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Corresponding Author(s)

洪键(1981—),男,盐城师范学院高级实验师,博士。E-mail:jian_hong602@sina.com

Abstract

Objective: This study aimed to optimize the process parameters of fermentative Cynanchum auriculatum (CA) tablets to develop a new type of fermented product of CA. Methods: Taking complete slices of CA as raw materials, the process parameters of fermentative CA tablets were optimized by response surface methodology based on single factor experiments with sensory score and total acid content as indicators, and the antioxidant activity and amino acid content of fermentative CA tablets were tested. Results: The optimal process parameters for fermentative CA tablets were material liquid at ratio 1∶1, fermentation at 31 ℃, inoculation amount 9.6% and fermenting for 25 h. The sensory score was 89.33, and the total acid content was 0.726 g/100 g under the control of theoptimal processing conditions. At the concentration of 10 mg/mL, the total antioxidant capacity of the fermentative CA tablets alcohol extract was 2.05 U/mL, and the scavenging rate of DPPH and ABTS+ free radicals was 32.18% and 45.89% respectively. Test results of the amino acid content showed that the content of aromatic amino acids in CA tablets increased by 47.22% after fermentation. Conclusion: Fermentative CA tablets obtained under the control of the optimal processing conditions have complete morphology and structure, uniform color, harmonious sourness and sweetness, pleasant wine aroma and characteristic flavor of CA and high antioxidant activity, indicating the product has achieved the expected effect.

