Objective: The study aimed to develop the vegetable value of common vetch (CV) and explore the effects of salt solution on phenolic accumulation and antioxidant activity in CV sprouts.Methods: The effects of NaCl stress and supplemental CaCl2 on free and combined phenolic accumulation in CV sprouts were analysed by UPLC in comparison with pea sprouts, soybean sprouts and mung bean sprouts. The changes of key enzyme activities and gene relative expression levels of PAL, C4H and 4CL, antioxidant enzyme activities and antioxidant activities were investigated under NaCl and CaCl2 treatments.Results: The total phenolics increased from (10.63±0.40) mg GAE/g DW to (15.76±0.36) mg GAE/g DW with the co-treatment of NaCl and CaCl2. Sixteen phenolic acids were detected in CV sprouts. The content of Rutin increased from (22.50±0.49) mg/kg DW to (57.38± 0.87) mg/kg DW with the co-treatment, which was 79.69, 260.82, 5.89 times of pea sprout, soybea sprout and mung bean sprout respectively. Na+ increased enzyme activities of PAL,4CL and C4H by increasing their relative gene expression levels. However, the co-treatment increased enzyme activities but their relative gene expression levels decreased or didnʼt change significantly.Conclusion: The co-treatment of NaCl and CaCl2 can enrich the phenolic content and enhance its antioxidant activity of CV sprouts.

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[1] DRAGICEVIC V,JANOSEVIC B,BRANKOV M,et al.Enhanced nutritional quality of sweet maize kernel in response to cover crops and bio-Fertilizer[J].Agronomy,2021,11:981.
[2] LUO Cai-yun,WANG Shi-ping,ZHAO Liang,et al.Seeding ratios and phosphate fertilizer on ecosystem carbon exchange of common vetch and oat[J].Nutrient Cycling in Agroecosystems,2017,109:149-160.
[3] PASTOR-CAVADA E,JUAN R,PASTOR J E,et al.Antioxidantive activity in the seeds of 28 vicia speicies from southern spain[J].Journal of Food Biochemistry,2011,35(5):1 373-1 380.
[4] GHARACHORLOO M,TARZI B G,BAHARINIA M.The Effect of germination on phenolic compounds and antioxidant activity of pulses[J].Journal of the American Oil Chemists Society,2013,90(3):407-411.
[5] MEGÍAS C,PASTOR-CAVADA E,TORRES-FUENTES C,et al.Chelating,antioxidant and antiproliferative activity of Vicia sativa polyphenol extracts[J].European Food Research & Technology,2009,230(2):353-359.
[6] MAGALHAES S C Q,TAVEIRA M,CABRITA A R J,et al.European marketable grain legume seeds:Further insight into phenolic compounds profiles[J].Food Chemistry,2017,215(15):177-184.
[7] GUETA-DAHAN Y,YANIV Z,BEN-HAYYIM Z G.Salt and oxidative stress:Similar and specific responses and their relation to salt tolerance in Citrus[J].Planta,1997,203(4):460-469.
[8] GOHARRIZI K J,RIAHI-MADVAR A,REZAEE F,et al.Effect of salinity stress on enzymes' activity,ions concentration,oxidative stress parameters,biochemical traits,content of sulforaphane,and CYP79F1 gene expression level in lepidium draba plant[J].Journal of Plant Growth Regulation,2020,39(3):1 075-1 094.
[9] KIM N S,KIM J K,SATHASIVAM R,et al.Impact of betaine under salinity on accumulation of phenolic compounds in safflower(Carthamus tinctorius L.)sprouts[J].Natural Product Communications,2021,16(5):1934578X2110150.
[10] WANG Mian,DING Yu-xuan,WANG Qiao-e,et al.NaCl treatment on physio-biochemical metabolism and phenolics accumulation in barley seedlings[J].Food Chemistry,2020,331:127282.
[11] WANG Xin-kun,YANG Run-qiang,ZHOU Yu-lin,et al.A comparative transcriptome and proteomics analysis reveals the positive effect of supplementary Ca 2+on soybean sprout yield and nutritional qualities[J].Journal of Proteomics,2016,143:161-172.
[12] WANG Xin-kun,YANG Run-qiang,JIN Xiao-lin,et al.Effect of supplemental Ca 2+ on yield and quality characteristics of soybean sprouts[J].Scientia Horticulturae,2016,198:352-362.
[13] MA Yan,WANG Pei,ZHOU Ting,et al.Role of Ca 2+ in phenolic compound metabolism of barley(Hordeum vulgare L.)sprouts under NaCl stress[J].Journal of the Science of Food and Agriculture,2019,99(11):5 176-5 186.
