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Corresponding Author(s)

王立(1978—),男,江南大学食品学院教授,博士。E-mail:wangli0519@163.com

Abstract

Objective: This research aimed to study the preparation of standardized and stable sourdough starter, to explore the effects of different drying processes and protective agents on the survival rate of sourdough, and examine its storage activity. Methods: A variety of fruits were selected to prepare sourdough, the fermentation process was studied, and the strains were isolated and identified by ITs and 16s gene sequencing in the database. The optimum scheme was obtained by selecting the drying process, sample state and protectant formulation with the activity after drying and storage activity as indexes. Results: It was found that the bread prepared with grape sourdough had the best specific volume, porosity and hardness. On this basis, the drying survival rate and storage activity were used as indexes to study the combination of drying protectants. Finally, two better protective agent formulations were obtained by orthogonal experiment: ① Freeze-dried the dominant pure bacterial solution, skim milk 35%, maltodextrin 7.5% and trehalose 25%, and the drying survival rate was about 93%. ② Spray dried sourdough germ whole, skim milk 35%, maltodextrin 10% and trehalose 15%, and the drying survival rate was about 90%. Conclusion: It was found that the storage activity of the spray-dried starter decreased rapidly, while that of the freeze-dried starter was slower.

Publication Date

3-27-2024

First Page

197

Last Page

203,212

DOI

10.13652/j.spjx.1003.5788.2023.60132

References

[1] VANDEVIJVERE S, JAACKS L M, MONTEIRO C A, et al. Global trends inultraprocessed food and drink product sales and their association with adult body mass index trajectories[J]. Obesity Reviews, 2019, 2(20): 10-19.
[2] D'ALESSANDRO A, LAMPIGNANO L, DE P G. Mediterranean diet pyramid: A proposal for Italian people. A systematic review of prospective studies to derive serving sizes[J]. Nutrients, 2019, 11(6): 1 296.
[3] GOBBETTI M, CORSETTI A, ROSSI J. The sourdough microflora: Interactions of lactic acid bacteria and yeasts[J]. World Journal of Microbiology and Biotechnology, 1994, 10(3): 275-279.
[4] BRANDT M J. Industrial production of sourdoughs for the baking branch-an overview[J]. International Journal of Food Microbiology, 2019, 302: 3-7.
[5] SUBASI A S, ERCAN R. The effects of wheat variety, sourdough treatment and sourdough level on nutritional characteristics of whole wheat bread[J]. Journal of Cereal Science, 2023, 110: 103637.
[6] PEJCZ E, LACHOWICZ-WISNIEWSKA S, NOWICKA P, et al. Effect of inoculated lactic acid fermentation on the fermentable saccharides and polyols, polyphenols and antioxidant activity changes in wheat sourdough[J]. Molecules, 2021, 26(14): 4 193.
[7] FILANNINO P, DI CAGNO R, GOBBETTI M. Metabolic and functional paths of lactic acid bacteria in plant foods: Get out of the labyrinth[J]. Current Opinion in Biotechnology, 2018, 49: 64-72.
[8] 邹奇波, 黄卫宁, 程新, 等. 混菌发酵酸面团对全麦面包风味与烘焙特性的影响[J]. 食品与机械, 2020, 36(4): 32-39. ZOU Q B, HUANG W N, CHENG X, et al. Effects on flavor and baking characteristics of whole wheat bread by fermented sourdough with the mixed culture[J]. Food & Machinery, 2020, 36(4): 32-39.
[9] 曹伟超, 周黎源, 罗昆, 等. 高产植酸酶乳酸菌及其黑豆酸面团发酵低植酸营养面包研究[J]. 食品与机械, 2021, 37(2): 186-193. CAO W C, ZHOU L Y, LUO K, et al. Studies on screening of high-yield phytase-producing lactic acid bacteria and its low-phytate nutritional breads through black bean sourdough fermentation[J].Food & Machinery, 2021, 37(2): 186-193.