Publication Date

3-27-2024

First Page

204

Last Page

212

DOI

10.13652/j.spjx.1003.5788.2023.80647

References

[1] CHEN W H, ZHANG Z Z, BAN Y F, et al. Cynanchum bungei Decne and its two related species for "Baishouwu": A review on traditional uses, phytochemistry, and pharmacological activities[J]. Journal of Ethnopharmacology, 2019, 243: 1-23.
[2] 张明, 沈明晨, 陈镭, 等. 白首乌的化学成分、药理作用及栽培技术综述[J]. 江苏农业科学, 2022, 50(2): 22-29. ZHANG M, SHEN M C, CHEN L, et al. On chemical constituent, pharmacological effect and cultivation techniques of Cynanchum bungei: A review[J]. Jiangsu Agricultural Sciences, 2022, 50(2): 22-29.
[3] 陈其燕, 石云, 洪键. 滨海白首乌多糖的提取与药理活性研究进展[J]. 现代农业科技, 2020(19): 215-216, 220. CHEN Q Y, SHI Y, HONG J. Research progress on extraction and pharmacological activities of polysaccharides in Cynanchum auriculatum[J]. Modern Agricultural Science and Technology, 2020(19): 215-216, 220.
[4] 李青, 董兆稀, 赵冰清. 白首乌多糖抗疲劳及耐缺氧、耐高温作用研究[J]. 湖南师范大学自然科学学报, 2012, 35(5): 76-79. LI Q, DONG Z X, ZHAO B Q. Effects of radix Cynanchi bungei polysaccharides on anti-fatigue, hypoxia tolerance and heat-resisting in mice[J]. Journal of Natural Science of Hunan Normal University, 2012, 35(5): 76-79.
[5] 陈亚, 徐晓燕. 白首乌的生物学特性及标准化栽培技术研究[J]. 时珍国医国药, 2014, 25(7): 1 730-1 731. CHEN Y, XU X Y. Study on biological characteristics and standardized cultivation techniques of Cynanchum auriculatum[J]. Lishizhen Medicine and Materia Medica Research, 2014, 25(7): 1 730-1 731.
[6] 宋婉钰, 李勇, 李春阳, 等. 基于网络药理学及代谢产物分析白首乌对乙醇性肝损伤的作用机制[J]. 江苏农业科学, 2023, 51(9): 166-174. SONG W Y, LI Y, LI C Y, et al. Study on mechanism of Cynanchum auriculatum on alcoholic liver disease based on network pharmacology and metabolite analysis[J]. Jiangsu Agricultural Sciences, 2023, 51(9): 166-174.
[7] 王永青, 李光荣, 刘华. 绿色防控技术在江苏滨海白首乌上的示范应用与推广[J]. 农业装备技术, 2022, 48(6): 33-35. WANG Y Q, LI G R, LI H. Demonstration application and promotion of green prevention and control technology on Jiangsu Cynanchum auriculatum[J]. Agricultural Equipment & Technology, 2022, 48(6): 33-35.
[8] 徐华益. 白首乌发酵乳的制备及其抗氧化功效特性的研究[D]. 扬州: 扬州大学, 2020: 53. XU H Y. Study on preparation of CaREW fermented milk and its antioxidant properties[D]. Yangzhou: Yangzhou University, 2020: 53.
[9] CHAI Z, HUANG W Y, ZHAO X, et al. Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of polysaccharide from Cynanchum auriculatum Royle ex Wight[J]. International Journal of Biological Macromolecules, 2018(119): 1 068-1 076.
[10] JI C X, LI X Y, JIA S B, et al. The antidepressant effect of Cynanchum auriculatum in mice[J]. Pharmaceutical Biology, 2012, 50(9): 1 067-1 072.
[11] WANG X J, LI Z L, LU X H, et al. Antitumor evaluation and multiple analysis on different extracted fractions of the root of Cynanchum auriculatum Royle ex Wight[J]. Journal of Separation Science, 2017, 40(15): 3 054-3 063.
[12] WU Z H, WANG Y Y, MENG X, et al. Total C-21 steroidal glycosides, isolated from the root tuber of Cynanchum auriculatum Royle ex Wight, attenuate hydrogen peroxide-induced oxidative injury and inflammationin L02 cells[J]. International Journal of Molecular Medicine, 2018, 42(6): 3 157-3 170.
[13] 罗珍岑. 乳酸发酵制备火棘果咀嚼片品质特性及功能作用研究[D]. 重庆: 西南大学, 2021: 10-12. LUO Z C. Research on the quality characteristics and functional effects of chewable tablets of lactic acid fermented Pyracantha fortuneana fruit[D]. Chongqing: Southwest University, 2021: 10-12.
[14] 白莉圆. 麸皮红枣乳酸发酵片加工工艺研究[D]. 西安: 陕西师范大学, 2019: 25-55. BAI L Y. Study on processing technology of bran and jujube lactic acid fermented tablet[D]. Xi'an: Shaanxi Normal University, 2019: 25-55.
[15] 叶汪阳, 叶红, 吴晓娟, 等. 一种木瓜果脯片、果浆的制备方法: CN103875873B[P]. 2015-05-20. YE W Y, YE H, WU X J, et al. A preparation method for papaya preserved slices and pulp: CN103875873B[P]. 2015-05-20.
[16] 李后如. 一种舒冠颗粒的制备方法: CN110624050B[P]. 2021-07-09. LI H R. A preparation method for Shuguan granules: CN110624050B[P]. 2021-07-09.
[17] 陈树俊, 郑婕. 响应面优化开菲尔粒发酵复合果蔬汁工艺及体外消化特性分析[J]. 食品研究与开发, 2020, 41(3): 150-158. CHEN S J, ZHENG J. Optimization of compound fruit and vegetable juice fermentation with kefir grain by response surface and analysis of digestion characteristics in vitro[J]. Food Research and Development, 2020, 41(3): 150-158.
