王林, 孙成均. 高效液相色谱法测定保健食品和饮料中的阿斯巴甜[J]. 分析试验室, 2007, 26(7): 79-80.
[13] 嵇超, 冯峰, 陈正行, 等. 高效液相色谱—串联质谱法测定葡萄酒中的 5 种人工合成甜味剂[J]. 色谱, 2010, 28(8): 749-753.
[14] 王美玲, 戴洁芸, 成婧, 等. 分散固相萃取/高效液相色谱—离子阱飞行时间高分辨质谱法对纺织品中全氟化合物的快速筛查和确证[J]. 分析测试学报, 2016, 35(3): 257-263.



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