Objective: This study aimed to obtain a novel fermented green tea with high ACE-inhibitory activity by Eurotium cristatum and to investigate its acute hypotensive effects on spontaneously hypertensive rats (SHR). Methods: The effects of pueraria addition, water content of substrate and spore suspension on ACE-inhibitory activity in vitro were investigated, and the fermentation process was optimized by orthogonal design experiment. The fermented tea solution was prepared by water extraction method, and SHR model was selected to study the hypotensive effect of fermented tea. Results: The optimal fermentation process for fermented tea was as follows: adding 2 g pueraria into 20 g green tea matrix, controlling the water content of the matrix to 20%, inoculatin 5% suspension of E. cristatum. Compared with the control group, different doses of fermented tea had lowering effects on blood pressure in SHR within 24 h. Conclusion: The acute hypotensive effect of the daily dose of 300~600 mg/kg was the most significant, which could lower the blood pressure of SHR in a short period of time.

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[1] MAHDAVI-ROSHAN Marjan,SALARI Arsalan,GHORBANI Zeinab,et al.The effects of regular consumption of green or black tea beverage on blood pressure in those with elevated blood pressure or hypertension:A systematic review and meta-analysis[J].Complementary Therapies in Medicine,2020,51:11.
[2] CHEN Yu-lian,WANG Yuan-liang,CHEN Jia-xu,et al.Bioprocessing of soybeans(Glycine maxL.)by solid-state fermentation with Eurotium cristatumYL-1 improves total phenolic content,isoflavone aglycones,and antioxidant activity[J].RSC Advances,2020,10(29):16 928-16 941.
[3] XIAO Yue,LI Mao-yun,LIU Ya,et al.The effect of Eurotium cristatum(MF800948)fermentation on the quality of autumn green tea[J].Food Chemistry,2021,358:129848.
[4] XIAO Yue,ZHONG Kai,BAI Jin-rong,et al.The biochemical characteristics of a novel fermented loose tea by Eurotium cristatum(MF800948)and its hypolipidemic activity in a zebrafish model[J].LWT,2020,117:108629.
[5] MUSIAL Claudia,KUBAN-JANKOWSKA Alicja,GORSKA-PONIKOWSKA Magdalena.Beneficial properties of green tea catechins[J].International Journal of Molecular Sciences,2020,21(5):1 744.
[6] DONG Jun-jie,XU Xin-qing,LIANG Yue-rong,et al.Inhibition of angiotensin converting enzyme(ACE)activity by polyphenols from tea(Camellia sinensis)and links to processing method[J].Food & Function,2011,2(6):310-319.
[7] 李心芯,闵二虎,王蓓,等.葛根提取物对高盐饮食小鼠血压和肠道菌群的影响[J].营养学报,2020,42(5):491-496,504.LI Xin-xin,MIN Er-hu,WANG Bei,et al.Effect of pueraria extract on salt-sensitive hypertension and gut microbiota in mice[J].Acta Nutrimenta Sinica,2020,42(5):491-496,504.
[8] 权赫秀,李巧巧,张普照,等.葛根和粉葛对L-NAME诱导高血压大鼠的作用比较及机制研究[J].中药材,2020,43(11):2 773-2 778.QUAN He-xiu,LI Qiao-qiao,ZHANG Pu-zhao,et al.Comparison and mechanism of puerariae lobatae radix and puerariae thomsonii radix on L-NAME induced hypertension in rats[J].Journal of Chinese Medicinal Materials,2020,43(11):2 773-2 778.
[9] 龚受基,滕翠琴,曹惠怡,等.冠突散囊菌对夏秋绿茶发酵的影响[J].食品科技,2020,45(1):134-140.GONG Shou-ji,TENG Cui-qin,CAO Hui-yi,et al.Influences on summer-autumn tea fermented with Eurotium Cristatum[J].Food Science and Technology,2020,45(1):134-140.
[10] ZHANG Bo,LU Zhou,XU Xiao,et al.Solid-state fermentation with Eurotium cristatumHC-18 o improve antioxidant activity of kudzu(Pueraria lobata)root[J].Journal of Food and Nutrition Research,2018,57(4):384-395.
[11] XIAO Yue,ZHONG Kai,BAI Jin-Rong,et al.Insight into effects of isolated Eurotium cristatumfrom Pingwu Fuzhuan brick tea on the fermentation process and quality characteristics of Fuzhuan brick tea[J].Journal of the Science of Food and Agriculture,2020,100(9):3 598-3 607.