[10] PEREZ-ALVARADO O, ZEPEDA-HERNANDEZ A, GARCIA-AMEZQUITA L E, et al. Role of lactic acid bacteria and yeasts in sourdough fermentation during breadmaking: Evaluation of postbiotic-like components and health benefits[J].Frontiers in Microbiology, 2022, 13: 969460.
[11] GOBBETTI M. The sourdough microflora: Interactions of lactic acid bacteria and yeasts[J]. Trends in Food Science & Technology, 1998, 9(7): 267-274.
[12] DE VUYST L, VAN KERREBROECK S, LEROY F. Microbial ecology and process technology of sourdough fermentation[J]. In Advances in Applied Microbiology, 2017, 100: 49-160.
[13] GALANAKIS C M. Innovations in traditional foods[M]. Duxford, United Kingdom: Woodhead Publishing, 2019: 14-40.
[14] 虞桠芳. 水果天然酵母发酵特性及其对面包烘焙品质的影响[D]. 无锡: 江南大学, 2018: 5-40. YU Y F. Studies on leavening properties of fruit sourdoughs and their effects on baking quality of bread[D]. Wuxi: Jiangnan University, 2018: 5-40.
[15] YU Y F, WANG L, QIAN H F, et al. Contribution of spontaneously-fermented sourdoughs with pear and navel orange for the bread-making[J]. LWT-Food Science and Technology, 2018, 89: 336-343.
[16] FANG L P, WANG W J, DOU Z X, et al. Effects of mixed fermentation of different lactic acid bacteria and yeast on phytic acid degradation and flavor compounds in sourdough[J]. LWT-Food Science and Technology, 2023, 174: 114438.
[17] 刘若诗. 乳酸菌酸面团发酵剂的制备及其发酵烘焙特性研究[D]. 无锡: 江南大学, 2010: 5-35. LIU R S. Studies on preparation of lactic acid sourdough starters and their fermentation and baking properties[D]. Wuxi: Jiangnan University, 2010: 5-35.
[18] BUKSA K, KOWALCZYK M, BORECZEK J. Extraction, purification and characterisation of exopolysaccharides produced by newly isolated lactic acid bacteria strains and the examination of their influence on resistant starch formation[J]. Food Chemistry, 2021, 362: 130221.
[19] GALLI V, MAZZOLI L, LUTI S, et al. Effect of selected strains of lactobacilli on the antioxidant and anti-inflammatory properties of sourdough[J]. Int J Food Microbiol, 2018, 286: 55-65.
[20] BESTED A C, LOGAN A C, SELHUB E M. Intestinal microbiota, probiotics and mental health: From Metchnikoff to modern advances: Part II-contemporary contextual research[J].Gut Pathogens, 2013, 5: 3.
[21] MIRANDA R F, DE PAULA M M, DA COSTA G M, et al. Orange juice added with L-casei: Is there an impact of the probiotic addition methodology on the quality parameters?[J]. LWT-Food Science and Technology, 2019, 106: 186-193.
[22] HUANG S, VIGNOLLES M L, CHEN X D, et al. Spray drying of probiotics and other food-grade bacteria: A review[J]. Trends in Food Science & Technology, 2017, 63: 1-17.
[23] ASSADPOUR E, JAFARI S M. Advances in spray-drying encapsulation of food bioactive ingredients: From microcapsules tonanocapsules[J]. In Annual Review of Food Science and Technology, 2019, 10: 103-131.
[24] 姜甜, 陆文伟, 崔树茂, 等. 不同微囊化方法包埋双歧杆菌菌粉特性分析[J]. 食品工业科技, 2021, 42(11): 128-134. JIANG T, LU W W, CUI M S, et al. Characteristic analysis of different micro encapsulated Bifidobacterium[J]. Institute of Food Biotechnology, 2021, 42(11): 128-134.
[25] ZHANG Z, PENG S, SUN X Q, et al. A novel insight to screen the optimal spray-drying protectants and parameters for manufacturing lactic acid bacteria preparations[J].Drying Technology, 2020, 38(14): 1 843-1 856.
[26] STEFANELLO R F, MACHADO A A R, CAVALHEIRO C P, et al. Trehalose as a cryoprotectant in freeze-dried wheat sourdough production[J]. LWT-Food Science and Technology, 2018, 89: 510-517.
[27] CAGLAR N, ERMIS E, DURAK M Z. Spray-dried and freeze-dried sourdough powders: Properties and evaluation of their use in breadmaking[J]. Journal of Food Engineering, 2021, 292: 110355.

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