Objective: This study aimed to develop the application of egg yolk powder in the field of feed, functional food and biopharmaceuticals. Methods: Using fresh eggs as raw materials, yolk powder was prepared by spray drying, and the effects of different parameters on the content of yolk antibody (IgY), protein structure and functional properties of egg yolk powder were analyzed. Results: Considering the antibody content, physicochemical properties and functional properties of egg yolk powder comprehensively, when the inlet air temperature was 140 ℃ and the feed flow rate was 600 mL/h, the IgY content of egg yolk powder was 17.71 mg/g, and the solubility was 61.97% respectively. The powdery rate and water content was 36.46% and 3.8% respectively, and the surface hydrophobicity, water holding capacity and oil holding capacity was 106.67 μg, 3.63 g/g and 3.03 g/g respectively. The emulsifying property was 27.15%, and the emulsifying stability was 24.48%. The overall quality isoptimum. Conclusion: Changing the spray drying parameters can keep the structure of egg yolk powder intact and improve the physicochemical and functional properties of egg yolk powder, as well as ensure the high IgY content.

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[1] 张江.鸡蛋改善人类健康的研究和技术新进展(综述)[J].国外畜牧学(猪与禽),2018,38(8):62-65.ZHANG J.What's new in chicken egg research and technology for human health promotion?:A review[J].Foreign Animal Husbandry(Swine and Poultry),2018,38(8):62-65.
[2] KOVACS-NOLAN J,MINE Y.Egg yolk antibodies for passive immunity[J].Annu Rev Food Sci Technol,2012,3:163-182.
[3] 安芳兰,李菁,张荣,等.卵黄抗体在疾病防治中应用的研究进展[J].安徽农业科学,2016,44(5):68-71.AN F L,LI J,ZHANG R,et al.Research progress of egg yolk antibody in the prevention and treatment of various diseases[J].Journal of Anhui Agri,2016,44(5):68-71.
[4] 亓秀晔,宋翔,王业华,等.卵黄抗体在疾病防制中的应用研究进展[J].中国畜牧兽医,2017,44(9):2 767-2 772.QI X Y,SONG X,WANG Y H,et al.Research progress on immunoglobulin of yolk in the prevention and treatment of various diseases[J].Chinese Animal Husbandry & Veterinary Medicine,2017,44(9):2 767-2 772.
[5] SANE S U,WONG R,HSU C C.Raman spectroscopic characterization of drying-induced structural changes in a therapeutic antibody:Correlating structural changes with long-term stability[J].Journal of Pharmaceutical Sciences,2004,93(4):1 005.
[6] MAHLER H C,MULLER R,FRIESS W,et al.Induction and analysis of aggregates in a liquid IgG1-antibody formulation[J].Eur J Pharm Biopharm,2005,59(3):407-417.
[7] VAILLANT A A J.Use of dot blots analysis in the separation of anti-HIV antibodies in animals[J].Journal of Chromatography & Separation Techniques,2013,4(5):1 805-1 812.
[8] 要志宏,关倩倩,聂相珍,等.食品干燥技术研究进展[J].农业与技术,2016,36(16):249-250.YAO Z H,GUAN Q Q,NIE X Z,et al.Research progress of food drying technology[J].Agriculture and Technology,2016,36(16):249-250.
[9] 刘静波,马爽,林松毅,等.速溶蛋黄粉喷雾干燥工艺优化及其特性[J].吉林大学学报(工学版),2012,42(5):1 336-1 342.LIU J B,MA S,LIN S Y,et al.Optimum spray-drying technology and properties of instant egg yolk powder[J].Journal of Jilin University(Engineering and Technology Edition),2012,42(5):1 336-1 342.
[10] 王清平,彭志伟,刘晓婷,等.蛋黄粉生产工艺优化研究[J].湖南饲料,2021(2):37-39.WANG Q P,PENG Z W,LIU X T,et al.Research on optimization of production process of egg yolk powder[J].Hunan Feed,2021(2):37-39.
[11] 程方圆.鸡蛋液喷雾干燥工艺参数优化研究[D].天津:天津农学院,2019:22-32.CHENG F Y.Optimization of process parameters of egg liquid spray drying[D].Tianjin:Tianjin Agricultural University,2019:22-32.
