Objective:This study aimed to effectively improve the risk warning and quality and safety supervision of polycyclic aromatic hydrocarbons (PAHs) in spicy strip.Methods: The target compounds in the samples were extracted with acetonitrile and purified by QuEChERS, containing 100 mg N-propyl ethylenediamine (PSA), 100 mg octadecylsilane bonded silica gel (C18), and 1 000 mg neutral alumina adsorbent, and then were separated by DB-EUPAH column. It was detected by gas chromatography-triple quadrupole mass spectrometer with multiple reaction monitoring mode and matrix matching external standard method.Results: The 16 kinds of PAHs showed good linearity in the range of 0.005~0.500 μg/mL, and the correlation coefficients were 0.998 4~0.999 9. The limits of detection and quantification were 0.04~0.55 μg/kg and 0.12~1.85 μg/kg, respectively. The average recoveries of 16 kinds of PAHs were 71.54%~103.70% with the relative standard deviations of 2.4%~9.3% at three-level spiked standard sample of 10, 20 and 100 μg/kg. The method was used to analyze the contamination of 16 kinds of PAHs in 20 batches of spicy strip. PAHs were detected in 14 batches with the content ranging from 0.42 μg/kg to 42.28 μg/kg.Conclusion: The method is easy to operate with high sensitivity, and suitable for daily detection of 16 kinds of PAHs in spicy strip.

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[1] PURCARO G,MORET S,CONTE L S.Overview on polycyclic aromatic hydrocarbons:Occurrence,legislation and innovative determination in foods[J].Talanta,2013,105:292-305.
[2] 冯丽.离心平行蒸发—气相色谱/质谱法同时测定土壤中多环芳烃和硝基苯类化合物[J].色谱,2020,38(5):617-619.FENG Li.Simultaneous determination of Polycyclic aromatic hydrocarbon and nitrobenzene compounds in soil by gas chromatography-mass spectrometry with centrifugal parallel evaporation[J].Chinese Journal of Chromatography,2020,38(5):617-619.
[3] 岳中慧,吴亮,张皓.快速溶剂萃取—气相色谱—质谱法同时测定土壤中多环芳烃、六六六和滴滴涕[J].化学分析计量,2021,30(3):49-55.YUE Zhong-hui,WU Liang,ZHANG Hao.Simultaneous determination of PAHs,BHC and DDT in soil by using accelerated solvent extraction and GC-MS[J].Chemical Analysis and Meterage,2021,30(3):49-55.
[4] LEDESMA E,RENDUELES M,DÍAZ M.Contamination of meat products during smoking by polycyclic aromatic hydrocarbons:Processes and prevention[J].Food Control,2016,60:64-87.
[5] CHEN Hong-ping,GAO Guan-wei,LIU Ping-xiang,et al.Determination of 16 polycyclic aromatic hydrocarbons in tea by simultaneous dispersive solid-phase extraction and liquid-liquid extraction coupled with gas chromatography-tandem mass spectrometry[J].Food Analytical Methods,2016,9(8):2 374-2 384.
[6] 路杨,刘印平,王丽英,等.高风险食品中多环芳烃含量调查与分析[J].食品安全质量检测学报,2020,11(18):6 608-6 613.LU Yang,LIU Yin-ping,WANG Li-ying,et al.Investigation and analysis of polycyclic aromatic hydrocarbons content in high riskfood[J].Journal of Food Safety and Quality,2020,11(18):6 608-6 613.
[7] 胡玉霞,常福厚,白图雅,等.多环芳烃类化合物及芳香烃受体在肿瘤发生发展中的作用[J].中国生化药物杂志,2015,35(6):185-188.HU Yu-xia,CHANG Fu-hou,BAI Tu-ya,et al.Role of polycyclic aromatic hydrocarbons and aryl hydrocarbon receptor in the development of tum6or[J].Chinese Journal of Biochemical Pharmaceutics,2015,35(6):185-188.
[8] DEBAJYOTI G,SHREYA G,DUTTA T K,et al.Current state of knowledge in microbial degradation of polycyclic aromatic hydrocarbons(PAHs):A review[J].Frontiers in Microbiology,2016,7:1 369.
[9] ZHANG Yan-yan,DONG Si-jun,WANG Hong-ou,et al.Biological impact of environmental polycyclic aromatic hydrocarbons(ePAHs)as endocrine disruptors[J].Environmental Pollution,2016,213(6):809-824.
[10] SHI Long-kai,ZHANG Dong-dong,LIU Yu-lan.Survey of polycyclic aromatic hydrocarbons of vegetable oils and oilseeds by GC-MS in China[J].Food Addit & Contam,Part A,Chem,Anal,Control,Expo & Risk Assess,2016,33(4):603-611.
