Application of ionic liquids in the detection of antibiotic negative milk

Authors

ZHOU Xinkui, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Gansu Zhongshang Food Quality Test and Detection Co.,Ltd., Lanzhou, Gansu 730010, China
HONG Xia, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Gansu Zhongshang Food Quality Test and Detection Co.,Ltd., Lanzhou, Gansu 730010, China
QIAN Yingwen, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Gansu Zhongshang Food Quality Test and Detection Co.,Ltd., Lanzhou, Gansu 730010, China
DENG Lijuan, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Gansu Zhongshang Food Quality Test and Detection Co.,Ltd., Lanzhou, Gansu 730010, China
WANG Jiebin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Gansu Zhongshang Food Quality Test and Detection Co.,Ltd., Lanzhou, Gansu 730010, China
GAO Zhiying, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Gansu Zhongshang Food Quality Test and Detection Co.,Ltd., Lanzhou, Gansu 730010, China
HE Haining, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Gansu Zhongshang Food Quality Test and Detection Co.,Ltd., Lanzhou, Gansu 730010, China
LIU Qi, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Gansu Zhongshang Food Quality Test and Detection Co.,Ltd., Lanzhou, Gansu 730010, China

Abstract

Hydrophobic ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate [(C₈MIM) (PF₆)] was synthesized using metathesis method, and then the ionic liquid was purified by activated carbon and macroporous resin solid phase extraction column, to get a colorless, transparent product [(C₈MIM) (PF₆)] with a yield of 87.6%. The ionic liquids was used to extract penicillin and penicillin-thiazole acid hydrolysates in milk and dairy products, and its extracting condition was optimized. After that the content of penicillin and penicillin thiazole acid were detected by utilizing self-developed HPLC method, and it could be indirectly determined whether the milk and dairy products were true antibiotic negative milk. In this study, the recovery rate of penicillin by ionic liquid extraction method was 87.9%～92.6%, with RSD 1.8%, and the detection limit was 5 ng/mL. Moreover, the recovery rate of penicillin thiazole acid was found to be 83.5%～90.8%, with RSD 2.4%, and the detection limit was also 5 ng/mL. Compared with both plate and liquid chromatography mass spectrometry methods, our method here is more predominant.

Publication Date

8-28-2016

First Page

63

Last Page

66

DOI

10.13652/j.issn.1003-5788.2016.08.015

Recommended Citation

Xinkui, ZHOU; Xia, HONG; Yingwen, QIAN; Lijuan, DENG; Jiebin, WANG; Zhiying, GAO; Haining, HE; and Qi, LIU (2016) "Application of ionic liquids in the detection of antibiotic negative milk," Food and Machinery: Vol. 32: Iss. 8, Article 15.
DOI: 10.13652/j.issn.1003-5788.2016.08.015
Available at: https://www.ifoodmm.cn/journal/vol32/iss8/15

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