[12] 曹盼,张樱山,魏学明,等.葛根素药理作用研究新进展[J].中成药,2021,43(8):2 130-2 134.CAO Pan,ZHANG Ying-shan,WEI Xue-ming,et al.Progress on the pharmacological research of Puerarin[J].Chinese Traditional Patent Medicine,2021,43(8):2 130-2 134.
[13] 杜静,王琪琪,王云胜,等.冠突散囊菌发酵对葛根的活性物质和抗氧化活性的影响[J].食品工业科技,2021,42(1):121-125,131.DU Jing,WANG Qi-qi,WANG Yun-sheng,et al.Effect onbioactive components and antioxidant activity of pueraria lobata fermented by Eurotium cristatum[J].Science and Technology of Food Industry,2021,42(1):121-125,131.
[14] LIU Lu,SHI Jia-jun,YUE Tian-li,et al.Changes in the metabolite composition and enzyme activity of fermented tea during processing[J].Food Research International,2022,158:111428.
[15] ZOU Min-min,GUO Xiao-han,HUANG Yan,et al.Improvement of the quality of Ginkgo biloba leaves fermented byEurotium cristatumas high value-added feed[J].Processes,2019,7(9):627.
[16] 李红胜,邢宏博,许赣荣,等.红曲菌固态发酵产消化酶生产工艺优化[J].广东农业科学,2021,48(11):133-142.LI Hong-sheng,XING Hong-bo,XU Gan-rong,et al.Optimization of production process of digestive enzyme from solid-state fermentation by Monascus[J].Guangdong Agricultural Sciences,2021,48(11):133-142.
[17] 姜良珍,王罗,杨涛,等.冠突散囊菌及其发酵应用研究进展[J].食品工业科技,2022,43(4):454-462.JIANG Liang-zhen,WANG Luo,YANG Tao,et al.Researchprogress on Eurotium cristatumand its fermentation application[J].Science and Technology of Food Industry,2022,43(4):454-462.
[18] 邬秀宏,王杰,杨海滨,等.绿茶对SHR大鼠生长性能和血液生化指标的影响[J].南方农业,2018,12(28):25-28,63.WU Xiu-hong,WANG Jie,YANG Hai-bin,et al.Effects of green tea on SHR growth performance and blood biochemical indices[J].South China Agriculture,2018,12(28):25-28,63.
[19] 罗显扬,周国兰,梁月荣,等.“高原明珠”绿茶对自发性高血压大鼠血压、心肌超微结构及血管紧张素Ⅱ含量的影响[J].西南农业学报,2011,24(5):1 977-1 980.LUO Xian-yang,ZHOU Guo-lan,LIANG Yue-rong,et al.Effects of 'Plateau Bright Pearl' green tea on blood pressure,myocardial microstructure and level of plasma Angiotensin Ⅱ(Ang Ⅱ)in spontaneous hypertensive rats[J].Southwest China Journal of Agricultural Sciences,2011,24(5):1 977-1 980.
[20] 李汝月,刘佩,胡俊杰,等.霉茶总黄酮对自发性高血压大鼠肾脏的保护作用及机制研究[J].时珍国医国药,2020,31(7):1 537-1 539.LI Ru-yue,LIU Pei,HU Jun-jie,et al.Protective effect and mechanism of Meicha total flavonoids on kidney in spontaneously hypertensive rats[J].Lishizhen Medicine and Materia Medica Research,2020,31(7):1 537-1 539.
[21] 蒋荣华,候小利,王硕,等.甜茶多酚对自发性高血压大鼠的影响[J].世界科学技术:中医药现代化,2015,17(7):1 479-1 485.JIANG Rong-hua,HOU Xiao-li,WANG Shuo,et al.Effect of polyphenols from Rubus suavissirnu S.lee on spontaneous hypertensive rats[J].World Science and Technology:Modernization of Traditional Chinese Medicine,2015,17(7):1 479-1 485.
[22] 周尧,童华荣.模拟体外消化评定超微绿茶粉的冲泡饮用效果[J].食品与机械,2020,36(11):172-176.ZHOU Yao,TONG Hua-rong.Evaluation of the brewing effect of ultrafine green tea powder by simulated in vitro digestion[J].Food & Machinery,2020,36(11):172-176.



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