[18] 薛乃峰. 利用多菌种发酵结合细胞固定化技术生产黑蒜复合发酵饮料工艺研究[D]. 济南: 齐鲁工业大学, 2017: 17-18. XUE N F. Production of black garlic compound fermented beverage by multi-strain fermentation combined with cell immobilization technology[D]. Jinan: Oilu University of Technology, 2017: 17-18.
[19] 吴虹艳, 孔令姝, 赵家乐, 等. 响应面法优化胡萝卜—发酵蓝莓奶片的工艺研究[J]. 食品工业科技, 2019, 40(15): 100-105. WU H Y, KONG L S, ZHAO J L, et al. Optimization of carrot-fermented blueberry milk tablet by response surface methodology[J]. Science and Technology of Food Industry, 2019, 40(15): 100-105.
[20] 陈文璐, 刘妍妍, 巩燕妮. 响应面法优化干酪风味发酵乳的发酵工艺[J]. 食品工业科技, 2021, 42(23): 235-242. CHEN W L, LIU Y Y, GON Y N. Optimization of fermentation processing of Chinese flavor fermented milk by response surface methodology[J]. Science and Technology of Food Industry, 2021, 42(23): 235-242.
[21] 冯康, 李爱民, 吴晓磊, 等. 玛咖多糖的提取条件及体外活性研究[J]. 食品科学技术学报, 2021, 39(2): 48-55. FENG K, LI A M, WU X L, et al. Study on optimization of maca polysaccharides extraction and in vitro antioxidant and hypolipidemic activities[J]. Journal of Food Science and Technology, 2021, 39(2): 48-55.
[22] 孙正霄, 肖顺丽, 刘陆, 等. 枸杞咀嚼片免疫调节及抗氧化活性分析[J]. 中国实验方剂学杂志, 2022, 28(8): 46-53. SUN Z X, XIAO L L, LIU L, et al. Immunomodulatory and antioxidant activity of Gouqi chewable tablets[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2022, 28(8): 46-53.
[23] 田欢, 裴龙英, 布海丽且姆·阿卜杜热合曼, 等. 乳酸菌发酵桑椹汁工艺优化及发酵过程中功能性成分及抗氧化活性的变化[J]. 食品工业科技, 2023, 44(23): 90-100. TIAN H, PEI L Y, BUHAILIQIEMU A, et al. Optimization of the fermentation process of mulberry juice by lactic acid bacteria and changes in functional components and antioxidant activity during fermentation[J]. Science and Technology of Food Industry, 2023, 44(23): 90-100.
[24] 刘畅, 左常洲, 彭菁, 等. 响应面优化植物乳杆菌发酵番茄汁工艺及其品质评估[J]. 食品工业科技, 2022, 43(10): 246-253. LIU C, ZUO C Z, PENG J, et al. Response surface optimization of the fermentation process of tomato juice by Lactobacillus plantarum and its quality evaluation[J]. Science and Technology of Food Industry, 2022, 43(10): 246-253.
[25] 颜飞翔, 朱丹, 牛广财, 等. 秋香梨果醋醋酸发酵工艺优化及抗氧化活性研究[J]. 食品与机械, 2021, 37(4): 184-188, 225. YAN F X, ZHU D, NIU G C, et al. Optimization of acetic acid fermentation process and antioxidant activity of Qiuxiang pear vinegar[J]. Food & Machinery, 2021, 37(4): 184-188, 225.
[26] 王玉玮, 袁亚宏, 岳田利. 雪莲菌发酵豇豆工艺优化及其挥发性风味成分分析[J]. 食品与机械, 2021, 37(5): 176-182, 232. WANG Y W, YUAN Y H, YUAN T L. Process optimization of fermented cowpea by kefir grains and analysis of volatile flavor components[J]. Food & Machinery, 2021, 37(5): 176-182, 232.
[27] 白桂英, 叶淑红, 王琛郴, 等. 黑枸杞乳酸菌饮料发酵工艺优化及功能性成分研究[J]. 中国酿造, 2022, 41(4): 157-162. BAI G Y, YE S H, WANG C C, et al. Fermentation process optimization and functional ingredients of black wolfberry lactic acid bacteria beverage[J]. China Brewing, 2022, 41(4): 157-162.
[28] 朱立斌, 朱丹, 牛广财, 等. 毛酸浆乳酸发酵工艺优化及其抗氧化活性[J]. 食品科技, 2020, 45(7): 50-56. ZHU L B, ZHU D, NIU G C, et al. Lactic acid fermentation condition optimization and antioxidant activity of Physalis Pubescens L.[J]. Food Science and Technology, 2020, 45(7): 50-56.
[29] 何婉莹, 黄展锐, 赵良忠, 等. 生浆法制作豆腐的工艺优化[J]. 现代食品科技, 2021, 37(10): 188-196. HE W Y, HUANG Z R, ZHAO L Z, et al. Optimization of Tofu production from filtered raw soybean milk[J]. Modern Food Science and Technology, 2021, 37(10): 188-196.
[30] 李志华. 产β-葡萄糖苷酶乳酸菌菌株的特性研究[D]. 延边: 延边大学, 2014: 5-7. LI Z H. Study on characteristics of lactic acid bacteria strains producing β-glucosidase[D]. Yanbian: Yanbian University, 2014: 5-7.
[31] SHAHIDI F, ALASALVAR, LIYANA PATHIRANA C M. Antioxidant phytochemicals in hazelnut kernel (Corylus avellana L.) and hazelnut byproducts[J]. Journal of Agriculture and Food Chemistry, 2007, 55(4): 1 212-1 220.
[32] SMID E, KLEEREBEZEM M. Production of aroma compounds in lactic fermentations[J]. Annual Review of Food Science and Technology, 2014, 5(1): 313-326.
[33] ARDO Y. Flavour formation by amino acid catabolism[J]. Biotechnology Advances, 2006, 24(2): 238-242.

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