[14] COFFIGNIEZ F,RYCHLIK M,MESTRES C,et al.Modelling folates reaction kinetics during cowpea seed germination in comparison with soaking[J].Food Chemistry,2020,340:127960.
[15] RAO Huan,XUE Feng,MA Su-hong,et al.Contribution of slightly acidic electrolytic water(SAEW)to food safety,nutrients enrichment and allergenicity reduction of peanut sprouts[J].Journal of Food Processing and Preservation,2022,46(3):e16396.
[16] 陈益胜,舒蓝萍,徐学明,等.3 种藜麦发芽过程中生物活性物质及其抗氧化活性的变化规律[J].食品与机械,2020,36(3):34-38.CHEN Yi-sheng,SHU Lan-ping,XU Xue-ming,et al.Changes of bioactive substances and antioxidant properties of three kinds of quioa during germination[J].Food & Machinery,2020,36(3):34-38.
[17] SWIECA M,GAWLIK-DZIKI U,KOWALCZYK D,et al.Impact of germination time and type of illumination on the antioxidant compounds and antioxidant capacity of Lens culinaris sprouts[J].Scientia Horticulturae,2012,140:87-95.
[18] 马燕.NaCl胁迫下GABA介导的大麦芽苗酚酸富集机理[D].南京:南京农业大学,2019:28-30.MA Yan.The mechanism of phenolic acid accumulation mediated by GABA in barley seedlings under NaCl stress[D].Nanjing:Nanjing Agricultural University,2019:28-30.
[19] DEETAE P,PARICHANON P,TRAKUNLEEWATTHANA P,et al.Antioxidant and anti-glycation properties of Thai herbal teas in comparison with conventional teas[J].Food Chemistry,2012,133(3):953-959.
[20] 黄彪,何伟,吴建鸿,等.UPLC-MS/MS同时测定铁皮石斛茎,叶,花中酚类组分的含量[J].食品科学,2021,42(10):262-268.HUANG Biao,HE Wei,WU Jian-hong,et al.Simultaneous determination of phenolic components in dendrobium officinale stems,leaves and flowers by ultra-high performance liquid chromatography-tandem mass spectrometry[J].Food Chemistry,2021,42(10):262-268.
[21] ZHANG Zhan-quan,TIAN Shi-ping,ZHU Zhu,et al.Effects of 1-methylcyclopropene(1-MCP)on ripening and resistance of jujube(Zizyphus jujuba cv.Huping)fruit against postharvest disease[J].LWT-Food Science and Technology,2012,45(1):13-19.
[22] CHANCE B,MAEHLY A C.Assay of catalases and peroxidases[J].Methods in Enzymology,1955(2):764-775.
[23] 陈雷,常丽,曹福亮,等.银杏叶黄酮类化合物含量及相关酶活性对温度和干旱胁迫的响应[J].西北植物学报,2013,33(4):755-762.CHEN Lei,CHANG Li,CAO Fu-liang,et al.Effects of temperature and soil water deficit on the flavonoid content and activities of enzymes involved in ginkgo leaves[J].Acta Botanica Sinica of Northwest China,2013,33(4):755-762.
[24] KOODKAEW I.NaCl and glucose improve health-promoting properties in mung bean sprouts[J].Scientia Horticulturae,2019,247:235-241.
[25] ISLAM M Z,PARK B J,KANG H M,et al.Influence of selenium biofortification on the bioactive compounds and antioxidant activity of wheat microgreen extract[J].Food Chemistry,2020,309:125763.
[26] 陆胜波.铁核桃果实青皮和内种皮芦丁合成的转录组与代谢组分析[D].贵阳:贵州大学,2020:8-11.LU Sheng-bo.Transcriptomes and metabonomics profiling reveals rutin biosynthesis of green husk and kernel pellicle in walnut fruit(Juglans sigillata Dode.)[D].Guiyang:Guizhou University,2020:8-11.
[27] 唐文文,夏俊丽,陈垣.铁皮石斛茎、叶、花功能性成分、抗氧化活性及其相关性[J].食品与机械,2021,37(7):45-50.TANG Wen-wen,XIA Jun-li,CHEN Huan.Analysis of functional compositon,antioxidant activity and their correlation in stem,leaf and flower from Dendrobium officinale[J].Food & Machinery,2021,37(7):45-50.
[28] CHEN Lin,TAN Jun-tong,ZHAO Xue,et al.Energy regulated enzyme and non-enzyme-based antioxidant properties of harvested organic mung bean sprouts(Vigna radiata)[J].LWT,2019,107:228-235.

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