[11] 杜瑞,万丽斌,高火亮,等.固相萃取—高效液相色谱法检测植物油中15种多环芳烃[J].粮食与油脂,2022,35(1):158-162.DU Rui,WAN Li-bin,GAO Huo-liang,et al.Determination of 15 polycyclic aromatic hydrocarbons in vegetable oils by solid phase extraction coupled with high performance liquid chromatography[J].Cereals & Oils,2022,35(1):158-162.
[12] 何婷婷,周彤,谭婷,等.超声波辅助/疏水性低共熔溶剂萃取—高效液相色谱法测定牛奶中的多环芳烃[J].分析科学学报,2020,36(2):177-182.HE Ting-ting,ZHOU Tong,TAN Ting,et al.Determination of polycyclic aromatic hydrocarbons in milk by ultrasonic-assisted hydrophobic deep eutectic solvent extraction combined with high performance liquid chromatography[J].Journal of Analytical Science,2020,36(2):177-182.
[13] 傅孝美,李宗军,赵志友,等.食用槟榔中苯并芘检测方法研究进展[J].食品与机械,2019,35(8):216-225.FU Xiao-mei,LI Zong-jun,ZHAO Zhi-you,et al.Research progress of determination of benzo-a-pyrene in edible areca nut[J].Food & Machinery,2019,35(8):216-225.
[14] 蔡洁云,王惠平,刘巍,等.胶基型嚼烟中19种多环芳烃的气相色谱—串联质谱技术法测定[J].食品与机械,2020,36(5):89-94.CAI Jie-yun,WANG Hui-ping,LIU Wei,et al.Determination of polycylic aromatic hydrocarbons in gum-based chewing tobacco by GC-MS/MS[J].Food & Machinery,2020,36(5):89-94.
[15] 潘晓玉,王宗义,赵逸涵,等.冷冻脱脂—分散固相萃取/气相色谱-串联质谱法检测食用植物油中4种多环芳烃[J].分析试验室,2022,41(4):419-423.PAN Xiao-yu,WANG Zong-yi,ZHAO Yi-han,et al.Determination of 4 kinds of PAHs in edible plant oil by gas chromatography-tandem mass spectrometry with frozen defatting-dispersive solid-phase extraction[J].Chinese Journal of Analysis Laboratory,2022,41(4):419-423.
[17] 沈习习,战俊良,汤晓艳.凝胶渗透色谱净化—气相色谱—质谱法检测烤鸭鸭皮中16种多环芳烃[J].肉类研究,2020,34(1):77-82.SHEN Xi-xi,ZHAN Jun-liang,TANG Xiao-yan.Determination of 16 polycyclic aromatic hydrocarbons in roast duck skin by gel permeation chromatography clean-up combined with gas chromatography-mass spectrometry[J].Meat Research,2020,34(1):77-82.
[18] 孙志高,盛冉,郝静梅,等.QuEChERS-高效液相色谱法检测柑桔中毒死蜱、吡虫啉和残杀威农残[J].食品与机械,2017,33(6):68-72.SUN Zhi-gao,SHENG Ran,HAO Jing-mei,et al.Detected chlorpyrifos,imidacloprid,and propoxur residues in citrus by QuEChERS-high perfoemance liquid chromatography method[J].Food & Machinery,2017,33(6):68-72.
[19] WU Ling,HE Qiu-rong,ZHANG Jing,et al.QuEChERS with ultrasound-assisted extraction combined with high-performance liquid chromatography for the determination of 16 polycyclic aromatic hydrocarbons in sediment[J].Journal of AOAC International,2021,104(5):1 255-1 263.
[20] 李莎,曾习文,李亦蔚,等.QuEChERS-气相色谱—三重四极杆质谱法同时测定枸杞中46种农药残留[J].食品工业科技,2020,41(10):244-249.LI Sha,ZENG Xi-wen,LI Yi-wei,et al.Simultaneous determination of 46 pesticide residues in lycium barbarum by QuEChERS method combined with gas chromatography-triple quadrupole mass spectrometry[J].Science and Technology of Food Industry,2020,41(10):244-249.
[21] 易守福,梁锋,何青科,等.QuEChERS-气相色谱—三重四极杆质谱法同时测定黑茶中10种酰胺类除草剂残留[J].食品与机械,2021,37(8):57-62.YI Shou-fu,LIANG Feng,HE Qing-ke,et al.Simultaneous determination of 10 kinds of amide herbicide residues in dark tea by QuEChERS with gas chromatography-triple quadrupole mass spectrometry[J].Food & Machinery,2021,37(8):57-62.

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