Establishment of arapid detection method for carbendazim enzyme-linked immunosorbent

Authors

JIANG Wen-hui, Hangzhou People's Procuratorate of Zhejiang Province, Hangzhou, Zhejiang 310012, China
WU Xiao-sheng, Beijing Kwinbon Biotechnology Company, Beijing 102206, China; Beijing Engineering Research Centre of Food Safety Immunodetection, Beijing 102206, China
CUI Na, Beijing Kwinbon Biotechnology Company, Beijing 102206, China; Beijing Engineering Research Centre of Food Safety Immunodetection, Beijing 102206, China
ZHANG Yu, Beijing Kwinbon Biotechnology Company, Beijing 102206, China; Beijing Engineering Research Centre of Food Safety Immunodetection, Beijing 102206, China
PENG Zheng-xue, Beijing Wanger Biotechnology Company, Beijing 102206, China
CUI Hai-feng, Beijing Kwinbon Biotechnology Company, Beijing 102206, China; Beijing Engineering Research Centre of Food Safety Immunodetection, Beijing 102206, China
WAN Yu-ping, Beijing Kwinbon Biotechnology Company, Beijing 102206, China; Beijing Engineering Research Centre of Food Safety Immunodetection, Beijing 102206, China

Abstract

Using the principle of enzyme-linked immunosorbent assay, a method for quantitative determination of carbendazim residues in fruits, vegetables, tea, and tobacco was established. First, the carbendazim hapten was prepared through acid-base neutralization chemical reaction, and then the anti-carbendazim monoclonal antibody was screened out by immunizing mice to prepare the enzyme-labeled goat anti-mouse anti-antibody, and finally the prepared anti-carbendazim Monoclonal antibodies and enzyme-labeled goat anti-mouse anti-antibodies were used in the development of enzyme-linked ELISA kits. The results showed that within the linear range of 0.1~8.1 μg/L, the target carbendazim had a good linear relationship, and the correlation coefficient (R²) could reach 0.995. The recoveries of the kits at the addition levels of 300, 600, and 1 200 μg/kg were 76.0%~102.2%, and the relative standard deviations were all less than 10%. The kit detected carbendazim in tobacco leaves, apples, cabbage, and tea samples, and the detection limits were 266.3, 421.0, 349.1, and 484.4 μg/kg (n=20). This method was a rapid detection method and suitable for the determination of carbendazim residues in fruits, vegetables, tea, and tobacco leaves.

Publication Date

1-28-2021

First Page

94

Last Page

98

DOI

10.13652/j.issn.1003-5788.2021.01.014

Recommended Citation

Wen-hui, JIANG; Xiao-sheng, WU; Na, CUI; Yu, ZHANG; Zheng-xue, PENG; Hai-feng, CUI; and Yu-ping, WAN (2021) "Establishment of arapid detection method for carbendazim enzyme-linked immunosorbent," Food and Machinery: Vol. 37: Iss. 1, Article 14.
DOI: 10.13652/j.issn.1003-5788.2021.01.014
Available at: https://www.ifoodmm.cn/journal/vol37/iss1/14

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