[12] 陈珂,刘丽莉,孟圆圆,等.鸡蛋清蛋白喷雾干燥工艺优化及特性变化[J].食品与机械,2019,35(1):197-203,231.CHEN K,LIU L L,MENG Y Y,et al.Optimization of egg white protein spray drying process and its characteristics[J].Food & Machinery,2019,35(1):197-203,231.
[13] SHEN H,ZHAO M M,SUN W Z.Effect of pH on the interaction of porcine myofibrillar proteins with pyrazine compounds[J].Food Chemistry,2019,287:93-99.
[14] HADNADEV M,DAPCEVIC-HADNACEV T,LAZARIDOU A,et al.Hempseed meal protein isolates prepared by different isolation techniques Part I:Physicochemical properties[J].Food Hydrocolloids,2018,79:526-533.
[15] 王家镔,林军,曲可欣,等.喷雾干燥与冷冻干燥鸡蛋粉特性研究[J].食品科技,2020,45(3):80-86.WANG J B,LIN J,QU K X,et al.Comparison of microstructure and physicochemical propertiess of spray drying and freeze-dried egg powder[J].Food Science and Technology,2020,45(3):80-86.
[16] 谭佩毅,黄秀锦.卵黄抗体鸡蛋粉喷雾干燥工艺研究[J].食品工业科技,2012,33(20):269-272,275.TAN P Y,HUANG X J.Preparation of yolk immunoglobulin-rich egg powder by spray-drying[J].Science and Technology of Food Industry,2012,33(20):269-272,275.
[17] 夏轩泽,李言,钱海峰,等.改性处理对豌豆蛋白结构和功能特性的影响[J].食品科学技术学报,2021,39(5):32-38,48.XIA X Z,LI Y,QIAN H F,et al.Effects of modification treatment on the structure and functional properties of pea protein[J].Journal of Food Science and Technology,2021,39(5):32-38,48.
[18] RAO Q,LABUZA T P.Effect of moisture content on selected physicochemical properties of two commercial hen egg white powders[J].Food Chem,2012,132(1):373-384.
[19] RANNOU C,QUEVEAU D,BEAUMAL V,et al.Effect of spray-drying and storage conditions on the physical and functional properties of standard and n-3 enriched egg yolk powders[J].Journal of Food Engineering,2015,154:58-68.
[20] ULRICHS T,DROTLEFF A M,TERNES W.Determination of heat-induced changes in the protein secondary structure of reconstituted livetins(water-soluble proteins from hen's egg yolk)by FTIR[J].Food Chem,2015,172:909-920.
[21] GUAN J J,ZHANG T B,HUI M,et al.Mechanism of microwave-accelerated soy protein isolate-saccharide graft reactions[J].Food Research International,2011,44(9):2 647-2 654.
[22] ELLEPOLA S W,CHOI S M,MA C Y.Conformational study of globulin from rice(Oryza sativa)seeds by Fourier-transform infrared spectroscopy[J].Int J Biol Macromol,2005,37(1/2):12-20.
[23] HOU D H J,CHANG S K C.Structural characteristics of purified glycinin from soybeans stored under various conditions[J].Journal of Agricultural and Food Chemistry,2004,52(12):3 792-3 800.
[24] 曾琪,胡淼,王欢,等.pH值处理对黑豆分离蛋白结构、流变特性及乳化性能的影响[J].食品科学,2020,41(22):15-21.ZENG Q,HU M,WANG H,et al.Effect of pH treatment on structure,rheological properties and emulsifying properties of black bean protein isolate[J].Food Science,2020,41(22):15-21.
[25] JIANG S,DING J,ANDRADE J,et al.Modifying the physicochemical properties of pea protein by pH-shifting and ultrasound combined treatments[J].Ultrasonics Sonochemistry,2017,38:835-842.
[26] LI Q Q,ZHANG X Y,TANG S T,et al.Improved effect of ultrasound-assisted enzymolysis on egg yolk powder:Structural properties,hydration properties and stability characteristics[J].Food Chemistry,2022,382:132549.
[27] KEERATI-U-RAI M,CORREDIG M.Heat-induced changes in oil-in-water emulsions stabilized with soy protein isolate[J].Food Hydrocolloids,2009,23(8):2 141-2 148.
[28] KIOSSEOGLOU V.Egg yolk protein gels and emulsions[J].Current Opinion in Colloid & Interface Science,2003,8(4/5):365-370.



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