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Abstract

In this study, spoilage bacteria in fresh Xiang lotus seeds preserved at 4 ℃ temperature for 18 d were separated by dilution-plate method. Total genomic DNA was extracted from these strains and then subjected to PCR amplification. Dominant spoilage bacteria were identified using 16S rDNA sequencing for the identification and the law of their growth and declination was drawn. The results showed the four dominant spoilage bacteria in fresh Xiang lotus seeds preserved at the temperature of 4 ℃ were Pantoea dispersa, Staphylococcus epidermidis, Staphylococcus warneri, and Pantoea agglomerans. At the storage temperature of 4 ℃, the growing trends of Pantoea dispersa, Staphylococcus epidermidis, Staphylococcus warneri of fresh lotus seeds were similar, which all needed an adaptive phase before their increase. The amount change of Pantoea agglomerans was irregular.

Publication Date

8-28-2016

First Page

107

Last Page

109,139

DOI

10.13652/j.issn.1003-5788.2016.08.026

References

[1] 郑天闻, 徐婷婷, 范亚苇, 等. 莲子多酚氧化酶的酶学性质[J]. 食品工业科技, 2014, 35(2): 162-165.
[2] BHAT R, SRIDHAR K R. Nutritional quality evaluation of electron beam-irradiated lotus (Nelumbo nucifera) seeds[J]. Food Chemistry, 2008, 107(1): 174-184.
[3] WU Jin-zhong, ZHENG Yuan-bin, CHEN Ti-qiang, et al. Evaluation of the quality of lotus seed of Nelumbo nucifera Gaertn from outer space mutation[J]. Food Chemistry, 2007, 105(2): 540-547.
[4] 王亮亮, 夏延斌, 任美. 湘莲的营养和保健功效及其在食品加工中的应用[J]. 食品与机械, 2015, 31(2): 262-266.
[5] 王建辉, 靳娜, 刘永乐, 等. 低温处理对湘莲采后生理变化的影响[J]. 食品科学, 2014, 35(18): 209-213.
[6] TOURNAS V H. Moulds in fresh and minimally processed vegetables and sprouts[J]. International Journal of Food Microbiology, 2005, 99(1): 71-77.
[7] 范贤贤, 田密霞, 姜爱丽, 等. 鲜切果蔬表面微生物侵染途径及控制[J]. 保鲜与加工, 2009(2): 14-16.
[8] 易俊敖. 莲子的贮藏技术[J]. 长江蔬菜, 1992(2): 38-39.
[9] MIGNARD S, FLANDROIS J P. 16S rRNA sequencing in routine bacterial identification: a 30-month experiment[J]. Journal of Microbiological Methods, 2006, 67(3): 574-581.
[10] 杨静静, 孟镇, 钟其顶, 等. 分子生物学技术在酵母菌多相分类鉴定中的应用[J]. 中国酿造, 2011(4): 16-19.
[11] LEI Zhang-yu, ZHANG Xiao. Application of 16S rDNA sequence analysis technique in microbial detection[J]. Agricultural Science&Technology, 2014, 15(4): 520-523.
[12] PHILIPP S, HUEMER H P, IRSCHICK E U, et al. Obstacles of multiplex real-time PCR for bacterial 16S rDNA: primer specifity and DNA decontamination of Taq polymerase[J]. Transfusion Medicine & Hemotherapy, 2010, 37(1): 21-28.
[13] KIERZKOWSKA M, MAJEWSKA A, KUTHAN R T, et al. A comparison of Api 20A vs MALDI-TOF MS for routine identification of clinically significant anaerobic bacterial strains to the species level[J]. Journal of Microbiological Methods, 2013, 92(2): 209-212.
[14] 郭全友, 杨宪时, 许钟. 冷藏罗非鱼优势腐败菌的鉴定及其特征[J]. 食品与机械, 2009, 25(3): 87-90.
[15] SUN Shan, FAN Xin-xing, PHARMACY D O, et al. RAPD-PCR and 16S rDNA phylogenetic analysis of alkaline protease producing bacteria isolated from soil of India: Identification and detection of genetic variability[J]. Journal of Genetic Engineering & Biotechnology, 2014, 12(1): 27-35.
[16] CHANG Shu-chen, KUND H F, CHEN H C, et al. Determination of histamine and bacterial isolation in swordfish fillets (Xiphias gladius) implicated in a food borne poisoning[J]. Food Control, 2008, 19(1): 16-21.
[17] 韩永和, 章文贤, 庄志刚, 等. 耐盐好氧反硝化菌A-13菌株的分离鉴定及其反硝化特性[J]. 微生物学报, 2013, 53(1): 47-58.
[18] 王满生. 多靶栅栏技术在草鱼贮藏过程中对微生物腐败的控制研究[D]. 长沙: 长沙理工大学, 2012: 21-34.

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